FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488189

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488189
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences504982
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN21530.4263518303622703No Hit
GTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATATGTTCAGG17430.3451608176133011No Hit
GTGTAAAGGGAGCGGCAAAACCCAAAAGACCCACAATTCTTTGACATACT13680.2709007449770487No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA13420.2657520466076019No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG11730.23228550720619745No Hit
CCCTTTACACAATGTGGATACCCAGCATTAATGCCTTTGTATGCATGTAT11560.22891905058002068No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGATC10880.21545322407531356No Hit
CTCTTACAGTTAACGAAAAAAGAAGATTAAAATTGATCATGCCTGCTAGG9770.19347224257498286No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA9420.186541302462266No Hit
CTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG8870.1756498251422823No Hit
GGATATTATCAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAACG8850.17525377142155563No Hit
GTTTAGGATAGAACCTAGCAGGCATGATCAATTTTAATCTTCTTTTTTCG8530.16891691188992874No Hit
GTATACATGCATACAAAGGCATTAATGCTGGGTATCCACATTGTGTAAAG8220.16277807921866522No Hit
CTCCATATTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGG7810.1546589779437683No Hit
CTACTGTACCTGTCTTTAATCCTGAATGGCAAACTCCCTCTTTTCCACAC7370.14594579608778135No Hit
CCTATAAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCT7350.14554974236705465No Hit
GGATAATAAGGTTTGATGCCTTTATCTAAGGGCAAATATTTAGTAAGTTT7230.1431734200426946No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACGAGAAAAACCCC7220.14297539318233124No Hit
ATACCTTCCTGCTGGTGGCTCCAGTTCCGGAACAGTAAACCCTGTTCCGA7150.14158920515978787No Hit
ATATGTTCCTGTGGCAAGGTACCCCAACTTCCAATTACATATCCCATGAA7130.1411931514390612No Hit
CATTTAAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT7030.1392128828354278No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG7020.13901485597506447No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTGATAATATCCTCTTTT7010.1388168291147011No Hit
ATCCTAAACTTACTAAATATTTGCCCTTAGATAAAGGCATCAAACCTTAT6990.13842077539397443No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTGCCACAG6940.13743064109215775No Hit
GCCTACTACTAATTCCCTGGATGCCGGGTCTTCCAAATTACTTCCCACCC6670.13208391586234758No Hit
ATTTACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACT6650.1316878621416209No Hit
ATTAACAACAGTACCAGCAGCGCCTCCTCCTGCCTCCACCAATCGGCAGT6640.13148983528125754No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG6620.13109378156053086No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC6480.1283214055154441No Hit
ATGTAAGACCTTGGGCAAGACCTGGCGGGCGTTCACGGTGGTCTCCATGC6390.12653916377217406No Hit
GTAGTAGGCTATGTCAATGTTAATATGGGCCTAAAAATCAGACAAATATT6330.12535100260999402No Hit
CTCTCAGAGGTGGAGAGATGGGAGTAGGCTTTCTTCCTGACTGCCGATTG6220.12317270714599729No Hit
GCCTAGCAGCCATGGAAAAGAGGTGTATTTCCGAGAGAGGACAACAGAGT6220.12317270714599729No Hit
GTTTGGGACTCTACCGTCCCCTTCTTCGTCTGCCGTTCCGACCGACCACG6180.12238059970454392No Hit
GCACTAAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCAC6100.1207963848216372No Hit
CCCAAAGACAAAAGAAAATTGGTAATAGCGGTAAAAAGGGATTCAAGATG6000.11881611621800381No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTCTA5980.11842006249727713No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA5910.11703387447473375No Hit
GTTCAGGATAATAAGGTTTGATGCCTTTATCTAAGGGCAAATATTTAGTA5880.11643979389364373No Hit
ATTTATAGGAAGTTTCCGAAAACATTGTTTGATTTTTTGTACAATATGTT5810.11505360587110035No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAG5670.11228122982601359No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGAATGGCAAACTCCCTCTTTTC5640.11168714924492358No Hit
GTCTTTGGGTATACATTTAAACCCTAATAAAACCAAACGTTGGGGCTACT5520.1093108269205635No Hit
CTTCAAAGACTGTGTATTTAAGGACTGGGAGGAGTTGGGGGAGGAGACTA5460.10812266575838346No Hit
CTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT5420.10733055831693011No Hit
GGAACATATTGTACAAAAAATCAAACAATGTTTTCGGAAACTTCCTATAA5340.1057463434340234No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTTTACC5280.10455818227184335No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTAAGGCA5210.10317199424929999No Hit
GGATAGAACCTAGCAGGCATGATCAATTTTAATCTTCTTTTTTCGTTAAC5140.1017858062267566No Hit
TATCAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAACGAAAAAA5140.1017858062267566No Hit
GTTCTATCCTAAACTTACTAAATATTTGCCCTTAGATAAAGGCATCAAAC5140.1017858062267566No Hit
CCTTTACCCCGTTGCTCGGCAACGGTCAGGTCTTTGCCAAGTGTTTGCTG5120.10138975250602993No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG5090.10079567192493989No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC5060.10020159134384989No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)