FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488209

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488209
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences394138
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN39140.9930531945663701No Hit
CTTTTATATAAGACACCCGCCTTCCAAAGAGTGTGTAAATAATGTCTCGT13880.3521609182570572No Hit
TCCAAAGAGTGTGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTGCA10990.2788363466603068No Hit
GGATAATAAGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACAGT10850.2752842912888379No Hit
CCCTTACAGTAAATGAAACCAGGAGACTAAAATTAATTATGCCTGCTAGG8100.20551177506355642No Hit
ATATAAGACACCCGCCTTCCAAAGAGTGTGTAAATAATGTCTCGTCTGGA8070.20475062034109878No Hit
CGTTAATATGGGCCTAAAACTCAGACAACTATTGTGGTTTCACATTTCCT8060.2044969021002796No Hit
GTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGTACAATATGTT7950.20170600145126832No Hit
AGTCTGTACAACACCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTT7230.18343828811228555No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTCACTTCAT7070.17937879625917827No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGGAAGTCCAA6660.16897634838559084No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGAAAGA6450.16364826532838753No Hit
GGGAATTAGTAGTCGGCTATGTCAACGTTAATATGGGCCTAAAACTCAGA6330.16060364643855704No Hit
ATATGTTCCTGTGGCAATGTACCCCAACTCCCAATTACATATCCCATGAA6260.1588276187528226No Hit
GGGACTTAGTAGTCGGCTATGTCAACGTTAATATGGGCCTAAAACTCAGA6230.158066464030365No Hit
CTCTTTTATATAAGACACCCGCCTTCCAAAGAGTGTGTAAATAATGTCTC6030.15299209921398088No Hit
GTTTTATCCCACTGTTACTAAATATTTGCCCTTAGATAAAGGGATCAAAC5890.149440043842512No Hit
GGAACATATTGTACAAAAAATCAAAATGTGTTTTAGGAAACTTCCTGTAA5660.1436045243036703No Hit
GTGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTGCATGCTCTGGAT5530.14030618717302062No Hit
GTTCAAATACTGTTGACGTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAA5370.13624669531991332No Hit
GTACAACACCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTTTTGTC5190.13167976698516762No Hit
CAGTAAATGAAACCAGGAGACTAAAATTAATTATGCCTGCTAGGTTTTAT5060.12838142985451795No Hit
ACTTACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT4970.1260979656871451No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAGTTTTCTAGGGGGAACACCCGAG4970.1260979656871451No Hit
GATGTAAGCAATTTGTGGGGCCCCTTACAGTAAATGAAACCAGGAGACTA4940.12533681096468752No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC4930.12508309272386828No Hit
TTGACATACTTTCCAATCAATAGGTCTGTTTACAGGAAGTTTCCTAAAAC4890.12406821976059146No Hit
CCTTTATGCCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA4890.12406821976059146No Hit
GTTGATAGATGTAAGCAATTTGTGGGGCCCCTTACAGTAAATGAAACCAG4880.12381450151977226No Hit
ATGTAATTGGGAGTTGGGGTACATTGCCACAGGAACATATTGTACAAAAA4700.11924757318502656No Hit
GTTGTAAGTATGCCCTGAGCCTGAGGGCTCCACCCCAACAGTCCCCCATG4660.11823270022174974No Hit
TTATTATCCAGAGCATGCAGTTAATCATTACTTCCAGACGAGACATTATT4560.11569551781355769No Hit
ACTACATGCTCTGGATAATAAGGTTTGATCCCTTTATCTAAGGGCAAATA4550.1154417995727385No Hit
ATCTAAGGGCAAATATTTAGTAACAGTGGGATAAAACCTAGCAGGCATAA4370.11087487123799279No Hit
GTTACTAAATATTTGCCCTTAGATAAAGGGATCAAACCTTATTATCCAGA4350.11036743475635437No Hit
GGATAAAACCTAGCAGGCATAATTAATTTTAGTCTCCTGGTTTCATTTAC4290.10884512531143915No Hit
CTTTATCTAAGGGCAAATATTTAGTAACAGTGGGATAAAACCTAGCAGGC4280.10859140707061993No Hit
CAATAGGTCTGTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGT4250.10783025234816232No Hit
AAATGAAACCAGGAGACTAAAATTAATTATGCCTGCTAGGTTTTATCCCA4230.1073228158665239No Hit
CTTTTACGCAATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATA4190.10630794290324708No Hit
CCTCCTGCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA4180.10605422466242788No Hit
CTTCAAAGACTGTGTGTTTACTGAGTGGGAGGAGTTGGGGGAGGAGATTA4090.10377076049505503No Hit
GCTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG3980.10097985984604378No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)