FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002488200

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488200
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences128576
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGTCAACAGTATCTGAACCTTTACCCCGTTGCTCGGCAACGGTCTGGTC8360.6501991040318567No Hit
CTCATTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCATTGACA8260.6424216027874564No Hit
GTTTACTGAGAAAGGCCTTGTAAGTTGGCGAGAACGTAAAAGCCTGTTTT6980.5428695868591339No Hit
GAAACGATGTATACTTGCGGGAGAGCACGACAGAATTGTCAGTCCCGATG6010.46742782478845196No Hit
CTCCGTCAACAGTATCTGAACCTTTACCCCGTTGCTCGGCAACGGTCTGG5880.45731707317073167No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTACACCTCACCATACGG5810.4518728222996516No Hit
CTTTTATATAAGATACCCGCCTTCCAAAGAGTGTGTAAATAATGTCTCGT4590.3569873071179691No Hit
TCCAAAGAGTGTGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACA4380.3406545545047287No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCATTGACATTGCTGAGAGTCCAA4060.31576655052264807No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC3970.3087667994026879No Hit
CCTCCCCCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA3860.3002115480338477No Hit
CTGTTTACTGAGAAAGGCCTTGTAAGTTGGCGAGAACGTAAAAGCCTGTT3800.2955450472872076No Hit
CATCCTTTCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGG3770.29321179691388755No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT3740.29087854654056744No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC3680.28621204579392734No Hit
ATTCTGTGTTGGGGTGAGTTAATGAATCTAGCCACCTGGGTGGGAAGTAA3670.2854342956694873No Hit
CAATTCGTTGACATACTTTCCAATCAATAGGCCTGTTTACAGGAAGTTTC3490.27143479342956695No Hit
ACACTCGGGTGTTCCCCCTAGAAAATTGAGAGAAGTCCACCACGAGTCTA3300.25665754106520655No Hit
CATTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCATTGACATT3090.24032478845196614No Hit
GTACCTTCCTGCTGGTGGCTCCAGTTCAGGAACAGTGAGCCCTGCTCAGA2980.23176953708312592No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT2860.2224365355898457No Hit
ACACACGGGTGTTCCCCCTAGAAAATTGAGAGAAGTCCACCACGAGTCTA2820.2193255350920856No Hit
ATATGGAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA2740.21310353409656543No Hit
GGGAATTAGTAGTCAGCTATGTCAACGTTAATATGGGCCTAAAAATCAGA2730.21232578397212543No Hit
GACCTGCTGCGAGCAAAACAAGCGGCTAGGAGTTCCGCAGTATGGATCGG2720.21154803384768542No Hit
GGAACATATTGTACAAAAAATCAAAATGTGTTTTAGGAAACTTCCTGTAA2630.20454828272772524No Hit
AGTCTGTACAACACCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTT2560.1991040318566451No Hit
ATGGAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGA2480.19288203086112493No Hit
GGATAATAAGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACATT2480.19288203086112493No Hit
GCTCAGAATACTGTCTCTGCCATATCGTCAATCTTATCGAAGACTGGGGA2430.18899328023892484No Hit
ATATGGGGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA2370.1843267794922847No Hit
GTTGTGAGTATGCCCTGAGCCTGAGGGCTCCACCCCAACAGTCCCCCATG2350.18277127924340467No Hit
CCTAACCTCCTCCCCCAACTCCTCCCACTCATTAAACACACAGTCTTTGA2190.17032727725236435No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTGTCTCCCAAAAGTAAGA2150.1672162767546043No Hit
CAACGACTTGTGGGTCTTTTGGGGTTTGCCGCTCCTTTTACGCAATGTGG2140.16643852663016426No Hit
CTATTCTGTGTTGGGGTGAGTTAATGAATCTAGCCACCTGGGTGGGAAGT2130.16566077650572425No Hit
CCAAAAGACACCAAATATTCAAGAACAGTTTGTCTCCCAAAAGTAAGACA2130.16566077650572425No Hit
GGAAAGTACAGGGCCCTGACTCTTGGATCTTGAAGAGTTTGGTGGAAAGT2110.1641052762568442No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTAAAA2110.1641052762568442No Hit
CCCGTGTGTCTTGGCCAAAATTCGCAGTCCCAAATCTCCAGTCACTCACC2110.1641052762568442No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAG2090.16254977600796416No Hit
CCCGAGTGTCTTGGCCAAAATTCGCAGTCCCAAATCTCCAGTCACTCACC2090.16254977600796416No Hit
CCCTTACAGTAAATGAAAACAGGAGACTAAAATTAATTATGCCTGCTAGA2030.15788327526132404No Hit
GTCATTGACATTGCTGAGAGTCCAAGAGTCCTCTTATGCAAGACCTTGGG2020.15710552513688403No Hit
GTACAACACCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTTTTGTC2000.15555002488800398No Hit
ATCCCGCGGACGACCCCTCCCGGGGCCGCTTGGGGCTCTACCGCCCGCTT1990.15477227476356398No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC1980.15399452463912394No Hit
GTATAGGGAGGCCTTAGAGTCTCCGGAACATTGTACACCTCACCATACGG1930.15010577401692385No Hit
CGATAAGATTGACGATATGGCAGAGACAGTATTCTGAGCAGGGCTCACTG1930.15010577401692385No Hit
GACCTTTAACCTAATCTCCTCCCCCAACTCCTCCCACTCATTAAACACAC1880.14621702339472376No Hit
ATATTAACGTTGACATAGCTGACTACTAATTCCCTGGATGCTGGATCTTC1840.14310602289696367No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG1840.14310602289696367No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACTCCCAATTACATATCCCATGAA1820.14155052264808363No Hit
ATCTCATCGAAGACTGGGGACCCTGTACCGAACATGGAGAACATCGCATC1790.13921727227476358No Hit
CTCTTTTATATAAGATACCCGCCTTCCAAAGAGTGTGTAAATAATGTCTC1790.13921727227476358No Hit
GTTTACTGAGAAAGGCCTTGTAAGTTGGCGAGAATGTAAAAGCCTGTTTT1780.13843952215032354No Hit
ATCTCAATGTTAGTATTCCTTGGACACATAAGGTGGGAAACTTTACGGGG1780.13843952215032354No Hit
CAAATCTTTCTGTCCCCAATCCCCTGGGATTCTTCCCCGATCATCAGTTG1780.13843952215032354No Hit
CCCTTATCTCCACCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAA1770.13766177202588353No Hit
ACGACTTGTGGGTCTTTTGGGGTTTGCCGCTCCTTTTACGCAATGTGGAT1740.13532852165256348No Hit
CCCCCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGAAAC1730.13455077152812345No Hit
TCCTCAGGCCATGCAGTGGAACTCCAGCACTTTCCACCAAACTCTTCAAG1730.13455077152812345No Hit
GGAAACGATGTATACTTGCGGGAGAGCACGACAGAATTGTCAGTCCCGAT1690.13143977103036336No Hit
TTATACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAACC1670.12988427078148332No Hit
ATATAAGATACCCGCCTTCCAAAGAGTGTGTAAATAATGTCTCGTCTGGA1660.12910652065704328No Hit
CCTTTTACGCAATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTAT1650.12832877053260328No Hit
CGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCTT1640.12755102040816327No Hit
CAATAGGCCTGTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGT1620.12599552015928323No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC1610.1252177700348432No Hit
GCCTTAGAGTCTCCGGAACATTGTACACCTCACCATACGGCACTCAGGCA1560.1213290194126431No Hit
TTATTATCCAGAGCATGTAGTTAATCATTACTTCCAGACGAGACATTATT1540.11977351916376305No Hit
GCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAGGCTTTTACGTT1540.11977351916376305No Hit
GCCTAGCAGCCATGGAAACGATGTATACTTGCGGGAGAGCACGACAGAAT1520.11821801891488302No Hit
GTGTGGACTCTCTTTTATATAAGATACCCGCCTTCCAAAGAGTGTGTAAA1500.11666251866600298No Hit
CATTAAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT1470.11432926829268292No Hit
CCTCAAGGTCGGTCATTGACATTGCTGAGAGTCCAAGAGTCCTCTTATGC1460.1135515181682429No Hit
CTCTTTGGAAGGCGGGTATCTTATATAAAAGAGAGTCCACACGTAGCGCC1450.11277376804380287No Hit
GTCCGGTGCTGGTTGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC1450.11277376804380287No Hit
TCCTTGGACACATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACGG1440.11199601791936287No Hit
ACAATTCGTTGACATACTTTCCAATCAATAGGCCTGTTTACAGGAAGTTT1410.1096627675460428No Hit
GTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGTACAATATGTT1410.1096627675460428No Hit
GTGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACATGCTCTGGAT1400.10888501742160278No Hit
ATATAAAAGAGAGTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATT1400.10888501742160278No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACACCTTGAGTCCCTTTATGCC1390.10810726729716276No Hit
GTACTTTCCTGCTGGTGGCTCCAGTTCAGGAACAGTGAGCCCTGCTCAGA1380.10732951717272274No Hit
GTCCAAGAGTCCTCTTATGCAAGACCTTGGGCAGGTTCCGGTGGGCGTTC1370.10655176704828272No Hit
GTATGGATCGGCAGAGGAGACACAAAGGTTCCACGCATGCGCTGATGGCC1360.10577401692384271No Hit
GCTTTATTCTTCTACGGTACCTTGCTTTAATCCTAAATGGCAAACTCCTT1360.10577401692384271No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG1360.10577401692384271No Hit
GTACAACAGGCGGAGAAGCGGGCGGTAGAGCCCCAAGCGGCCCCGGGAGG1350.10499626679940269No Hit
GCACCGGACCATGCAAAACCTGCACAACTCCTGCTCAAGGAACCTCTATG1330.10344076655052264No Hit
CCGCAGGATTCAGCGCCGACGGGACGTAAACAAAGGACGTCCCGCGCAGG1320.10266301642608262No Hit
GCTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCCGTGG1290.10032976605276256No Hit
GTTTCTCTCCCAAAAGTAAGACAGGAAATGTGAAACCACAATAGTTGTCT1290.10032976605276256No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)