FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488186

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488186
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences535180
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN28870.5394446728203596No Hit
TCCAAAGAGTGTGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACA19180.35838409507081725No Hit
ACTTACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT15120.28252176837699466No Hit
GTGTAGACTCTCTTTTATATAAGATACCCGCCTTCCAAAGAGTGTGTAAA13600.25412010912216454No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA13510.2524384319294443No Hit
GGATAATAAGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACATT13080.24440375200866998No Hit
GTAAACGAAAAAAGGAGACTAAAATTAATTATGCCTGCTAGGTTTTATCC12820.2395455734519227No Hit
CTTTTATATAAGATACCCGCCTTCCAAAGAGTGTGTAAATAATGTCTCGT12330.23038977540266828No Hit
ATGTGCGGACTCTCTTTTATATAAGATACCCGCCTTCCAAAGAGTGTGTA12060.22534474382450764No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC11940.22310250756754735No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGA11660.21787062296797338No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAGTTTTCTAGGGGGAACACCCGTG11510.215067827646773No Hit
TTATTATCCAGAGCATGTAGTTAATCATTACTTCCAGACGAGACATTATT11360.2122650323255727No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT9980.18647931537052956No Hit
GTTGTAAGTATGCCCTGAGCCTGAGGGCTCCACCCCAACAGTCCCCCATG9560.17863148847116858No Hit
CCCTTACAGTAAATGAAAAAAGGAGACTAAAATTAATTATGCCTGCTAGG8920.16667289510071379No Hit
CGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCCT8510.15901192122276617No Hit
ATATGGAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA8340.1558354198587391No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACGAGAAAAACCCC8240.15396688964460556No Hit
GTTGATAGATGTACTCAATTTGTGGGGCCCCTTACAGTAAATGAAAAAAG8200.15321947755895213No Hit
TCCCGATACAGAGCAGAGGCGGTGTCAAGGAGATCTCGAATAGAAGGAAA7920.14798759295937816No Hit
CCTTATGTGTCCAAGGAATACTAACATTGAGATCCCCGAGATTGAGATCT7510.14032661908143057No Hit
GTTTTATCCCAATGTTACTAAATATTTGCCCTTAGATAAAGGGATCAAAC7500.14013976606001718No Hit
TCTATCAACAATGTCCTCCTGCAAGTGAATGTCAGGAAAAGAAGGAGTTT7480.13976606001719047No Hit
GTGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACATGCTCTGGAT7420.13864494188871035No Hit
GTCCGGTGCTGGTTGTTGACGATCCTGGAATTAGAGGACAAACGGGCAAC7350.13733697073881684No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAA7120.13303935124630964No Hit
CATGTATACAAGCGAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC7000.13079711498934937No Hit
CTCTAAAGCCTCCCGATACAGAGCAGAGGCGGTGTCAAGGAGATCTCGAA6980.13042340894652268No Hit
ATATGTTCCTGTGGCAGTGTGCCCCAACTCCCAATTACATATCCCATGAA6970.1302365559251093No Hit
CCTCTATGTTTCCCTCATGCTGCTGTACAAAACCTACGGACGGAAACTGC6890.12874173175380246No Hit
TAGATAAAGGGATCAAACCTTATTATCCAGAGCATGTAGTTAATCATTAC6820.12743376060390899No Hit
CACATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACGGTACCTTGC6770.12649949549684217No Hit
ATCCCAATGTTACTAAATATTTGCCCTTAGATAAAGGGATCAAACCTTAT6730.12575208341118876No Hit
GCTCTGGATAATAAGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTA6480.12108075787585486No Hit
CTGTAAGGGGCCCCACAAATTGAGTACATCTATCAACAATGTCCTCCTGC6450.12052019881161478No Hit
ATAATGTCTCGTCTGGAAGTAATGATTAACTACATGCTCTGGATAATAAG6240.1165962853619343No Hit
CTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGGAGAGAAACTGTTCT6080.1136066370193206No Hit
ATCCAGCATCCAGGGAAGCAGTAGTCAGCTATGTCAACGTTAATATGGGC6060.11323293097649388No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCGAAACAG5990.11192495982660039No Hit
GCCTAGCAGCCATGGAAATGATGTATACTTGCGGGAGAGGACGACAGAAT5960.11136440076236033No Hit
TTTTACGGGTCACCATATTCTTGGGAACAAGATCTACAGCATGGGAGGTT5940.11099069471953361No Hit
CCCTTAGATAAAGGGATCAAACCTTATTATCCAGAGCATGTAGTTAATCA5930.11080384169812026No Hit
GTGTGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACATGCTCTGG5930.11080384169812026No Hit
GTTTACAGGAAGTTTTCTAAAACACATTTTGATTTTGTGTACAATATGTT5920.1106169886767069No Hit
GTCTATATGGAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATAT5890.11005642961246682No Hit
GGAACATATTGTACACAAAATCAAAATGTGTTTTAGAAAACTTCCTGTAA5890.11005642961246682No Hit
ATACAGAGCAGAGGCGGTGTCAAGGAGATCTCGAATAGAAGGAAAGAAGT5840.10912216450540005No Hit
ATATAGACCTCCAAATGCCCCTATCTTATCAACACTTCCGGAAACTACTG5750.10744048731267984No Hit
CACACAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACGGTACCTTGC5740.10725363429126648No Hit
GGTCTATATGGAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATA5710.10669307522702644No Hit
ATATAAGATACCCGCCTTCCAAAGAGTGTGTAAATAATGTCTCGTCTGGA5670.10594566314137299No Hit
ATGTGTAGACTCTCTTTTATATAAGATACCCGCCTTCCAAAGAGTGTGTA5670.10594566314137299No Hit
GTATCGGGAGGCTTTAGAGTCTCCGGAACATTGTTCAGCTCACCATACGG5610.10482454501289287No Hit
ATATTTGCCCTTAGATAAAGGGATCAAACCTTATTATCCAGAGCATGTAG5570.10407713292723943No Hit
GTCAAGGAGATCTCGAATAGAAGGAAAGAAGTCAGACGGCAAAAAAGAGA5540.10351657386299935No Hit
GGCAGAGACAGTATTCTGAGCAGGGCTCACTGTTCCTGAACTGGAGCCAC5370.1003400724989723No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)