FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488210

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488210
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences406455
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCAAAGAGTGTGCAAATAATGTCTGGTCTGGAAGTAATGATTAACTACA17230.4239091658363165No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN15930.3919253053843599No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT13640.3355845050497595No Hit
CTTTTATATAAGATACCCGCCTTCCAAAGAGTGTGCAAATAATGTCTGGT13600.33460038626662236No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC12760.31393389182074277No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC10510.2585772102692795No Hit
CTCATTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA9980.2455376363927126No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA9600.2361885079529099No Hit
GTTGTGAGTATGCCCTGAGCCTGAGGGCTCCACCCCAACAGTCCCCCATG8220.20223640993467912No Hit
ATGTATACTTGCGGGAGAGCACGACAGAATTGTCAGTCCCAATGAGTTTT7980.19633169723585636No Hit
CCCTTACAGTAAATGAAAACAGGAGACTAAAATTAATTATGCCTGCTAGG7730.19018095484124933No Hit
GGATAATAAGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACATT7500.18452227183821088No Hit
TTATTATCCAGAGCATGTAGTTAATCATTACTTCCAGACCAGACATTATT7290.17935564822674097No Hit
TCCTCAGGCCATGCAGTGGAACTCCACCACTTTCCACCAAACTCTTCAAG7160.17615726218154532No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG7070.17394299491948678No Hit
CATGTATACAAGCAAAACAGGCTTTCACTTTCTCGCCAACTTACAAGGCC7000.17222078704899682No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACACCCAATTACATATCCCATGAA6970.17148269796164398No Hit
CGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCCT6840.16828431191644833No Hit
CTCTTTGGAAGGCGGGTATCTTATATAAAAGAGAGTCCACACGTAGCGCC6760.16631607435017406No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGA6660.16385577739233126No Hit
GTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGTACAATATGTT6420.1579510646935085No Hit
ATATAAAAGAGAGTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATT6320.1554907677356657No Hit
ATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA6160.1515542926031172No Hit
CCCAATTACATATCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTT5700.14023692659704026No Hit
AGTATACATCGTTCCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTG5680.1397448672054717No Hit
ATATAAGATACCCGCCTTCCAAAGAGTGTGCAAATAATGTCTGGTCTGGA5600.13777662963919746No Hit
GGAACATATTGTACAAAAAATCAAAATGTGTTTTAGGAAACTTCCTGTAA5590.13753059994341316No Hit
GCCTAGCAGCCATGGGAACGATGTATACTTGCGGGAGAGCACGACAGAAT5580.13728457024762888No Hit
CAATTACATATCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTTTG5580.13728457024762888No Hit
CCTCCTGCATATAGACCACAAAATGCCCCTATCTTATCAACACTTCCGGA5550.13654648116027604No Hit
CCCTTATCTCTACCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAA5420.1333480951150804No Hit
GTGGATATCCTGCCTTAATGCCTTTATATGCATGTATACAAGCAAAACAG5240.12891956059096335No Hit
GCCTTCCTGACTGCCGATTGGTGGAGGCAGGAGGAGGAGCTGCTGGCACA5210.12818147150361048No Hit
ATTCTGAGCAGGGCTCACTGTTCCTGAACTGGAGCCACCAGCAGGAAAGT5180.12744338241625763No Hit
TAGATAAAGGGATCAAACCTTATTATCCAGAGCATGTAGTTAATCATTAC5170.12719735272047336No Hit
CATTTGAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT5140.12645926363312052No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCGCCTCACCATACGG5100.1254751448499834No Hit
ATTACATATCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTTTGTG4930.12129264002165062No Hit
ATATTAACGTTGACATAGCTGACTACTAATTCCCTGGATGCTGGATCTTC4910.12080058063008205No Hit
CCTGTAAACAGACCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCT4860.11957043215116066No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC4860.11957043215116066No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG4850.11932440245537637No Hit
ATACCACATCATCCATATAACTGAAAGCCAGACAGTGGGGGAAAGCCCTA4850.11932440245537637No Hit
ATGTGGATATCCTGCCTTAATGCCTTTATATGCATGTATACAAGCAAAAC4790.11784822428067068No Hit
CAATAGGTCTGTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGT4700.11563395701861215No Hit
CATTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATT4680.11514189762704358No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTATGCC4610.1134196897565536No Hit
TATCCACATTGCGTGAAAGGGGCGGCAAACCCCAAAAGACCCACAATTCG4570.1124355709734165No Hit
TGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGAA4530.11145145219027935No Hit
GCTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG4530.11145145219027935No Hit
GTATACATGCATATAAAGGCATTAAGGCAGGATATCCACATTGCGTGAAA4520.11120542249449508No Hit
TCAGCAAGGCAGCCTACTCCCTTATCTCTACCTCTAAGGGACACTCATCC4500.11071336310292652No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT4500.11071336310292652No Hit
CCCATAGGTATTTTACGAAAGCCCAAGATGATGGGATGGGAATACAGGTG4450.1094832146240051No Hit
TCCTTGGACACATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACGG4420.10874512553665228No Hit
GCTCAGAATACTGTCTCTGCCATATCGTCAATCTTATCGAAGACTGGGGA4400.10825306614508372No Hit
CCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAAGAGTCCTCTTATGC4370.10751497705773086No Hit
GACCAGACCGTTGCCGAGCAACGGGGTAAAGGTTCAGATATTGTTTACTT4340.10677688797037803No Hit
CTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGA4320.10628482857880947No Hit
GTTGATAGATGTAAGCAATTTGTGGGGCCCCTTACAGTAAATGAAAACAG4310.10603879888302517No Hit
CTCTTTTATATAAGATACCCGCCTTCCAAAGAGTGTGCAAATAATGTCTG4270.10505468009988805No Hit
GGGTAAAGGTTCAGATATTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGA4230.10407056131675092No Hit
GCACGGGACCATGCAGAGGACTAAGCAAAACCTGCACAACTACTGCTCAA4190.1030864425336138No Hit
TCTATCAACAATGTCCTCCTGCAAATGAATGTCAGGAAAAGAAGGAGTTT4190.1030864425336138No Hit
CGTGTGGACTCTCTTTTATATAAGATACCCGCCTTCCAAAGAGTGTGCAA4190.1030864425336138No Hit
TCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAATAGAAGGAAA4140.1018562940546924No Hit
ATGTAATTGGGTGTTGGGGCACATTGCCACAGGAACATATTGTACAAAAA4090.100626145575771No Hit
ACTACATGCTCTGGATAATAAGGTTTGATCCCTTTATCTAAGGGCAAATA4090.100626145575771No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)