FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488194

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488194
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences497403
Sequences flagged as poor quality0
Sequence length35-151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATATGTTCAGG17360.34901277233953154No Hit
TTATCAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAACGAAAAA17190.34559502053666746No Hit
CTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGATCATGCCTGCTAGG15270.30699452958667317No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN14620.29392665504631055No Hit
GTTTAGGATAGAACCTAGCAGGCATGATCAATTTTAATCTCCTTTTTTCG11170.22456639787053959No Hit
CCCTTTACACAATGTGGTTACCCAGCATTAATGCCTTTGTATGCATGTAT10370.20848285997470867No Hit
GTTCAGGATAGTAAGGTTTGATGCCTTTATCTAAGGGCAAATATTTGGTA9800.1970233392239291No Hit
GTGTAAAGGGAGCGGCAAAACCCAAAAGACCCACAATTCTTTGACATACT9680.19461080853955443No Hit
CAATATGTGGGCCCTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGAT9630.193605587421065No Hit
GGTAAGTTTAGGATAGAACCTAGCAGGCATGATCAATTTTAATCTCCTTT8910.17913040331481717No Hit
CTACTGTACCTGTCTTTAATCCTGAATGGCAAACTCCCTCTTTTCCTCAC8520.17128967859059957No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA8210.16505730765596507No Hit
CTATTAATAATATCCTCTTTCAAATGAATGTGAGGAAAAGAGGGAGTTTG8000.16083537895830946No Hit
GAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATATGTTCA7790.15661345026065382No Hit
GTATACATGCATACAAAGGCATTAATGCTGGGTAACCACATTGTGTAAAG7710.15500509647107075No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGATC7600.152793610010394No Hit
ATCCTAAACTTACCAAATATTTGCCCTTAGATAAAGGCATCAAACCTTAC7600.152793610010394No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGAATGGCAAACTCCCTCTTTTC7590.1525925657866961No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATA7450.14977794665492566No Hit
ATCCTAACCTTACCAAATATTTGCCCTTAGATAAAGGCATCAAACCTTAC7350.1477675044179468No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA6310.1268589051533666No Hit
CTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT5990.1204254899950342No Hit
GGATAGAACCTAGCAGGCATGATCAATTTTAATCTCCTTTTTTCGTTAAC5980.12022444577133631No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG5930.11921922465284687No Hit
ACTATATGTTCAGGATAGTAAGGTTTGATGCCTTTATCTAAGGGCAAATA5860.11781191508696168No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC5700.11459520750779549No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC5610.11278580949451451No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTTTTTCCTTCTATTCG5120.10293464253331805No Hit
CAATTTGTGGGCCCTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGAT5040.10132628874373495No Hit
CCATCTTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGGGA5030.10112524452003707No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)