FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488213

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488213
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences499301
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN66551.3328633429534489No Hit
GTATGTAAATAATGACGGGTCTGGAAGTAATGATTAACTACCTGATCAGG13980.2799914280163669No Hit
ATTAATAGGTGTCAGCAATTTGTAGGCCCTCTCACTGTAAATGAAAAGAG13670.27378274828209836No Hit
CTTATATAGAATACCAGCCTTCCACAGAGTATGTAAATAATGACGGGTCT12660.2535544691478687No Hit
GGATAATAAGGTTTAATTCCTTTGTCTAAGGGCAAATATTTTGTACTGTT10550.21129539095655725No Hit
CCCTTTACACAATGTGGATACCCTGCATTAATGCCTTTGTATGCATGTAT10510.21049427099084522No Hit
GTGTAAAGGGAGCGGCAAAGCCCAAAAGACCCACAATTCTTTGACACACT9850.1972757915565961No Hit
GTTCATATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAA9670.19367075171089185No Hit
GTCTTTGAAGTAAGCCTCAAGGTCGGTCGTTGACATTGCTGGGAGTCCAA9660.1934704717194638No Hit
CATTTAAATACTAACAAAACAAAAAGATGGGGTTATTCCCTAAACTTCAT9100.1822547921994949No Hit
TTTAAATACTAACAAAACAAAAAGATGGGGTTATTCCCTAAACTTCATGG9050.18125339224235482No Hit
ATATGGTCCTGTGGTAATGATCCCCAACTTCCAATTATGTATCCCATGAA9010.18045227227664273No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGATCACCCGTG8990.18005171229378672No Hit
GTATATGAACCTTTACCCCGTTGCCCGGCAACGGCCTGGTCTGTGCCAAG8780.17584583247379837No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC8750.17524499249951433No Hit
GTACAAAATATTTGCCCTTAGACAAAGGAATTAAACCTTATTATCCTGAT8380.1678346328166777No Hit
CTCTTATATAGAATACCAGCCTTCCACAGAGTATGTAAATAATGACGGGT7680.15381503341671657No Hit
GTGGTAATGATCCCCAACTTCCAATTATGTATCCCATGAAGTTTAGGGAA7380.1478066336738761No Hit
GGATATTATTAATAGGTGTCAGCAATTTGTAGGCCCTCTCACTGTAAATG7110.14239907390531964No Hit
TTCTAAAACAGTGTCTAATTTTTTGTACAATATGGTCCTGTGGTAATGAT7090.1419985139224636No Hit
TTATTATCCTGATCAGGTAGTTAATCATTACTTCCAGACCCGTCATTATT7040.14099711396532352No Hit
GTCTAAGGGCAAATATTTTGTACTGTTAGGATAGAATCTAGCGGGCATAA6830.13679123414533517No Hit
GTATACATGCATACAAAGGCATTAATGCAGGGTATCCACATTGTGTAAAG6690.13398731426534297No Hit
GTACGGGACCCTGCAAAACCTGCACGACTCCTGCTCAAGGCAACTCTATG6570.13158395436820675No Hit
GGACCATATTGTACAAAAAATTAGACACTGTTTTAGAAAACTTCCTGTTA6510.13038227441963868No Hit
ATTTTGTACTGTTAGGATAGAATCTAGCGGGCATAATTAATTTCAATCTT6300.1261763945996503No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCCGTTCG6300.1261763945996503No Hit
ATCCTAACAGTACAAAATATTTGCCCTTAGACAAAGGAATTAAACCTTAT6270.12557555462536626No Hit
TCCACAGAGTATGTAAATAATGACGGGTCTGGAAGTAATGATTAACTACC6240.12497471465108222No Hit
GGGTAAAGGTTCATATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGA6210.12437387467679817No Hit
GTTTTAGAAAACTTCCTGTTAATAGGCCTATTGATTGGAAAGTGTGTCAA6160.12337247471965807No Hit
CATTACCACAGGACCATATTGTACAAAAAATTAGACACTGTTTTAGAAAA6090.12197051477966198No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT6080.12177023478823394No Hit
CCTATTAATAATATCCTCATGTAAATGTATCTTAGGAAAAGAAGGAGTTT6010.12036827484823782No Hit
GTTAGGATAGAATCTAGCGGGCATAATTAATTTCAATCTTCTCTTTTCAT5970.11956715488252577No Hit
GGTTCATATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTA5920.1185657549253857No Hit
CTCCGAAAGAGACCAAATACTCAAGTACGGTTTCTCGTCCAAAAGTAAGG5920.1185657549253857No Hit
CATGTATACAAGCTAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC5610.11235707519111718No Hit
CCCTTAGACAAAGGAATTAAACCTTATTATCCTGATCAGGTAGTTAATCA5570.11155595522540512No Hit
CTTTCCAATCAATAGGCCTATTAACAGGAAGTTTTCTAAAACAGTGTCTA5470.10955315531112496No Hit
GTGGATACCCTGCATTAATGCCTTTGTATGCATGTATACAAGCTAAACAG5430.10875203534541289No Hit
GTGTCTAATTTTTTGTACAATATGGTCCTGTGGTAATGATCCCCAACTTC5390.10795091537970083No Hit
CTATATAAGAGGGAAACCACACGTAGCGCCTCATTTTTCGGGTCACCATA5360.10735007540541676No Hit
GTCTTACATAAGAGGACTCTTGGACTCCCAGCAATGTCAACGACCGACCT5310.10634867544827668No Hit
ATTATTAATAGGTGTCAGCAATTTGTAGGCCCTCTCACTGTAAATGAAAA5290.10594811546542067No Hit
GAGTATGTAAATAATGACGGGTCTGGAAGTAATGATTAACTACCTGATCA5190.1039453155511405No Hit
ATGTAAGACCTTGGGCAGGATCTGATGGGCGTTCACGGTGGTCGCCATGC5110.10234307561971635No Hit
GTCTGGAAGTAATGATTAACTACCTGATCAGGATAATAAGGTTTAATTCC5060.10134167566257628No Hit
CTTCAAAGACTGTGTGTTTAAAGACTGGGAGGAGTTGGGGGAGGAGATTA5000.10013999571400818No Hit
GTCCCGTACTGGTTGTTGTGGATCCTGGAATTAGAGGACAAACGGGCAAC5000.10013999571400818No Hit
CATTTACATGAGGATATTATTAATAGGTGTCAGCAATTTGTAGGCCCTCT5000.10013999571400818No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)