FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488208

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488208
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences394138
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN39441.0006647417909462No Hit
CTTTTATATAAGACACCCGCCTTCCAAAGAGTGTGTAAATAATGTCTCGT14290.3625633661306446No Hit
TCCAAAGAGTGTGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTGCA11120.28213468379095646No Hit
GGATAATAAGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACAGT10550.2676727440642618No Hit
CGTTAATATGGGCCTAAAACTCAGACAACTATTGTGGTTTCACATTTCCT8700.22073486951270874No Hit
ATATAAGACACCCGCCTTCCAAAGAGTGTGTAAATAATGTCTCGTCTGGA7850.1991688190430763No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTCACTTCAT7820.19840766432061865No Hit
CCCTTACAGTAAATGAAACCAGGAGACTAAAATTAATTATGCCTGCTAGG7770.19713907311652265No Hit
GTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGTACAATATGTT7380.1872440617245736No Hit
GGGAATTAGTAGTCGGCTATGTCAACGTTAATATGGGCCTAAAACTCAGA7030.17836392329590142No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGGAAGTCCAA7010.17785648681426303No Hit
GGAACATATTGTACAAAAAATCAAAATGTGTTTTAGGAAACTTCCTGTAA6560.16643916597739877No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGAAAGA6320.16034992819773786No Hit
ATATGTTCCTGTGGCAATGTACCCCAACTCCCAATTACATATCCCATGAA6240.1583201822711842No Hit
CAGTAAATGAAACCAGGAGACTAAAATTAATTATGCCTGCTAGGTTTTAT6230.158066464030365No Hit
AGTCTGTACAACACCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTT6010.15248466273234249No Hit
GTTTTATCCCACTGTTACTAAATATTTGCCCTTAGATAAAGGGATCAAAC5900.1496937620833312No Hit
GGGACTTAGTAGTCGGCTATGTCAACGTTAATATGGGCCTAAAACTCAGA5780.14664914319350075No Hit
GTTCAAATACTGTTGACGTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAA5370.13624669531991332No Hit
CAATAGGTCTGTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGT5340.1354855405974557No Hit
TTGACATACTTTCCAATCAATAGGTCTGTTTACAGGAAGTTTCCTAAAAC5320.1349781041158173No Hit
GATGTAAGCAATTTGTGGGGCCCCTTACAGTAAATGAAACCAGGAGACTA5260.13345579467090207No Hit
ACTTACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT5240.13294835818926365No Hit
CTCTTTTATATAAGACACCCGCCTTCCAAAGAGTGTGTAAATAATGTCTC5220.13244092170762525No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC5180.13142604874434843No Hit
GTGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTGCATGCTCTGGAT4950.1255905292055067No Hit
TTATTATCCAGAGCATGCAGTTAATCATTACTTCCAGACGAGACATTATT4920.12482937448304908No Hit
ACTACATGCTCTGGATAATAAGGTTTGATCCCTTTATCTAAGGGCAAATA4880.12381450151977226No Hit
CCTTTATGCCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA4770.12102360087076101No Hit
AAATGAAACCAGGAGACTAAAATTAATTATGCCTGCTAGGTTTTATCCCA4760.12076988262994179No Hit
ATATAAAAGAGAGTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATT4630.11747154549929212No Hit
GTACAACACCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTTTTGTC4540.11518808133191927No Hit
ACTGCATGCTCTGGATAATAAGGTTTGATCCCTTTATCTAAGGGCAAATA4520.11468064485028087No Hit
GTTACTAAATATTTGCCCTTAGATAAAGGGATCAAACCTTATTATCCAGA4470.11341205364618484No Hit
GTTGATAGATGTAAGCAATTTGTGGGGCCCCTTACAGTAAATGAAACCAG4430.11239718068290802No Hit
ATCTAAGGGCAAATATTTAGTAACAGTGGGATAAAACCTAGCAGGCATAA4330.10985999827471597No Hit
CCTCCTGCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA4330.10985999827471597No Hit
ATGTAATTGGGAGTTGGGGTACATTGCCACAGGAACATATTGTACAAAAA4280.10859140707061993No Hit
CTTTTACGCAATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATA4260.10808397058898152No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAGTTTTCTAGGGGGAACACCCGAG4250.10783025234816232No Hit
GTTGTAAGTATGCCCTGAGCCTGAGGGCTCCACCCCAACAGTCCCCCATG4250.10783025234816232No Hit
GAACAGTTTCTCTCCCAAAAGAAAGACAGGAAATGTGAAACCACAATAGT4100.10402447873587423No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC4100.10402447873587423No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)