FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488193

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488193
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences443318
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN28750.6485186705705611No Hit
TCCAAAGAGTGTGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACA18900.4263305347402993No Hit
CTTTTATATAAAATACCCGCCTTCCAAAGAGTGTGTAAATAATGTCTCGT15140.341515571215245No Hit
GGATAATAAGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACATT13620.30722867106681884No Hit
CCCTTACAGTAAATGAAAACAGGAGACTAAAATTAATTATGCCTGCTAGG10730.24203844644250855No Hit
TTATTATCCAGAGCATGTAGTTAATCATTACTTCCAGACGAGACATTATT9600.2165488430426917No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACAGTGCTGAAAGTCCAA9170.20684925944807114No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC8750.19737524756495337No Hit
GTGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACATGCTCTGGAT8640.19489395873842252No Hit
TTCAAGAACAGTGTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA8570.1933149567579029No Hit
CGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCCT8430.19015695279686365No Hit
ATATTAACGTTGACATAGCTGACTACTAATTCCCTGGATGCTGGATCTTC8150.18384094487478514No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAGTTTTCTAGGGGGAACACCCGTG8140.18361537316328233No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTGTCTCTTCCAAAAGTAAGA7730.1743669329916674No Hit
GTTGATAGATGTAAGCAATTTGTGGGACCCCTTACAGTAAATGAAAACAG7600.17143450074213093No Hit
CCTCCTGGATATAGACCACAAAATGCCCCTATCTTATCAACACTTCCGGA6760.1524864769758954No Hit
CTCTTTGGAAGGCGGGTATTTTATATAAAAGAGAGTCCACACGTAGCGCC6700.15113304670687858No Hit
CTGTAAGGGGTCCCACAAATTGCTTACATCTATCAACAATGTCCTCCTGC6550.14774947103433653No Hit
GATGTAAGCAATTTGTGGGACCCCTTACAGTAAATGAAAACAGGAGACTA6470.1459448973423141No Hit
CATTTAAACCCTCACAAAACAAAACGATGGGGATATTCCCTTAACTTCAT6390.14414032365029167No Hit
GTGCTGGTGAACGCACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC6390.14414032365029167No Hit
ACTAACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGAAGT6340.14301246509277762No Hit
GTTAGTATTCCTTGGACACATAAGGTGGGAAACTTTACGGGGCTTTATTC5940.1339895966326655No Hit
GTTTTATCCCAATGTTACTAAATATTTGCCCTTAGATAAAGGGATCAAAC5890.13286173807515148No Hit
GCTTTATTCTTCTACGGTACCTTGCTTTAATCCTAAATGGCAAACTCCTT5860.13218502294064305No Hit
ATCTAAGGGCAAATATTTAGTAACATTGGGATAAAACCTAGCAGGCATAA5850.13195945122914027No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG5810.13105716438312903No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACCCCCAATTACATATCCCATGAA5760.12992930582561504No Hit
ACCTTATTATCCAGAGCATGTAGTTAATCATTACTTCCAGACGAGACATT5730.12925259069110662No Hit
GTGTGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACATGCTCTGG5590.12609458673006738No Hit
ATATAAAATACCCGCCTTCCAAAGAGTGTGTAAATAATGTCTCGTCTGGA5520.12451558474954771No Hit
ATATAAAAGAGAGTCCACACGTAGCGCCTCATTTTACGGGTCACCATATT5440.1227110110575253No Hit
TCTATCAACAATGTCCTCCTGCAAATGAATGTCAGGAAAAGAAGGAGTTT5320.12000415051949165No Hit
CTTTATCTAAGGGCAAATATTTAGTAACATTGGGATAAAACCTAGCAGGC5160.11639500313544678No Hit
CTTTAGGACATGGACATCGACCCATATAAAGAATTTGGAGCTTCTGTGGA5150.11616943142394398No Hit
CTCTTTTATATAAAATACCCGCCTTCCAAAGAGTGTGTAAATAATGTCTC5140.11594385971244119No Hit
CCAAAAGACACCAAATATTCAAGAACAGTGTCTCTTCCAAAAGTAAGACA5080.11459042944342436No Hit
GTTCCTGAACTGGAGCCACCAGCAGGAAAGTACAGGGCCCTGACTCTGGG5040.11368814259741314No Hit
TCCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGAA4940.1114324254823851No Hit
GGATAAAACCTAGCAGGCATAATTAATTTTAGTCTCCTGTTTTCATTTAC4930.1112068537708823No Hit
GTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGTACAATATGTT4870.10985342350186547No Hit
GTTGTTAGTATGCCCTGAGCCTGAGGGCTCCACCCCAACAGTCCCCCATG4850.10940228007885987No Hit
GCACCGGACCATGCAAAACCTGCACGACTCCTGCTCAAGGAACCTCTATG4850.10940228007885987No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTT4850.10940228007885987No Hit
GGAACATATTGTACAAAAAATCAAAATGTGTTTTAGGAAACTTCCTGTAA4830.10895113665585426No Hit
CCCTTATCTCCACCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAA4810.10849999323284865No Hit
GTCCGGTGCTGGTTGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC4730.10669541954082622No Hit
TCCTTGGACACATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACGG4720.10646984782932342No Hit
GAATTAGTAGTCAGCTATGTCAACGTTAATATGGGCCTAAAAATCAGACA4720.10646984782932342No Hit
GGTCTGTGCCAAGTGTTTGCTGACGCAACCCCCACTGGTTGGGGCTTGGC4670.1053419892718094No Hit
ATATCCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA4640.10466527413730099No Hit
TGATAGATGTAAGCAATTTGTGGGACCCCTTACAGTAAATGAAAACAGGA4630.10443970242579817No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAA4620.10421413071429537No Hit
CTACGGTACCTTGCTTTAATCCTAAATGGCAAACTCCTTCTTTTCCTGAC4580.10331184386828418No Hit
CTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGAAGAGACACTGTTCT4510.10173284188776453No Hit
GCTCTGGATAATAAGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTA4510.10173284188776453No Hit
ACTACATGCTCTGGATAATAAGGTTTGATCCCTTTATCTAAGGGCAAATA4480.10105612675325613No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)