FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488195

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488195
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences497403
Sequences flagged as poor quality0
Sequence length35-151
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATATGTTCAGG21310.42842524070019683No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN14520.29191621280933167No Hit
TTATCAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAACGAAAAA13140.2641721099390233No Hit
CTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGATCATGCCTGCTAGG12320.24768648359579656No Hit
GTTTAGGATAGAACCTAGCAGGCATGATCAATTTTAATCTCCTTTTTTCG11010.22134969029137339No Hit
GTTCAGGATAGTAAGGTTTGATGCCTTTATCTAAGGGCAAATATTTGGTA9600.19300245474997135No Hit
GAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATATGTTCA8120.1632479096426841No Hit
CCCTTTACACAATGTGGTTACCCAGCATTAATGCCTTTGTATGCATGTAT7700.15480405224737287No Hit
GGTAAGTTTAGGATAGAACCTAGCAGGCATGATCAATTTTAATCTCCTTT7630.15339674268148765No Hit
GTGTAAAGGGAGCGGCAAAACCCAAAAGACCCACAATTCTTTGACATACT7580.15239152156299823No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATA7360.1479685486416447No Hit
GTATACATGCATACAAAGGCATTAATGCTGGGTAACCACATTGTGTAAAG6810.13691111633826092No Hit
ATCCTAAACTTACCAAATATTTGCCCTTAGATAAAGGCATCAAACCTTAC6660.13389545298279262No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGAATGGCAAACTCCCTCTTTTC6530.13128187807472008No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGATC6220.12504950714008561No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA6170.12404428602159616No Hit
CTATTAATAATATCCTCTTTCAAATGAATGTGAGGAAAAGAGGGAGTTTG6130.12324010912680462No Hit
CAATATGTGGGCCCTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGAT6120.12303906490310675No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA6080.12223488800831518No Hit
CTACTGTACCTGTCTTTAATCCTGAATGGCAAACTCCCTCTTTTCCTCAC5840.11740982663956592No Hit
CTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT5420.10896596924425467No Hit
ATCCTAACCTTACCAAATATTTGCCCTTAGATAAAGGCATCAAACCTTAC5370.10796074812576523No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)