FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488188

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488188
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences504982
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN21570.42714393780372373No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA15400.3049613649595431No Hit
GTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATATGTTCAGG15330.30357517693699976No Hit
GTGTAAAGGGAGCGGCAAAACCCAAAAGACCCACAATTCTTTGACATACT13770.2726829867203187No Hit
CCCTTTACACAATGTGGATACCCAGCATTAATGCCTTTGTATGCATGTAT12670.2509000320803514No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGATC11280.22337429848984716No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG10420.20634398849859997No Hit
CTCTTACAGTTAACGAAAAAAGAAGATTAAAATTGATCATGCCTGCTAGG9680.1916900008317128No Hit
GGATATTATCAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAACG9230.18277879211536255No Hit
ATGTAAGACCTTGGGCAAGACCTGGCGGGCGTTCACGGTGGTCTCCATGC8790.17406561025937559No Hit
CTACTGTACCTGTCTTTAATCCTGAATGGCAAACTCCCTCTTTTCCACAC8780.17386758339901226No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA8550.16931296561065542No Hit
CTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG8450.16733269700702202No Hit
GTTTAGGATAGAACCTAGCAGGCATGATCAATTTTAATCTTCTTTTTTCG8170.16178794491684853No Hit
GTATACATGCATACAAAGGCATTAATGCTGGGTATCCACATTGTGTAAAG8130.16099583747539517No Hit
ATCCTAAACTTACTAAATATTTGCCCTTAGATAAAGGCATCAAACCTTAT8070.1598076763132151No Hit
ATACCTTCCTGCTGGTGGCTCCAGTTCCGGAACAGTAAACCCTGTTCCGA7700.15248068247977156No Hit
ATATGTTCCTGTGGCAAGGTACCCCAACTTCCAATTACATATCCCATGAA7670.15188660189868153No Hit
CATTTAAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT7510.1487181721328681No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG7320.14495566178596464No Hit
GGATAATAAGGTTTGATGCCTTTATCTAAGGGCAAATATTTAGTAAGTTT7250.14356947376342127No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTGATAATATCCTCTTTT7220.14297539318233124No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACGAGAAAAACCCC7190.14238131260124123No Hit
ATTAACAACAGTACCAGCAGCGCCTCCTCCTGCCTCCACCAATCGGCAGT7140.14139117829942452No Hit
CTCCATATTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGG7040.13941090969579115No Hit
GCACTAAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCAC7030.1392128828354278No Hit
GCCTACTACTAATTCCCTGGATGCCGGGTCTTCCAAATTACTTCCCACCC7010.1388168291147011No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTGCCACAG6930.1372326142317944No Hit
CTCTCAGAGGTGGAGAGATGGGAGTAGGCTTTCTTCCTGACTGCCGATTG6930.1372326142317944No Hit
CCTATAAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCT6920.13703458737143107No Hit
ATTTACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACT6900.13663853365070436No Hit
GTAGTAGGCTATGTCAATGTTAATATGGGCCTAAAAATCAGACAAATATT6790.13446023818670766No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC6710.13287602330380094No Hit
GCCTAGCAGCCATGGAAAAGAGGTGTATTTCCGAGAGAGGACAACAGAGT6350.1257470563307207No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG6220.12317270714599729No Hit
GTTCAGGATAATAAGGTTTGATGCCTTTATCTAAGGGCAAATATTTAGTA6170.12218257284418058No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC6170.12218257284418058No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA6110.12099441168200054No Hit
ATTTATAGGAAGTTTCCGAAAACATTGTTTGATTTTTTGTACAATATGTT6030.11941019679909382No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGAATGGCAAACTCCCTCTTTTC6020.1192121699387305No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC5970.11822203563691379No Hit
CCCAAAGACAAAAGAAAATTGGTAATAGCGGTAAAAAGGGATTCAAGATG5790.11465755215037368No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCCGCTTACAGACCACCAAATGCC5510.10911280006020017No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAG5440.10772661203765678No Hit
TCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCACGAATAGAAGGAAA5350.10594437029438673No Hit
CTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT5330.10554831657366005No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACCTGGCGGGCGTTC5290.1047562091322067No Hit
GGAACATATTGTACAAAAAATCAAACAATGTTTTCGGAAACTTCCTATAA5290.1047562091322067No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG5250.10396410169075333No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATA5240.10376607483038999No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC5200.10297396738893665No Hit
GTTGTTAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGATTGCCGTG5190.10277594052857329No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT5130.10158777936639327No Hit
AGTCTGTACAACATCTTGAATCCCTTTTTACCGCTATTACCAATTTTCTT5130.10158777936639327No Hit
GGATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGTGA5130.10158777936639327No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG5100.10099369878530323No Hit
TCACCATACAGCACTAAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGA5100.10099369878530323No Hit
GTTCTATCCTAAACTTACTAAATATTTGCCCTTAGATAAAGGCATCAAAC5080.10059764506457655No Hit
CTATATGTTCAGGATAATAAGGTTTGATGCCTTTATCTAAGGGCAAATAT5070.10039961820421323No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTAAGGCA5050.10000356448348655No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)