FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002488215

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488215
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences312999
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC13470.4303528126287944No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA12600.40255719666836No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTGATTATGCCTGCCAGG12220.39041658280058406No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG11990.3830683165121933No Hit
AGCGAGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATACTCTAGAA10720.34249310700673163No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGACC10700.3418541273294803No Hit
GTATGTAAATAATGTCTAGTTTGGAAGTAATGATTAACTGTATGTTCAGG10680.34121514765222893No Hit
ATCCTAACCTTACCAAATATTTGCCCTTAGATAAAGGCATTAAACCTTAT10020.3201288183029339No Hit
GGTTAGGATAGAACCTGGCAGGCATAATCAATTTTAATCTCCTTTTTTCA9600.3067102450806552No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCATTGACATTGCTGAGAGTCCAA9350.29872299911501315No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTGTTGATAATATCCTCCTTC7890.2520774826756635No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG7100.2268377854242346No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN7090.22651829558560888No Hit
ATTGACAACAGTGCCAGCAGCGCCTCCTCCTGCCTCCACCAATCGGCAGT6930.221406458167598No Hit
GTGTAAAAGGGGCGGCAAAGCCCAGAAGACCCACAATTCTTTGACATACT6910.2207674784903466No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTTTTTCCGTCTATTCG6550.20926584429982203No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTCTA6460.20639043575219088No Hit
CTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGA6300.20127859833418No Hit
GCTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG6170.1971252304320461No Hit
GCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT6080.19424982188441495No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTAAGGCA5980.19105492349815814No Hit
CTTTTACACAATGTGGTTACCCAGCATTAATGCCTTTATATGCATGTATA5930.18945747430502974No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTAGATAAAGGCATTAAAC5670.181150738500762No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG5610.1792337994690079No Hit
CTCCAAAAGACACCAAATACTCTAGAACAGTTTCTCTTCCAAAAGTAAGA5500.17571941124412538No Hit
GGATAGAACCTGGCAGGCATAATCAATTTTAATCTCCTTTTTTCATTAAC5480.17508043156687403No Hit
CTCTAGAACAGTTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA5470.17476094172824833No Hit
GCACTAAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCAC5460.17444145188962265No Hit
GAGTATGTAAATAATGTCTAGTTTGGAAGTAATGATTAACTGTATGTTCA5450.17412196205099695No Hit
GTATACATGCATATAAAGGCATTAATGCTGGGTAACCACATTGTGTAAAA5330.1702880839874888No Hit
CTCCATATTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGG5190.16581522624672923No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC5180.16549573640810353No Hit
TCCACAGAGTATGTAAATAATGTCTAGTTTGGAAGTAATGATTAACTGTA5120.16357879737634945No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTTTACC5110.16325930753772375No Hit
AGTCTGTACAACATCTTGAATCCCTTTTTACCTCTATTACCAATTTTCTT5080.16230083802184672No Hit
GGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATATTTGGTAAGGTT4930.15750849044246149No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACTGCCGTG4870.15559155141070738No Hit
CCTCTCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA4810.1536746123789533No Hit
GTAGTAAGCTATGTCAATGTTAACATGGGCCTAAAAATCAGACAACTATT4770.15239665302445055No Hit
TCCTAGAAGTAGAGGACAAACGGGCAACATACCTTGGTAGTCCAGAAGAA4540.14504838673605985No Hit
ATATGTTCCTGTGGCAAGGTACCCCAACTTCCAATTACATATCCCATGAA4410.140895018833926No Hit
ATTATCAACAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAA4400.14057552899530032No Hit
GTTTGGGACTCTACCGTCCCCTTCTTCGTCTGCCGTTCCGACCGACCACG4400.14057552899530032No Hit
GGATATTATCAACAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATG4270.13642216109316643No Hit
CTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTCAC4250.13578318141591506No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA4240.13546369157728938No Hit
ATACAGCACTAAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTG4180.1335467525455353No Hit
CTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGTCTAGTTT4100.13099083383652982No Hit
GGTCAGGTCTGTGCCAAGTGTTTGCTGACGCAACCCCCACTGGTTGGGGC4000.127795935450273No Hit
CCTCAAGGTCGGTCATTGACATTGCTGAGAGTCCAAGAGTCCTCTTATGT3990.1274764456116473No Hit
GTGTTGGGGTGAGTTGATGAATCTGGCCACCTGGGTGGGAAGTAATTTGG3980.12715695577302163No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC3940.1258789964185189No Hit
TCCTTTGTCTACGTCCCTTCCGCGCTGAATCCCGCGGACGACCCCTCTCG3930.12555950657989323No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC3910.12492052690264185No Hit
CATTTAAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAATTTCAT3870.12364256754813913No Hit
GATCCATACTGCGGAACTCCTAGCAGCTTGTTTTGCTCGCAGCCGGTCTG3860.12332307770951344No Hit
TCACCATACAGCACTAAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGA3860.12332307770951344No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA3800.12140613867775935No Hit
ACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTT3800.12140613867775935No Hit
GTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATATTTGGTA3790.12108664883913368No Hit
GGAACATATTGTACAAAAATTGAAACAATGTTTTCGGAAACTTCCTATAA3760.12012817932325662No Hit
GTCTACGTCCCTTCCGCGCTGAATCCCGCGGACGACCCCTCTCGGGGCCG3700.11821124029150253No Hit
GTGCTGGAGAAGGAAAAGGGATGAATAGGCAGAATGCACAGGATTTTTAG3670.11725277077562549No Hit
CTCACCAACCTCTTGTCCTCCAATTTGTCCTGGTTATCGCTGGATGTGTC3650.11661379109837412No Hit
GTCATTGACATTGCTGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGG3600.1150163419052457No Hit
ATGTTAACATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC3600.1150163419052457No Hit
GACCTGACCGTTGCCGAGCAACGGGGTAAAGGTTCAGATATTGTTTACAC3590.11469685206662002No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCTGATGGCC3580.11437736222799433No Hit
GTCCAAGGGATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGAC3510.11214093335761455No Hit
CTCTAAGGCCTCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCCCGAA3410.10894603497135773No Hit
ATTTACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACA3400.10862654513273204No Hit
GGTTCTATCCTAACCTTACCAAATATTTGCCCTTAGATAAAGGCATTAAA3390.10830705529410638No Hit
ATGTAAGACCTTGGGCAAGACCTGGCGGGCGTTCACGGTGGTTTCCATGC3390.10830705529410638No Hit
GTTTCCGAAAACATTGTTTCAATTTTTGTACAATATGTTCCTGTGGCAAG3380.10798756545548069No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTC3330.10639011626235229No Hit
TCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCCCGAATAGACGGAAA3320.1060706264237266No Hit
AGCGGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATACTCTAGAA3310.10575113658510091No Hit
GCCTTCCACAGAGTATGTAAATAATGTCTAGTTTGGAAGTAATGATTAAC3290.10511215690784954No Hit
CCCTTAATTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTGCCACAG3260.1041536873919725No Hit
CTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTCACATTCATTTGAAGG3180.10159776868296704No Hit
AATATGGAGAGCACCACATCAGGATTCCTAGGACCCCTGCTCGTGTTACA3180.10159776868296704No Hit
GATTAACTGTATGTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGGGC3130.10000031948983862No Hit
CTTCCACAGAGTATGTAAATAATGTCTAGTTTGGAAGTAATGATTAACTG3130.10000031948983862No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)