FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488185

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488185
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences476860
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATGTGTTCAGG24940.5230046554544311No Hit
GGATATTATCAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAACG15250.3198003606928658No Hit
GTTAATATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTC14980.314138321519943No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG13580.2847795998825651No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN12850.26947112360021813No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC12830.2690517132911127No Hit
GTGTAAAGGGAGCGGCAAAGCCCAGAAGCCCCACAATTCGCTGACATACT12730.2669546617455857No Hit
GTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTC12320.25835675040892503No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA11850.24850060814494818No Hit
GGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATATTTGGTACGGTT11660.2445162102084469No Hit
CATTTAAACCCTACTAAAACTAAACGTTGGGGCTACTCCCTTAACTTCAT11600.24325797928113074No Hit
CTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGATTATGCCTGCTAGG11560.2424191586629199No Hit
CCCTTTACACAATGTGGTTACCCAGCATTAATGCCTTTGTATGCATGTAT11280.23654741433544435No Hit
ATCCTAACCGTACCAAATATTTGCCCTTAGATAAAGGCATTAAACCTTAT10320.21641571949838528No Hit
ATACAACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA10290.21578660403472716No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA10180.21347984733464748No Hit
ATTTATAGGAAGTTTCCGAAAACATTGTTTGATTTTTTGTACAATATGTT10150.2128507318709894No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA9380.19670343497043158No Hit
ATGTTGTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTTTACC9310.19523549888856268No Hit
ATATGTTCCTGTGGTAAGGTACCCCAACTTCCAATTACATATCCCATGAA9050.1897831648701925No Hit
GGTTAGGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTTTTTTCG8890.1864278823973493No Hit
GAAAAAAGGGAGTTTGCCAGTCAGGATTAAAGACAGGTACAGTAGAAGAA8860.1857987669336912No Hit
ACAATATGTAGGCCCTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGA8810.18475024116092773No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGATC8700.18244348446084804No Hit
ATGTAGGCCCTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGATTATG8650.18139495868808456No Hit
GTAGTAAGCTATGTCAATGTTAATATGGGCCTAAAAATCAGACAACTATT7680.16105355869647275No Hit
CCTCCCCCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA7530.15790798137818227No Hit
GGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTTTTTTCGTTAAC7500.15727886591452417No Hit
CCATATTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGGGA7380.1547624040598918No Hit
GGATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGA7280.1526653525143648No Hit
CTTTAGGACATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGA7190.1507780061233905No Hit
ATTTACATACTTTGTGGAAGGCTGGTGTTTTGTATAGGAGAGAAACTACT7160.15014889065973241No Hit
GTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATATTTGGTA7140.14972948035062703No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTACCACAG7140.14972948035062703No Hit
CATGTATACAAGCTAAACAGGCTTTCACTTTCTCGCCAACTTACAAGGCC7120.14931007004152164No Hit
CTGTAAGAGGGCCTACATATTGTTGACATCTATTGATAATATCCTCTTTC7100.14889065973241622No Hit
CCCTCTTTTCCTCACATTCATTTGAAAGAGGATATTATCAATAGATGTCA7090.1486809545778635No Hit
GTTCCGGAACAGTAAACCCTGTTCCGACTACTGTCTCTCCCATATCGTCA7050.14784213395965273No Hit
GTTCTATCCTAACCGTACCAAATATTTGCCCTTAGATAAAGGCATTAAAC6980.14637419787778386No Hit
GGTCTATTTATAGGAAGTTTCCGAAAACATTGTTTGATTTTTTGTACAAT6930.14532567210502034No Hit
GTTTGGAAGTAATGATTAACTATGTGTTCAGGATAATAAGGTTTAATGCC6910.14490626179591495No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA6860.14385773602315144No Hit
CTCCTATACAAAACACCAGCCTTCCACAAAGTATGTAAATAATGCCTAGT6700.14050245355030827No Hit
GCGTTCTATCATCTTCCTCTTCATCCTGCTGCTATGCCTCATCTTCTTGT6590.13819569685022856No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAG6500.1363083504592543No Hit
GTATACATGCATACAAAGGCATTAATGCTGGGTAACCACATTGTGTAAAG6390.1340015937591746No Hit
GATTAACTATGTGTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGGGC6360.1333724782955165No Hit
ATTGACAACAGTACCAGCAGCGCCTCCTCCTGCCTCCACCAATCGGCAGT6160.12917837520446251No Hit
TATCAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAACGAAAAAA6150.12896867004990983No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC6150.12896867004990983No Hit
GATAAAACGCCGCAGACACATCCAGCGATAACCAGGACAAATTGGAGGAC6140.12875896489535713No Hit
GGGTAAAGGTTCAGATATTGTTTATACAAAAAGGCCTTGTAAGTTGGCGA6120.12833955458625174No Hit
CAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAACGAAAAAAGGA6110.12812984943169903No Hit
ATGTAAGACCTTGGGCAAGACCTGTCGGGCGTTCACGGTGGTCTCCATGC6060.12708132365893554No Hit
ATACAGCACTAAGGCAAGCTATTCTGTGCTGGGGTGAGTTGATGAATCTG5910.12393574634064505No Hit
GCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT5810.12183869479511805No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT5750.12058046386780187No Hit
CTCAAGAACAGTTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA5700.11953193809503837No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCACACCATACAG5650.11848341232227488No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC5640.11827370716772218No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAATTGC5600.1174348865495114No Hit
GTATAGGAGAGAAACTACTCACAGCGCCTCATTCTGTGGGTCACCATATT5490.1151281298494317No Hit
TTATTATCCTGAACACATAGTTAATCATTACTTCCAAACTAGGCATTATT5490.1151281298494317No Hit
CCCTTTTTTCCTCACATTCATTTGAAAGAGGATATTATCAATAGATGTCA5490.1151281298494317No Hit
AGTTAAGGGAGTAGCCCCAACGTTTAGTTTTAGTAGGGTTTAAATGTATA5350.11219225768569392No Hit
CTGTAAGGGGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATACTC5330.11177284737658852No Hit
GTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCCTG5270.11051461644927232No Hit
CTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGAAGAGAAACTGTTCT5210.10925638552195613No Hit
CCTTTACCCCGTTGCTCGGCAACGGTCAGGTCTTTGCCAAGTGTTTGCTG5210.10925638552195613No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGATTGCCGTG5190.10883697521285073No Hit
GGTTTAATGCCTTTATCTAAGGGCAAATATTTGGTACGGTTAGGATAGAA5150.10799815459463995No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTCTA5140.10778844944008724No Hit
TCCACAAAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATG5120.10736903913098184No Hit
CCTATAAATAGACCTATTGATTGGAAAGTATGTCAGCGAATTGTGGGGCT5100.10694962882187645No Hit
CCCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGAAACTA5100.10694962882187645No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCCCCTTACAGACCACCAAATGCC5060.10611080820366564No Hit
GGCCTACATATTGTTGACATCTATTGATAATATCCTCTTTCAAATGAATG5040.10569139789456025No Hit
ACATATTGTTGACATCTATTGATAATATCCTCTTTCAAATGAATGTGAGG5020.10527198758545485No Hit
ATCTAAGGGCAAATATTTGGTACGGTTAGGATAGAACCTAGCAGGCATAA4870.10212641026716436No Hit
GTGGTAAGGTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAG4840.10149729480350626No Hit
GAAAAGAGGGAGTTTGCCAGTCAGGATTAAAGACAGGTACAGTAGAAGAA4820.10107788449440087No Hit
GTTTAATGCCTTTATCTAAGGGCAAATATTTGGTACGGTTAGGATAGAAC4810.10086817933984817No Hit
CTCCTACCTGGTGTGCCGCAGGCCATTGATCCTTGTTGGGGTTGAAGTCC4800.10065847418529547No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)