FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488192

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488192
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences443318
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN28780.6491953857050695No Hit
TCCAAAGAGTGTGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACA19160.43219539923937217No Hit
CTTTTATATAAAATACCCGCCTTCCAAAGAGTGTGTAAATAATGTCTCGT14040.31670268294993664No Hit
GGATAATAAGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACATT12440.280611209109488No Hit
CCCTTACAGTAAATGAAAACAGGAGACTAAAATTAATTATGCCTGCTAGG10530.23752701221245245No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACAGTGCTGAAAGTCCAA9450.21316526737014965No Hit
TTATTATCCAGAGCATGTAGTTAATCATTACTTCCAGACGAGACATTATT9320.2102328351206132No Hit
TTCAAGAACAGTGTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA9110.20549582917905432No Hit
CGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCCT8600.19399167189241132No Hit
GTGCTGGTGAACGCACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC8380.18902909423934963No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAGTTTTCTAGGGGGAACACCCGTG8240.1858710902783104No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC8200.18496880343229916No Hit
GTGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACATGCTCTGGAT8150.18384094487478514No Hit
GTTGATAGATGTAAGCAATTTGTGGGACCCCTTACAGTAAATGAAAACAG7410.16714863822357764No Hit
CTCTTTGGAAGGCGGGTATTTTATATAAAAGAGAGTCCACACGTAGCGCC7330.16534406453155523No Hit
CTGTAAGGGGTCCCACAAATTGCTTACATCTATCAACAATGTCCTCCTGC7330.16534406453155523No Hit
ACTAACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGAAGT6730.151809761841387No Hit
ATATTAACGTTGACATAGCTGACTACTAATTCCCTGGATGCTGGATCTTC6720.1515841901298842No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTGTCTCTTCCAAAAGTAAGA6670.15045633157237018No Hit
GATGTAAGCAATTTGTGGGACCCCTTACAGTAAATGAAAACAGGAGACTA6550.14774947103433653No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACCCCCAATTACATATCCCATGAA6390.14414032365029167No Hit
CATTTAAACCCTCACAAAACAAAACGATGGGGATATTCCCTTAACTTCAT6260.1412078914007552No Hit
ATATAAAAGAGAGTCCACACGTAGCGCCTCATTTTACGGGTCACCATATT6240.14075674797774962No Hit
GTTAGTATTCCTTGGACACATAAGGTGGGAAACTTTACGGGGCTTTATTC6180.1394033177087328No Hit
GCTTTATTCTTCTACGGTACCTTGCTTTAATCCTAAATGGCAAACTCCTT5960.1344407400556711No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG5860.13218502294064305No Hit
TCTATCAACAATGTCCTCCTGCAAATGAATGTCAGGAAAAGAAGGAGTTT5810.13105716438312903No Hit
CTTTAGGACATGGACATCGACCCATATAAAGAATTTGGAGCTTCTGTGGA5800.13083159267162622No Hit
GTGTGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACATGCTCTGG5590.12609458673006738No Hit
CCTCCTGGATATAGACCACAAAATGCCCCTATCTTATCAACACTTCCGGA5460.12316215448053089No Hit
GGAACATATTGTACAAAAAATCAAAATGTGTTTTAGGAAACTTCCTGTAA5380.12135758078850847No Hit
ATCTAAGGGCAAATATTTAGTAACATTGGGATAAAACCTAGCAGGCATAA5360.12090643736550287No Hit
TGATAGATGTAAGCAATTTGTGGGACCCCTTACAGTAAATGAAAACAGGA5280.11910186367348043No Hit
CTCTTTTATATAAAATACCCGCCTTCCAAAGAGTGTGTAAATAATGTCTC5210.1175228616929608No Hit
CCAAAAGACACCAAATATTCAAGAACAGTGTCTCTTCCAAAAGTAAGACA5080.11459042944342436No Hit
ATATAAAATACCCGCCTTCCAAAGAGTGTGTAAATAATGTCTCGTCTGGA5030.11346257088591033No Hit
GTTTTATCCCAATGTTACTAAATATTTGCCCTTAGATAAAGGGATCAAAC4960.11188356890539071No Hit
TCCTTGGACACATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACGG4950.11165799719388791No Hit
CTACGGTACCTTGCTTTAATCCTAAATGGCAAACTCCTTCTTTTCCTGAC4950.11165799719388791No Hit
GGATAAAACCTAGCAGGCATAATTAATTTTAGTCTCCTGTTTTCATTTAC4940.1114324254823851No Hit
CACATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACGGTACCTTGC4930.1112068537708823No Hit
GAATTAGTAGTCAGCTATGTCAACGTTAATATGGGCCTAAAAATCAGACA4910.11075571034787669No Hit
GTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGTACAATATGTT4880.1100789952133683No Hit
GCACCGGACCATGCAAAACCTGCACGACTCCTGCTCAAGGAACCTCTATG4850.10940228007885987No Hit
GTTCCTGAACTGGAGCCACCAGCAGGAAAGTACAGGGCCCTGACTCTGGG4760.10737213467533464No Hit
GTGCTGGGGTGAGTTGATGAATCTAGCCACCTGGGTGGGAAGTAATTTGG4720.10646984782932342No Hit
GTCCGGTGCTGGTTGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC4700.10601870440631782No Hit
ATATCCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA4670.1053419892718094No Hit
TCCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGAA4650.1048908458488038No Hit
GTTGTTAGTATGCCCTGAGCCTGAGGGCTCCACCCCAACAGTCCCCCATG4620.10421413071429537No Hit
GGTCTGTGCCAAGTGTTTGCTGACGCAACCCCCACTGGTTGGGGCTTGGC4620.10421413071429537No Hit
TAGATAAAGGGATCAAACCTTATTATCCAGAGCATGTAGTTAATCATTAC4580.10331184386828418No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTATGCC4560.10286070044527855No Hit
GTCTATATCCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATAT4500.10150727017626174No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAA4500.10150727017626174No Hit
ATGTATACTTGCGGGAGAGCACGACAGAATTGTCAGTCCCAATGAGTTTC4460.10060498333025053No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)