FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488187

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488187
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences535180
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN28710.5364550244777457No Hit
TCCAAAGAGTGTGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACA18650.3484808849359094No Hit
GGATAATAAGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACATT14670.2741133824133936No Hit
GTAAACGAAAAAAGGAGACTAAAATTAATTATGCCTGCTAGGTTTTATCC13980.2612205239358721No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA13930.26028625882880524No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAGTTTTCTAGGGGGAACACCCGTG13270.24795395941552373No Hit
ACTTACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT13140.2455248701371501No Hit
GTGTAGACTCTCTTTTATATAAGATACCCGCCTTCCAAAGAGTGTGTAAA13090.2445906050300833No Hit
TTATTATCCAGAGCATGTAGTTAATCATTACTTCCAGACGAGACATTATT12130.22665271497440115No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC12110.22627900893157443No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGA11700.2186180350536268No Hit
ATGTGCGGACTCTCTTTTATATAAGATACCCGCCTTCCAAAGAGTGTGTA11630.21731006390373334No Hit
CTTTTATATAAGATACCCGCCTTCCAAAGAGTGTGTAAATAATGTCTCGT11550.21581523973242647No Hit
GTTGTAAGTATGCCCTGAGCCTGAGGGCTCCACCCCAACAGTCCCCCATG9450.17657610523562167No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT9290.17358645689300797No Hit
GTATAAAGAATTTGGAGCTTCTGCGGAGTTACTCTCTTTTTTGCCGTCTG8880.16592548301506035No Hit
GTTTTATCCCAATGTTACTAAATATTTGCCCTTAGATAAAGGGATCAAAC8850.16536492395082028No Hit
CGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCCT8410.15714339100863262No Hit
ATATGGAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA8350.15602227288015247No Hit
CCCTTACAGTAAATGAAAAAAGGAGACTAAAATTAATTATGCCTGCTAGG8320.1554617138159124No Hit
GTTGATAGATGTACTCAATTTGTGGGGCCCCTTACAGTAAATGAAAAAAG7980.14910871108785828No Hit
GTCCGGTGCTGGTTGTTGACGATCCTGGAATTAGAGGACAAACGGGCAAC7740.14462423857393775No Hit
CCTTATGTGTCCAAGGAATACTAACATTGAGATCCCCGAGATTGAGATCT7690.14368997346687096No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACGAGAAAAACCCC7650.14294256138121753No Hit
TCTATCAACAATGTCCTCCTGCAAGTGAATGTCAGGAAAAGAAGGAGTTT7480.13976606001719047No Hit
GTGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACATGCTCTGGAT7370.13771067678164356No Hit
CCTCTATGTTTCCCTCATGCTGCTGTACAAAACCTACGGACGGAAACTGC7370.13771067678164356No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAA7360.1375238237602302No Hit
ATCCCAATGTTACTAAATATTTGCCCTTAGATAAAGGGATCAAACCTTAT6810.12724690758249563No Hit
CTTTAGGACATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGCGGA6800.12706005456108227No Hit
ATATGTTCCTGTGGCAGTGTGCCCCAACTCCCAATTACATATCCCATGAA6430.12014649276878807No Hit
GTTTACAGGAAGTTTTCTAAAACACATTTTGATTTTGTGTACAATATGTT6420.11995963974737471No Hit
CATGTATACAAGCGAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC6320.11809110953324115No Hit
GTGTGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACATGCTCTGG6170.1152883142120408No Hit
GTATCGGGAGGCTTTAGAGTCTCCGGAACATTGTTCAGCTCACCATACGG6160.11510146119062745No Hit
CACATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACGGTACCTTGC6140.11472775514780074No Hit
TAGATAAAGGGATCAAACCTTATTATCCAGAGCATGTAGTTAATCATTAC6070.11341978399790724No Hit
GGTCTATATGGAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATA6070.11341978399790724No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCGAAACAG6060.11323293097649388No Hit
GCTCTGGATAATAAGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTA5960.11136440076236033No Hit
CTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGGAGAGAAACTGTTCT5940.11099069471953361No Hit
GCCTAGCAGCCATGGAAATGATGTATACTTGCGGGAGAGGACGACAGAAT5900.11024328263388018No Hit
GTCTATATGGAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATAT5800.10837475241974662No Hit
GGAACATATTGTACACAAAATCAAAATGTGTTTTAGAAAACTTCCTGTAA5780.10800104637691992No Hit
CTGTAAGGGGCCCCACAAATTGAGTACATCTATCAACAATGTCCTCCTGC5760.1076273403340932No Hit
ATAATGTCTCGTCTGGAAGTAATGATTAACTACATGCTCTGGATAATAAG5710.10669307522702644No Hit
ATGTGTAGACTCTCTTTTATATAAGATACCCGCCTTCCAAAGAGTGTGTA5560.10389027990582607No Hit
CCCTTATCTCCACCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAA5550.10370342688441271No Hit
ATCTAAGGGCAAATATTTAGTAACATTGGGATAAAACCTAGCAGGCATAA5470.10220860271310586No Hit
ATATTTGCCCTTAGATAAAGGGATCAAACCTTATTATCCAGAGCATGTAG5440.10164804364886582No Hit
ACATTGACCCGTATAAAGAATTTGGAGCTTCTGCGGAGTTACTCTCTTTT5380.10052692552038565No Hit
GCTTTAGGACATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGCGG5370.1003400724989723No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)