FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488211

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488211
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences406455
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN15810.3889729490349485No Hit
TCCAAAGAGTGTGCAAATAATGTCTGGTCTGGAAGTAATGATTAACTACA14060.34591775227269933No Hit
CTTTTATATAAGATACCCGCCTTCCAAAGAGTGTGCAAATAATGTCTGGT12030.2959737240284902No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC11090.2728469326247678No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC8930.21970451833536309No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT8150.20051420206418916No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA7870.19362537058222928No Hit
CCCTTACAGTAAATGAAAACAGGAGACTAAAATTAATTATGCCTGCTAGG7330.18033976700987808No Hit
GGATAATAAGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACATT6890.16951446039536971No Hit
GTTGTGAGTATGCCCTGAGCCTGAGGGCTCCACCCCAACAGTCCCCCATG6530.1606573913471356No Hit
TTATTATCCAGAGCATGTAGTTAATCATTACTTCCAGACCAGACATTATT6170.15180032229890147No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG6090.14983208473262724No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGA5960.14663369868743156No Hit
ATGTATACTTGCGGGAGAGCACGACAGAATTGTCAGTCCCAATGAGTTTT5950.1463876689916473No Hit
CTCATTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA5800.14269722355488307No Hit
TCCTCAGGCCATGCAGTGGAACTCCACCACTTTCCACCAAACTCTTCAAG5760.14171310477174595No Hit
CCCTTATCTCTACCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAA5720.14072898598860883No Hit
ATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA5670.13949883750968742No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACACCCAATTACATATCCCATGAA5590.13753059994341316No Hit
CTCTTTGGAAGGCGGGTATCTTATATAAAAGAGAGTCCACACGTAGCGCC5450.13408618420243323No Hit
GTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGTACAATATGTT5230.12867353089517905No Hit
ATATTAACGTTGACATAGCTGACTACTAATTCCCTGGATGCTGGATCTTC5090.12522911515419913No Hit
AGTATACATCGTTCCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTG4900.12055455093429776No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC4820.11858631336802351No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCGCCTCACCATACGG4730.116372046105965No Hit
CATGTATACAAGCAAAACAGGCTTTCACTTTCTCGCCAACTTACAAGGCC4680.11514189762704358No Hit
GCCTAGCAGCCATGGGAACGATGTATACTTGCGGGAGAGCACGACAGAAT4680.11514189762704358No Hit
GTGGATATCCTGCCTTAATGCCTTTATATGCATGTATACAAGCAAAACAG4670.1148958679312593No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTT4560.11218954127763221No Hit
ATATAAAAGAGAGTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATT4510.11095939279871081No Hit
GACCAGACCGTTGCCGAGCAACGGGGTAAAGGTTCAGATATTGTTTACTT4510.11095939279871081No Hit
CGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCCT4480.11022130371135797No Hit
ATATAAGATACCCGCCTTCCAAAGAGTGTGCAAATAATGTCTGGTCTGGA4440.10923718492822083No Hit
CTCTTTTATATAAGATACCCGCCTTCCAAAGAGTGTGCAAATAATGTCTG4300.1057927691872409No Hit
GGAACATATTGTACAAAAAATCAAAATGTGTTTTAGGAAACTTCCTGTAA4300.1057927691872409No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA4300.1057927691872409No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTATGCC4260.10480865040410378No Hit
ATGTGGATATCCTGCCTTAATGCCTTTATATGCATGTATACAAGCAAAAC4260.10480865040410378No Hit
ATGTAATTGGGTGTTGGGGCACATTGCCACAGGAACATATTGTACAAAAA4150.10210232375047668No Hit
CCTCCTGCATATAGACCACAAAATGCCCCTATCTTATCAACACTTCCGGA4150.10210232375047668No Hit
CCCAATTACATATCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTT4140.1018562940546924No Hit
GTATACATGCATATAAAGGCATTAAGGCAGGATATCCACATTGCGTGAAA4070.10013408618420243No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)