FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488184

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488184
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences476860
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATGTGTTCAGG23220.48693536887136685No Hit
GTTAATATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTC16350.34286792769366276No Hit
GGATATTATCAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAACG15090.31644507822002266No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG13600.2851990101916705No Hit
GTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTC12900.27051964937298156No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN12820.26884200813656No Hit
GTGTAAAGGGAGCGGCAAAGCCCAGAAGCCCCACAATTCGCTGACATACT12670.2656964308182695No Hit
CTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGATTATGCCTGCTAGG12450.2610829174181101No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC12430.26066350710900477No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA12250.2568888143270562No Hit
CATTTAAACCCTACTAAAACTAAACGTTGGGGCTACTCCCTTAACTTCAT12140.2545820576269765No Hit
CCCTTTACACAATGTGGTTACCCAGCATTAATGCCTTTGTATGCATGTAT11410.23927358134462948No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA11280.23654741433544435No Hit
ATACAACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA11010.23088537516252147No Hit
ATGTTGTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTTTACC10510.22040011743488652No Hit
GGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATATTTGGTACGGTT10300.2159963091892799No Hit
ATCCTAACCGTACCAAATATTTGCCCTTAGATAAAGGCATTAAACCTTAT9850.2065595772344084No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA9420.19754225558864238No Hit
ACAATATGTAGGCCCTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGA9040.18957345971563982No Hit
ATATGTTCCTGTGGTAAGGTACCCCAACTTCCAATTACATATCCCATGAA8970.18810552363377092No Hit
GGTTAGGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTTTTTTCG8910.18684729270645473No Hit
ATTTATAGGAAGTTTCCGAAAACATTGTTTGATTTTTTGTACAATATGTT8910.18684729270645473No Hit
ATGTAGGCCCTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGATTATG8810.18475024116092773No Hit
GAAAAAAGGGAGTTTGCCAGTCAGGATTAAAGACAGGTACAGTAGAAGAA8260.17321645766052932No Hit
GTAGTAAGCTATGTCAATGTTAATATGGGCCTAAAAATCAGACAACTATT8010.16797382879671183No Hit
CCATATTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGGGA7980.16734471333305373No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGATC7920.16608648240573753No Hit
CCCTCTTTTCCTCACATTCATTTGAAAGAGGATATTATCAATAGATGTCA7920.16608648240573753No Hit
GGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTTTTTTCGTTAAC7760.16273119993289437No Hit
CATGTATACAAGCTAAACAGGCTTTCACTTTCTCGCCAACTTACAAGGCC7390.1549721092144445No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTACCACAG7330.1537138782871283No Hit
ATGTAAGACCTTGGGCAAGACCTGTCGGGCGTTCACGGTGGTCTCCATGC7310.1532944679780229No Hit
GCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT7180.15056830096883783No Hit
CCTCCCCCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA7140.14972948035062703No Hit
GTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATATTTGGTA7090.1486809545778635No Hit
CTTTAGGACATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGA7070.14826154426875812No Hit
GTTCTATCCTAACCGTACCAAATATTTGCCCTTAGATAAAGGCATTAAAC6950.14574508241412573No Hit
GGATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGA6870.14406744117770415No Hit
GTTCCGGAACAGTAAACCCTGTTCCGACTACTGTCTCTCCCATATCGTCA6820.14301891540494066No Hit
ATTGACAACAGTACCAGCAGCGCCTCCTCCTGCCTCCACCAATCGGCAGT6810.14280921025038795No Hit
CTCCTATACAAAACACCAGCCTTCCACAAAGTATGTAAATAATGCCTAGT6770.14197038963217717No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA6760.14176068447762447No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAG6720.14092186385941366No Hit
ATTTACATACTTTGTGGAAGGCTGGTGTTTTGTATAGGAGAGAAACTACT6710.14071215870486098No Hit
GGTCTATTTATAGGAAGTTTCCGAAAACATTGTTTGATTTTTTGTACAAT6640.13924422262299208No Hit
CTGTAAGAGGGCCTACATATTGTTGACATCTATTGATAATATCCTCTTTC6640.13924422262299208No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC6540.1371471710774651No Hit
GATAAAACGCCGCAGACACATCCAGCGATAACCAGGACAAATTGGAGGAC6490.1360986453047016No Hit
ATACAGCACTAAGGCAAGCTATTCTGTGCTGGGGTGAGTTGATGAATCTG6290.1319045422136476No Hit
GCGTTCTATCATCTTCCTCTTCATCCTGCTGCTATGCCTCATCTTCTTGT6260.13127542674998952No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC6210.13022690097722603No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT6100.12792014427714635No Hit
GTATACATGCATACAAAGGCATTAATGCTGGGTAACCACATTGTGTAAAG6060.12708132365893554No Hit
GGGTAAAGGTTCAGATATTGTTTATACAAAAAGGCCTTGTAAGTTGGCGA6040.12666191334983015No Hit
GTTTGGAAGTAATGATTAACTATGTGTTCAGGATAATAAGGTTTAATGCC6020.12624250304072474No Hit
TATCAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAACGAAAAAA5800.12162898964056537No Hit
TCCACAAAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATG5780.12120957933145995No Hit
CCTATAAATAGACCTATTGATTGGAAAGTATGTCAGCGAATTGTGGGGCT5740.12037075871324918No Hit
CCCTTTTTTCCTCACATTCATTTGAAAGAGGATATTATCAATAGATGTCA5700.11953193809503837No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAATTGC5690.11932223294048568No Hit
GTATAGGAGAGAAACTACTCACAGCGCCTCATTCTGTGGGTCACCATATT5680.11911252778593298No Hit
CAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAACGAAAAAAGGA5620.1178542968586168No Hit
GATTAACTATGTGTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGGGC5610.11764459170406408No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCACACCATACAG5590.11722518139495867No Hit
CTGTAAGGGGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATACTC5460.1144990143857736No Hit
CCCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGAAACTA5340.1119825525311412No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGATTGCCGTG5280.11072432160382502No Hit
ATCTAAGGGCAAATATTTGGTACGGTTAGGATAGAACCTAGCAGGCATAA5240.10988550098561423No Hit
AGTTAAGGGAGTAGCCCCAACGTTTAGTTTTAGTAGGGTTTAAATGTATA5210.10925638552195613No Hit
CCGAACATGGAGAGCACCACATCAGGATTCCTAGGACCCCTGCTCGTGTT5130.10757874428553454No Hit
GGCCTACATATTGTTGACATCTATTGATAATATCCTCTTTCAAATGAATG5090.10673992366732374No Hit
GTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCCTG5050.10590110304911295No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCCCCTTACAGACCACCAAATGCC5050.10590110304911295No Hit
CTCAAGAACAGTTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA5050.10590110304911295No Hit
GTGGTAAGGTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAG5000.10485257727634945No Hit
CTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGAAGAGAAACTGTTCT4940.10359434634903326No Hit
GGTTTAATGCCTTTATCTAAGGGCAAATATTTGGTACGGTTAGGATAGAA4940.10359434634903326No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC4900.10275552573082247No Hit
GCACTAAGGCAAGCTATTCTGTGCTGGGGTGAGTTGATGAATCTGGCCAC4850.10170699995805897No Hit
TTATTATCCTGAACACATAGTTAATCATTACTTCCAAACTAGGCATTATT4840.10149729480350626No Hit
GGATATTAACATTGAGGTTCCCGAGATTGAGATCTTCTGCGACGCGGCGA4830.10128758964895357No Hit
GTTTAATGCCTTTATCTAAGGGCAAATATTTGGTACGGTTAGGATAGAAC4800.10065847418529547No Hit
GGAACATATTGTACAAAAAATCAAACAATGTTTTCGGAAACTTCCTATAA4790.10044876903074279No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)