FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488198

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488198
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences410619
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA16990.4137655588270392No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC13400.3263365796516966No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT12990.3163516544533984No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGAAACACCCGTG11530.28079557935701954No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC10610.25839038135108217No Hit
GCACCGGACCATGCAAAACCTGCACAACTCCTGCTCAAGGAACCTCTATG10010.24377829569503603No Hit
CATGTATACAAGCCAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC9740.23720285714981526No Hit
GTCCGGTGCTGGTCGTTGATGATCCTGGAATCAGAGGACAAACGGGCAAC9090.22137309768909866No Hit
TCCTCAGGCCATGCAGTGGAACTCCACCACTTTCCACCAAACTCTTCAAG9070.22088602816723044No Hit
ATATAAAAGAGAGTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATT9070.22088602816723044No Hit
ATATTAACGTTGACATAACCGACTACTAATTCCCTGGATGCTGGATCTTC8760.2133364505782733No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCGGACTTCTTTCCTTCTGTTCG7510.18289460546151054No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTAAAA7460.18167693165684004No Hit
GTTGTGAGTAGGCCTTGAGCCTGAGGGCTCCACCCCAACATTCCCCCATG7100.17290968026321238No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN7070.17217907598041007No Hit
CCTGTAAACAGGCCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCT6600.16073294221650727No Hit
CCCTTATCTCCACCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAA6390.15561871223689114No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGA6380.15537517747595703No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGCACGACAGAAT6330.15415750367128653No Hit
CCTGCATACAGACCACAAAATGCCCCTATCTTATCAACACTTCCGGAAAC6320.15391396891035242No Hit
CGTTAATATGGGCCTAAAATTCAGACAACTATTGTGGTTTCACATTTCCT6280.152939829866616No Hit
GTATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA6160.15001741273540678No Hit
ATTCTGAGCAGGGCTCACTGTTCCTGAACTGGAGCCACCAGCAGGAAAGT6090.1483126694088681No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTGATTCCAGGATCATCAACGACC5800.14125016134177912No Hit
CCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAACTCCACCACTTT5770.1405195570589768No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT5650.1375971399277676No Hit
CCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGGTG5470.13321351423095376No Hit
CTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGCGT5450.13272644470908557No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCGG5440.13248290994815143No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG5380.13102170138254685No Hit
CTCTTTGGAAGGCGGGGATCTTATATAAAAGAGAGTCCACACGTAGCGCC5280.12858635377320582No Hit
ACCTATGGGAGTGGGCCTCAGTCCGTTTCTCTTGGCTCAGTTTACTAGTG5240.12761221472946943No Hit
CATTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATT5120.12468979759826018No Hit
ATCATATTCCTCTGCATCCTGCTGCTATGCCTCATCTTCTTGTTGGTTCT5080.12371565855452378No Hit
TCCAAAGAGTGTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACA5070.12347212379358967No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA5030.12249798474985327No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT5020.12225444998891918No Hit
GTACAGGGTCCCCAGTCTTCGATAAGATTGACGATATGGCTGAGACAGTA4970.12103677618424866No Hit
GTATCGGGAGGCCTTAGAGTCTCCTGAACATTGTACACCTCACCATACGG4950.12054970666238046No Hit
CCTCCTGCATACAGACCACAAAATGCCCCTATCTTATCAACACTTCCGGA4890.11908849809677585No Hit
GCCTGAGGATGAGTGTCCCTTAGAGGTGGAGATAAGGGAGTAGGCTGCCT4790.11665315048743483No Hit
GCTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG4780.11640961572650073No Hit
CAGTTGGACCCTGCATTCAAAGCCAACTCAGAAAATCCAGATTGGGACCT4770.11616608096556662No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCTGCATACAGACCACAAAATGCC4680.1139742681171597No Hit
CTGTAAACAGTATCTGAACCTTTACCCCGTTGCTCGGCAACGGCCTGGTC4670.1137307333562256No Hit
GCCTTAGAGTCTCCTGAACATTGTACACCTCACCATACGGCACTCAGGCA4650.11324366383435738No Hit
CTCTTATGCAAGACCTTGGGCAGGTTCCCGTGGGTGTTCAAGGTGGTCTC4520.11007771194221407No Hit
ATCTGAACCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAGTG4520.11007771194221407No Hit
GCTCAGAATACTGTCTCAGCCATATCGTCAATCTTATCGAAGACTGGGGA4500.10959064242034586No Hit
GTCCAAGGAATACTAACATTGAGGTTCCCGAGATTGAGATCTTCTGCGAC4460.10861650337660946No Hit
GTCCAACTGATGATCGGGGAAGAATCCCAGGGGATTGGGGACAGAAAGAT4440.10812943385474126No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTTTTGGGGGC4410.10739882957193894No Hit
CTCTAAGGCCTCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAA4370.10642469052820254No Hit
GTCGTTGACATTGCTGAAAGTCCAAGAGTTCTCTTATGCAAGACCTTGGG4350.10593762100633433No Hit
CTTCAAAGACTGTGTGTTTAATGAGTGGGAGGAGTTGGGGGAGGAGGTTA4340.10569408624540025No Hit
CCTCTGCACGTCGCATGGAGACCACCTTGAACACCCACGGGAACCTGCCC4320.10520701672353204No Hit
GTACAACAGGCGGAGAAGCGGGCGGTAGAGCCCCAAGCGGCCCCGGGAGG4320.10520701672353204No Hit
ACATAGAGGTTCCTTGAGCAGGAGTTGTGCAGGTTTTGCATGGTCCGGTG4310.10496348196259793No Hit
CTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTGCACTTCGCTTCAC4310.10496348196259793No Hit
CCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAGTGTTTGCTG4280.10423287767979561No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC4230.10301520387512512No Hit
TTGTACACCTCACCATACGGCACTCAGGCAAGCTATTCTGTGTTGGGGTG4210.10252813435325692No Hit
CCTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAAATACCTATGGGAG4210.10252813435325692No Hit
CAATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGG4190.10204106483138872No Hit
GCCCCAACCAGTGGGGGTTGCGTCAGCAAACACTTGGCACAGACCAGGCC4190.10204106483138872No Hit
AGATAAGGGAGTAGGCTGCCTTCCTGACTGCCGATTGGTGGAGGCAGGAG4170.1015539953095205No Hit
CCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAAGAGTTCTCTTATGC4160.1013104605485864No Hit
TCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAACAGAAGGAAA4150.10106692578765229No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG4140.10082339102671821No Hit
GAATACTAACATTGAGGTTCCCGAGATTGAGATCTTCTGCGACGCGGCGA4120.10033632150485No Hit
GACGGGGAGTCCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGTCGGTACAA4110.10009278674391589No Hit
GCGTAAAAGGGGCGGCAAACCCCAAAAGACCCACAATTCGTTGACATACT4110.10009278674391589No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)