FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488205

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488205
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences440853
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN20990.47612242629629375No Hit
GTATGTAAATAATGCCTGGTTTTGAAGTAATGATTAACTGCATGTTCAGG12070.2737874075939145No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTGGTTT10360.23499896790993824No Hit
CTCCAAAGGATACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA8820.20006668889629878No Hit
ATCCTAACCTTACCAAATATTTGCCATTGGACAAAGGCATTAAACCATAT8200.18600304409859977No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTGGT7270.16490757690205124No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCGAGG6830.15492692575529712No Hit
GTATATAGGGTTTGCTACTGTCTGTGGTCTCAGGCACCCACTGGAGGTCT6460.14653410547279933No Hit
CATTTAAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT6280.14245111182185446No Hit
GGTTAGGATAGAACCTCGCAGGCATAATTAATTTTAATCTCCTTTTTTCA6230.1413169469188142No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA6090.13814128519030153No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCGCAG5840.13247046067510032No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATCAATAATGTCCTCATGT5780.13110946279145202No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA5720.12974846490780373No Hit
GTCTAGGGTGGACGTTTACAGCTCCTGAAGCCCCAATGGGCGTGTGTTCC5580.12657280317929107No Hit
CCCTATATACACTACGTTTTTCCCTACATGTACCCACCTATGATACAGTT5460.12385080741199447No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT5380.12203614356713008No Hit
TCCACAGAGTATGTAAATAATGCCTGGTTTTGAAGTAATGATTAACTGCA5230.11863364885800935No Hit
CCTTTGGAGTGTGGATTCGCACTCCTACCGCTTACAGACCACCAAATGCC5230.11863364885800935No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC5070.11500432116828058No Hit
CATATAAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT4980.11296282434280815No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC4690.10638466790517473No Hit
TCTACGGAGTTACTCTCTTTTTTGCCTTCTGACTTTTTTCCTTCTATTCG4690.10638466790517473No Hit
ATGTTAATATGGGCCTAAAACTCAGACAAATATTGTGGTTTCACATTTCC4610.10457000406031035No Hit
GTATTAATCAGCTCAGTTTGACCCTCTACCTTCTCGCAAGAACAGAGGAC4570.10366267213787815No Hit
ATATTATCCTGAACATGCAGTTAATCATTACTTCAAAACCAGGCATTATT4410.10003334444814939No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)