FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488212

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488212
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences499301
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN66641.334665862876301No Hit
ATTAATAGGTGTCAGCAATTTGTAGGCCCTCTCACTGTAAATGAAAAGAG14320.28680094772491943No Hit
CTTATATAGAATACCAGCCTTCCACAGAGTATGTAAATAATGACGGGTCT14300.2864003877420634No Hit
GTATGTAAATAATGACGGGTCTGGAAGTAATGATTAACTACCTGATCAGG12610.2525530691907286No Hit
GTCTTTGAAGTAAGCCTCAAGGTCGGTCGTTGACATTGCTGGGAGTCCAA11320.2267169502965145No Hit
GGATAATAAGGTTTAATTCCTTTGTCTAAGGGCAAATATTTTGTACTGTT11140.22311191045081025No Hit
GTTCATATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAA10500.2102939909994172No Hit
CATTTAAATACTAACAAAACAAAAAGATGGGGTTATTCCCTAAACTTCAT10430.20889203105942106No Hit
GTGTAAAGGGAGCGGCAAAGCCCAAAAGACCCACAATTCTTTGACACACT10390.208090911093709No Hit
TTTAAATACTAACAAAACAAAAAGATGGGGTTATTCCCTAAACTTCATGG9970.19967915145373233No Hit
CCCTTTACACAATGTGGATACCCTGCATTAATGCCTTTGTATGCATGTAT9840.19707551156516812No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC9650.1932701917280358No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGATCACCCGTG8960.17945087231950266No Hit
ATATGGTCCTGTGGTAATGATCCCCAACTTCCAATTATGTATCCCATGAA8700.17424359254237426No Hit
GTACAAAATATTTGCCCTTAGACAAAGGAATTAAACCTTATTATCCTGAT8480.1698374327309579No Hit
GTATATGAACCTTTACCCCGTTGCCCGGCAACGGCCTGGTCTGTGCCAAG7860.15742007326242086No Hit
GTGGTAATGATCCCCAACTTCCAATTATGTATCCCATGAAGTTTAGGGAA7530.15081083354529634No Hit
CTCTTATATAGAATACCAGCCTTCCACAGAGTATGTAAATAATGACGGGT7450.14920859361387218No Hit
GGACCATATTGTACAAAAAATTAGACACTGTTTTAGAAAACTTCCTGTTA7150.1432001938710317No Hit
GGATATTATTAATAGGTGTCAGCAATTTGTAGGCCCTCTCACTGTAAATG7140.1429999138796037No Hit
ATGTAAGACCTTGGGCAGGATCTGATGGGCGTTCACGGTGGTCGCCATGC7110.14239907390531964No Hit
TCCACAGAGTATGTAAATAATGACGGGTCTGGAAGTAATGATTAACTACC7100.14219879391389162No Hit
TTATTATCCTGATCAGGTAGTTAATCATTACTTCCAGACCCGTCATTATT7090.1419985139224636No Hit
GTACGGGACCCTGCAAAACCTGCACGACTCCTGCTCAAGGCAACTCTATG6970.1395951540253274No Hit
GGGTAAAGGTTCATATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGA6930.13879403405961532No Hit
TTCTAAAACAGTGTCTAATTTTTTGTACAATATGGTCCTGTGGTAATGAT6720.134588154239627No Hit
GTCTAAGGGCAAATATTTTGTACTGTTAGGATAGAATCTAGCGGGCATAA6600.1321847943424908No Hit
ATTTTGTACTGTTAGGATAGAATCTAGCGGGCATAATTAATTTCAATCTT6550.1311833943853507No Hit
ATCTAGGAGATCTCGAACGGAAGGAAAGAAGTCAGAAGGCAAAAACGAGA6550.1311833943853507No Hit
GTTTTAGAAAACTTCCTGTTAATAGGCCTATTGATTGGAAAGTGTGTCAA6530.1307828344024947No Hit
ATCCTAACAGTACAAAATATTTGCCCTTAGACAAAGGAATTAAACCTTAT6490.12998171443678264No Hit
GTATACATGCATACAAAGGCATTAATGCAGGGTATCCACATTGTGTAAAG6410.1283794745053585No Hit
GTTAGGATAGAATCTAGCGGGCATAATTAATTTCAATCTTCTCTTTTCAT6350.1271777945567904No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT6310.12637667459107835No Hit
GTGGATACCCTGCATTAATGCCTTTGTATGCATGTATACAAGCTAAACAG6230.1247744346596542No Hit
CATTACCACAGGACCATATTGTACAAAAAATTAGACACTGTTTTAGAAAA6180.1237730347025141No Hit
GTCCCGTACTGGTTGTTGTGGATCCTGGAATTAGAGGACAAACGGGCAAC6170.1235727547110861No Hit
CTATATAAGAGGGAAACCACACGTAGCGCCTCATTTTTCGGGTCACCATA5950.11916659489966974No Hit
CATGTATACAAGCTAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC5870.1175643549682456No Hit
CTCCGAAAGAGACCAAATACTCAAGTACGGTTTCTCGTCCAAAAGTAAGG5850.11716379498538958No Hit
ATTCTATCCTAACAGTACAAAATATTTGCCCTTAGACAAAGGAATTAAAC5790.11596211503682148No Hit
CCTATTAATAATATCCTCATGTAAATGTATCTTAGGAAAAGAAGGAGTTT5780.11576183504539346No Hit
ATAATATCCTCATGTAAATGTATCTTAGGAAAAGAAGGAGTTTGCCATTC5720.11456015509682536No Hit
GGTTCATATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTA5610.11235707519111718No Hit
GAGTATGTAAATAATGACGGGTCTGGAAGTAATGATTAACTACCTGATCA5610.11235707519111718No Hit
CCCTTAGACAAAGGAATTAAACCTTATTATCCTGATCAGGTAGTTAATCA5580.11175623521683313No Hit
CTGTAAATGAAAAGAGAAGATTGAAATTAATTATGCCCGCTAGATTCTAT5390.10795091537970083No Hit
ATTATTAATAGGTGTCAGCAATTTGTAGGCCCTCTCACTGTAAATGAAAA5310.10634867544827668No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC5200.10414559554256851No Hit
ATTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA5180.10374503555971247No Hit
CATTTACATGAGGATATTATTAATAGGTGTCAGCAATTTGTAGGCCCTCT5110.10234307561971635No Hit
GTCTTACATAAGAGGACTCTTGGACTCCCAGCAATGTCAACGACCGACCT5060.10134167566257628No Hit
CTTTCCAATCAATAGGCCTATTAACAGGAAGTTTTCTAAAACAGTGTCTA5060.10134167566257628No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)