FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488199

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488199
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences410619
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA15120.3682245585323621No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT10800.2630175418088301No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGAAACACCCGTG10700.260582194199489No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC10400.253276151371466No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC9240.22502611910311018No Hit
GTCCGGTGCTGGTCGTTGATGATCCTGGAATCAGAGGACAAACGGGCAAC7780.1894700440067313No Hit
CATGTATACAAGCCAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC7590.18484288354898337No Hit
ATATAAAAGAGAGTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATT7540.18362520974431285No Hit
GCACCGGACCATGCAAAACCTGCACAACTCCTGCTCAAGGAACCTCTATG7530.18338167498337876No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN7090.17266614550227827No Hit
TCCTCAGGCCATGCAGTGGAACTCCACCACTTTCCACCAAACTCTTCAAG7040.17144847169760774No Hit
ATATTAACGTTGACATAACCGACTACTAATTCCCTGGATGCTGGATCTTC6960.16950019361013494No Hit
CTCTTTGGAAGGCGGGGATCTTATATAAAAGAGAGTCCACACGTAGCGCC6780.1651165679133211No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTAAAA6330.15415750367128653No Hit
GTTGTGAGTAGGCCTTGAGCCTGAGGGCTCCACCCCAACATTCCCCCATG6240.1519656908228796No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGA5950.14490318275579064No Hit
GTATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA5950.14490318275579064No Hit
CCCTTATCTCCACCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAA5830.14198076562458142No Hit
CCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAACTCCACCACTTT5560.13540532707936068No Hit
ATTCTGAGCAGGGCTCACTGTTCCTGAACTGGAGCCACCAGCAGGAAAGT5300.12907342329507404No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCGGACTTCTTTCCTTCTGTTCG5200.126638075685733No Hit
CCTGTAAACAGGCCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCT5030.12249798474985327No Hit
CTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGCGT5020.12225444998891918No Hit
CTGCCGGACCGTGTGCACTTCGCTTCACCTCTGCACGTCGCATGGAGACC4960.12079324142331455No Hit
CCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGGTG4890.11908849809677585No Hit
ACCTATGGGAGTGGGCCTCAGTCCGTTTCTCTTGGCTCAGTTTACTAGTG4890.11908849809677585No Hit
GCCTTAGAGTCTCCTGAACATTGTACACCTCACCATACGGCACTCAGGCA4780.11640961572650073No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA4750.11567901144369842No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGCACGACAGAAT4710.11470487239996202No Hit
CGTTAATATGGGCCTAAAATTCAGACAACTATTGTGGTTTCACATTTCCT4650.11324366383435738No Hit
CCTGCATACAGACCACAAAATGCCCCTATCTTATCAACACTTCCGGAAAC4530.11032124670314818No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCGG4510.10983417718127997No Hit
ATCATATTCCTCTGCATCCTGCTGCTATGCCTCATCTTCTTGTTGGTTCT4450.10837296861567537No Hit
GTATCGGGAGGCCTTAGAGTCTCCTGAACATTGTACACCTCACCATACGG4430.10788589909380716No Hit
GCCTGAGGATGAGTGTCCCTTAGAGGTGGAGATAAGGGAGTAGGCTGCCT4400.10715529481100484No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCTGCATACAGACCACAAAATGCC4350.10593762100633433No Hit
TCCAAAGAGTGTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACA4290.10447641244072974No Hit
CTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTGCACTTCGCTTCAC4260.10374580815792742No Hit
CCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAGTGTTTGCTG4250.10350227339699332No Hit
CTCTAAGGCCTCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAA4240.10325873863605921No Hit
GCCCCAACCAGTGGGGGTTGCGTCAGCAAACACTTGGCACAGACCAGGCC4150.10106692578765229No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)