FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002488201

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488201
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences128576
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGTCAACAGTATCTGAACCTTTACCCCGTTGCTCGGCAACGGTCTGGTC6670.5187593330014932No Hit
CTCCGTCAACAGTATCTGAACCTTTACCCCGTTGCTCGGCAACGGTCTGG5990.46587232453957195No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTACACCTCACCATACGG5970.4643168242906919No Hit
GTTTACTGAGAAAGGCCTTGTAAGTTGGCGAGAACGTAAAAGCCTGTTTT5860.45576157292185165No Hit
CTCATTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCATTGACA5090.39587481333997016No Hit
GAAACGATGTATACTTGCGGGAGAGCACGACAGAATTGTCAGTCCCGATG4700.36554255848680933No Hit
CATCCTTTCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGG4120.3204330512692882No Hit
CCTCCCCCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA3860.3002115480338477No Hit
CTTTTATATAAGATACCCGCCTTCCAAAGAGTGTGTAAATAATGTCTCGT3760.2924340467894475No Hit
TCCAAAGAGTGTGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACA3290.2558797909407665No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCATTGACATTGCTGAGAGTCCAA3220.2504355400696864No Hit
CTGTTTACTGAGAAAGGCCTTGTAAGTTGGCGAGAACGTAAAAGCCTGTT2880.22399203583872573No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT2860.2224365355898457No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC2740.21310353409656543No Hit
CAATTCGTTGACATACTTTCCAATCAATAGGCCTGTTTACAGGAAGTTTC2720.21154803384768542No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC2630.20454828272772524No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT2510.19521528123444498No Hit
ATTCTGTGTTGGGGTGAGTTAATGAATCTAGCCACCTGGGTGGGAAGTAA2450.19054878048780488No Hit
ACACTCGGGTGTTCCCCCTAGAAAATTGAGAGAAGTCCACCACGAGTCTA2410.1874377799900448No Hit
GACCTGCTGCGAGCAAAACAAGCGGCTAGGAGTTCCGCAGTATGGATCGG2360.1835490293678447No Hit
ATATGGAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA2300.17888252862120457No Hit
AGTCTGTACAACACCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTT2280.17732702837232453No Hit
GTACCTTCCTGCTGGTGGCTCCAGTTCAGGAACAGTGAGCCCTGCTCAGA2210.1718827775012444No Hit
GTATAGGGAGGCCTTAGAGTCTCCGGAACATTGTACACCTCACCATACGG2190.17032727725236435No Hit
GGGAATTAGTAGTCAGCTATGTCAACGTTAATATGGGCCTAAAAATCAGA2100.16332752613240417No Hit
CTATTCTGTGTTGGGGTGAGTTAATGAATCTAGCCACCTGGGTGGGAAGT2080.16177202588352416No Hit
GCTCAGAATACTGTCTCTGCCATATCGTCAATCTTATCGAAGACTGGGGA2060.1602165256346441No Hit
CAACGACTTGTGGGTCTTTTGGGGTTTGCCGCTCCTTTTACGCAATGTGG2060.1602165256346441No Hit
ATGGAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGA2060.1602165256346441No Hit
GGATAATAAGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACATT2040.15866102538576407No Hit
ACACACGGGTGTTCCCCCTAGAAAATTGAGAGAAGTCCACCACGAGTCTA1950.1516612742658039No Hit
ATATAAGATACCCGCCTTCCAAAGAGTGTGTAAATAATGTCTCGTCTGGA1950.1516612742658039No Hit
ATATGGGGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA1940.15088352414136386No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTGTCTCCCAAAAGTAAGA1930.15010577401692385No Hit
CATTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCATTGACATT1920.14932802389248384No Hit
ATCCCGCGGACGACCCCTCCCGGGGCCGCTTGGGGCTCTACCGCCCGCTT1880.14621702339472376No Hit
GTACAACACCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTTTTGTC1830.14232827277252366No Hit
CTCTTTTATATAAGATACCCGCCTTCCAAAGAGTGTGTAAATAATGTCTC1780.13843952215032354No Hit
CAAATCTTTCTGTCCCCAATCCCCTGGGATTCTTCCCCGATCATCAGTTG1780.13843952215032354No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTAAAA1780.13843952215032354No Hit
ATCTCATCGAAGACTGGGGACCCTGTACCGAACATGGAGAACATCGCATC1720.13377302140368344No Hit
GTTTACTGAGAAAGGCCTTGTAAGTTGGCGAGAATGTAAAAGCCTGTTTT1720.13377302140368344No Hit
GTTGTGAGTATGCCCTGAGCCTGAGGGCTCCACCCCAACAGTCCCCCATG1710.1329952712792434No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC1680.13066202090592335No Hit
ACCTTATTATCCAGAGCATGTAGTTAATCATTACTTCCAGACGAGACATT1670.12988427078148332No Hit
GGAACATATTGTACAAAAAATCAAAATGTGTTTTAGGAAACTTCCTGTAA1650.12832877053260328No Hit
CCCTTACAGTAAATGAAAACAGGAGACTAAAATTAATTATGCCTGCTAGA1600.12444001991040318No Hit
TCCTCAGGCCATGCAGTGGAACTCCAGCACTTTCCACCAAACTCTTCAAG1570.12210676953708312No Hit
GGAAAGTACAGGGCCCTGACTCTTGGATCTTGAAGAGTTTGGTGGAAAGT1560.1213290194126431No Hit
CCCGAGTGTCTTGGCCAAAATTCGCAGTCCCAAATCTCCAGTCACTCACC1560.1213290194126431No Hit
CCCTTATCTCCACCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAA1540.11977351916376305No Hit
GTATGGATCGGCAGAGGAGACACAAAGGTTCCACGCATGCGCTGATGGCC1540.11977351916376305No Hit
ATCTCAATGTTAGTATTCCTTGGACACATAAGGTGGGAAACTTTACGGGG1530.11899576903932303No Hit
TTATACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAACC1520.11821801891488302No Hit
GTGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACATGCTCTGGAT1510.117440268790443No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACTCCCAATTACATATCCCATGAA1510.117440268790443No Hit
ATATTAACGTTGACATAGCTGACTACTAATTCCCTGGATGCTGGATCTTC1480.11510701841712294No Hit
CCTTTTACGCAATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTAT1480.11510701841712294No Hit
CCAAAAGACACCAAATATTCAAGAACAGTTTGTCTCCCAAAAGTAAGACA1470.11432926829268292No Hit
CAATAGGCCTGTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGT1470.11432926829268292No Hit
TTATTATCCAGAGCATGTAGTTAATCATTACTTCCAGACGAGACATTATT1470.11432926829268292No Hit
GTTTCTCTCCCAAAAGTAAGACAGGAAATGTGAAACCACAATAGTTGTCT1470.11432926829268292No Hit
CCCGTGTGTCTTGGCCAAAATTCGCAGTCCCAAATCTCCAGTCACTCACC1460.1135515181682429No Hit
GACCTTTAACCTAATCTCCTCCCCCAACTCCTCCCACTCATTAAACACAC1450.11277376804380287No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAG1450.11277376804380287No Hit
ACGACTTGTGGGTCTTTTGGGGTTTGCCGCTCCTTTTACGCAATGTGGAT1430.11121826779492285No Hit
CATTAAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT1420.11044051767048282No Hit
GCCTTAGAGTCTCCGGAACATTGTACACCTCACCATACGGCACTCAGGCA1370.10655176704828272No Hit
CCTTTATGCCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA1310.1018852663016426No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC1310.1018852663016426No Hit
CCTCTCTTTACGCGGACTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGT1300.10110751617720258No Hit
CCCCCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGAAAC1290.10032976605276256No Hit
GCTTTATTCTTCTACGGTACCTTGCTTTAATCCTAAATGGCAAACTCCTT1290.10032976605276256No Hit
GTACTTTCCTGCTGGTGGCTCCAGTTCAGGAACAGTGAGCCCTGCTCAGA1290.10032976605276256No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)