FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488183

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488183
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences496527
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN24740.4982609203527703No Hit
CTTTTATATAAGATACCCGCCTTCCACAGAGTGTGTAAATAATGTCTCGT17810.35869147095726917No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC11650.23462973816126817No Hit
ATGTAATCAATTTGTGGGGCCCCTTACAGTAAATGAAAAAAGAAGACTAA11300.22758077607058627No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGAAACACCCGTG10800.21751083022675502No Hit
CTGTTGACGTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAAAAGCCTGTT9290.18709959377838464No Hit
CAATTCGTTGACATACTTTCCAATCAATAGGCCTGTTTACAGGAAGTTTC9270.1866967959446314No Hit
ATATGTTCCTGTGGCAGTGTGCCCCAACTCCCAATTACATATCCCATGAA8640.17400866418140404No Hit
TTGTTCACATCTATCAACAATGTCCTCCTGCAAATGAATATCAGGAAAAG8140.1639387183375728No Hit
CATTTAAACCCTCACAAAACAAAGAGATGGGGATATTCCCTTAACTTCAT7830.1576953519143974No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC7470.1504449909068389No Hit
GTACCGAACATGGAGAACATCGCATCAGGACTCCTAGGACCCCTGCTCGT7390.1488337995718259No Hit
GGAATATGATAAAACGCCGCAGACACATCCAGCGATAACCAGGACAAATT7370.14843100173807267No Hit
ATCATCTTCCTCTGCATCCTGCTGCTATGCCTCATCTTCTTGTTGGTTCT7180.14460442231741677No Hit
CCTCTATGTTTCCCTCATGTTGCTGTACAAAACCTACGGACGGAAACTGC7060.14218763531489728No Hit
CCCTTACAGTAAATGAAAAAAGAAGACTAAAATTAATTATGCCTGCTAGG6830.1375554602267349No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA6790.1367498645592284No Hit
TCCACAGAGTGTGTAAATAATGTCTCGTCTGGAAGTAATGATCAACTACA6740.13574286997484528No Hit
ATATAAAAGAGAGTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATT6640.13372888080607903No Hit
GTTCTTCTTCTAGGGGTCCTGCCTCGTTGTCTAACAACAGTAGTTTCCGG6410.12909670571791665No Hit
GATAAGATAGGGGCATTTGGAGGTCTATATGCAGGAGGAGTGCGAATCCA6390.1286939078841634No Hit
ATCCCGCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAGGCTTTT6310.1270827165491504No Hit
GTCCGGTGCTGGTTGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC6240.12567292413101402No Hit
CTGTTTACGTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAAAAGCCTGTT6140.12365893496224777No Hit
GAATTAGTAGTGGGCTATGTCAACGTTAATATGGGCATAAAACTCAGACA6110.1230547382116179No Hit
CTTTTGTATAAGATACCCGCCTTCCACAGAGTGTGTAAATAATGTCTCGT6050.12184634471035816No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAATTACTCTCTTTTTTGCCGTCTG6050.12184634471035816No Hit
GCCTAGTCATCTCTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCC5990.1206379512090984No Hit
GAGTATCCCTTAGAGGTGGAGATAAGGGAGTTGGCTGCCTTCCTGACTGC5760.11600577612093602No Hit
CTACTAATTCCCTGGATGCTGGATCTTCCAAATTACTTCCCACCCAGGTG5640.11358898911841653No Hit
GTGGATATCCCGCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAG5520.11117220211589703No Hit
GGCCTATGGCCAAGCCCCAACCAGTGGGGGTTGCGTCAGCAAACACTTGG5390.1085540161965009No Hit
ATTTCGTAACATTGGGATAAAACCTAGCAGGCATAATTAATTTTAGTCTT5380.10835261727962427No Hit
GGATAATAAGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACATT5360.10794981944587101No Hit
CCTAACTGGCAAACTCCTTCTTTTCCTGATATTCATTTGCAGGAGGACAT5300.10674142594461127No Hit
CTTTGGGACATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGA5280.10633862811085801No Hit
ACGTCAACAGTATCTGAACCTTTACCCCGTTGCTCGGCAACGGTCTGGCC5280.10633862811085801No Hit
CATCATTTCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGG5240.10553303244335152No Hit
GCCATTTAGGATTGAAGCAAGGTACCGTAGAAGAATAAAGCCCCGTAAAG5220.10513023460959826No Hit
TCATCTTCCTCTGCATCCTGCTGCTATGCCTCATCTTCTTGTTGGTTCTT5200.10472743677584502No Hit
GCCTAATCATCTCTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCC5100.10271344760707876No Hit
GCACAACTCCTGCTCAAGGCACCTCTATGTTTCCCTCATGTTGCTGTACA5050.10170645302269564No Hit
CCTCCTGCATATAGACCTCCAAATGCCCCTATCTTATCAACACTTCCGGA5040.10150505410581902No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)