FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488218

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488218
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences539740
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTAGTTTGGAAGTAATGATTGACTATGTGTTCAGG33740.6251157964946085No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN24910.4615185089116982No Hit
GGATATTATCAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAACG24280.44984622225515986No Hit
ATCCTAACCGTACCAAATATTTGCCCTTAGATAAAGGCATTAAACCTTAT21120.3912995145810946No Hit
CATTTAAACCCTACTAAAACTAAACGTTGGGGCTACTCACTTAACTTCAT17190.31848667877126025No Hit
CTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGATTATGCCTGCTAGG17060.3160781116834031No Hit
GTAGTAAGCTATGTCAATGTTAATATGGGCCTAAAAATCAGACAACTATT16510.30588802015785377No Hit
CTACTGTACCTGTCTTTAATCCTGATTGGCAAACTCCCTCTTTTCCTCAC15560.28828695297735946No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA14960.277170489494942No Hit
GTGTAAAGGGAGCGGCAAAGCCCAGAAGCCCCACAATTCGTTGACATACT14100.2612368918368103No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAAATC13980.25901359914032684No Hit
ACAATATGTAGGCCCTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGA13940.25827250157483234No Hit
GGTTAGGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTTTTTTCG13540.25086152591988736No Hit
CTTATATAAAATACCCGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT13050.2417830807425798No Hit
CCCTTTACACAATGTGGTTACCCAGCATTAATGCCTTTGTATGCATGTAT12390.22955497091192056No Hit
GGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATATTTGGTACGGTT12300.22788750138955793No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGATTGGCAAACTCCCTCTTTTC11620.21528884277615148No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTATCTCTTCCAAAAGTAAGA11460.2123244525141735No Hit
ATGTAGGCCCTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGATTATG11190.20732204394708562No Hit
CTCAAGAACAGTATCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA10950.20287545855411865No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC10440.1934264645940638No Hit
TATCAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAACGAAAAAA10050.18620076333049246No Hit
GTTCTATCCTAACCGTACCAAATATTTGCCCTTAGATAAAGGCATTAAAC10010.18545966576499795No Hit
GCTTACTACTGCTTCCCTGGATGCTGGGTCTTCCAAATTACTTCCCACCC9980.18490384259087708No Hit
AGTCTGTACAACATCTTGAATCCCTTTTTACCGCTGTTACCAATTTTCTT9670.17916033645829474No Hit
ATGTAAGACCTTGGGCAAGGCCTGGCGGGCGTTCACGGTGGTCTCCATGC9580.1774928669359321No Hit
GAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTGACTATGTGTTCA9530.176566494979064No Hit
ATATGTTCCTGTGGTAAGGTACCCCAACTTCCAATTACATATCCCATGAA9520.17638122058769037No Hit
GGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTTTTTTCGTTAAC9480.17564012302219587No Hit
GTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATATTTGGTA9450.175084299848075No Hit
CTCTTATATAAAATACCCGCCTTCCACAGAGTATGTAAATAATGCCTAGT9120.1689702449327454No Hit
CAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAACGAAAAAAGGA9110.16878497054137176No Hit
GTATACATGCATACAAAGGCATTAATGCTGGGTAACCACATTGTGTAAAG9030.16730277541038277No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC9010.16693222662763554No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG8920.1652647571052729No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTGACTATG8910.16507948271389927No Hit
CTGTAAGAGGGCCTACATATTGTTGACATCTATTGATAATATCCTCTTTC8870.1643383851484048No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC8830.16359728758291028No Hit
GCACTAAGACAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTAGCCAC8370.15507466557972358No Hit
ATTATCAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAACGAAAA8270.15322192166598733No Hit
ATATAAAATACCCGCCTTCCACAGAGTATGTAAATAATGCCTAGTTTGGA7490.13877051913884464No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTTTACC7460.13821469596472377No Hit
ATTTACATACTCTGTGGAAGGCGGGTATTTTATATAAGAGAGAAACTACT7440.13784414718197652No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG7440.13784414718197652No Hit
ATCTAAGGGCAAATATTTGGTACGGTTAGGATAGAACCTAGCAGGCATAA7420.13747359839922926No Hit
ACATATTGTTGACATCTATTGATAATATCCTCTTTCAAATGAATGTGAGG7390.13691777522510837No Hit
CAATGTTAGTATCCCTTGGACTCACAAGGTGGGAAACTTTACTGGGCTTT7350.1361766776596139No Hit
CTTCAAAGACTGTGTATTTAAAGACTGGGAGGAGTTGGGGGAGGAGATTA7340.13599140326824027No Hit
GTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCCTG7310.13543558009411938No Hit
CTTTAATCCTGATTGGCAAACTCCCTCTTTTCCTCACATTCATTTGAAAG7310.13543558009411938No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA7310.13543558009411938No Hit
CCAAAAGACACCAAATACTCAAGAACAGTATCTCTTCCAAAAGTAAGACA7070.1309889947011524No Hit
TCCTCAGGCCGTGCAGTGGAACTCCAACACATTCCACCAAGCTCTGATAG7050.13061844591840516No Hit
ATTTACAGGAAGTTTCCGAAAACATTGTTTGATTTTGTGTACAATATGTT6690.12394856782895468No Hit
GCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT6640.12302219587208656No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA6600.12228109830659208No Hit
CTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAAATCATT6590.12209582391521845No Hit
ATATGTAGGCCCTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGATTA6570.1217252751324712No Hit
GGCCTACATATTGTTGACATCTATTGATAATATCCTCTTTCAAATGAATG6540.12116945195835031No Hit
GTATAAAGAATTTGGAGCTTCTGCGGAGTTACTCTCTTTTTTGCCTTCTG6490.12024308000148219No Hit
GTTAGTATCCCTTGGACTCACAAGGTGGGAAACTTTACTGGGCTTTATTC6450.11950198243598768No Hit
GTTTGGAAGTAATGATTGACTATGTGTTCAGGATAATAAGGTTTAATGCC6450.11950198243598768No Hit
TTATTATCCTGAACACATAGTCAATCATTACTTCCAAACTAGGCATTATT6190.11468484826027348No Hit
GCCTGAGGATGACTGTCTCTTAGAGGTGGAGAGATGGGAGTAGGCTGTCT6180.11449957386889985No Hit
GCTTTATTCTTCTACTGTACCTGTCTTTAATCCTGATTGGCAAACTCCCT6080.11264682995516359No Hit
CAATAGGTCTATTTACAGGAAGTTTCCGAAAACATTGTTTGATTTTGTGT6070.11246155556378996No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT6050.11209100678104274No Hit
ATGATTGACTATGTGTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGG6040.11190573238966911No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG6030.11172045799829548No Hit
CACTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTACCACAG6020.11153518360692186No Hit
TTTACATACTCTGTGGAAGGCGGGTATTTTATATAAGAGAGAAACTACTC5970.11060881165005373No Hit
ATTTATGCCTACAGCCTCCCAGTACAAAAATCATTAATCTAATCTCCTCC5860.10857079334494386No Hit
TATTAACATTGACATAGCTTACTACTGCTTCCCTGGATGCTGGGTCTTCC5720.10597695186571313No Hit
CAATATGTTCCTGTGGTAAGGTACCCCAACTTCCAATTACATATCCCATG5660.10486530551747139No Hit
ATTGACAACAGTACCAGCAGCGCCTCCTCCTGCCTCCACCATTCGGCAGT5620.10412420795197688No Hit
TCACCATACAGCACTAAGACAAGCTATTCTGTGTTGGGGTGAGTTGATGA5620.10412420795197688No Hit
GTACAACATCTTGAATCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC5600.10375365916922963No Hit
GTGGTAAGGTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGTGAG5600.10375365916922963No Hit
AATATGGAGAGCACCACATCAGGATTCCTAGGACCCCTGCTCGTGTTACA5490.10171564086411977No Hit
ATGTAAATAATGCCTAGTTTGGAAGTAATGATTGACTATGTGTTCAGGAT5480.10153036647274614No Hit
CAACAATATGTAGGCCCTCTTACAGTTAACGAAAAAAGGAGATTAAAATT5480.10153036647274614No Hit
CTGTAAGGGGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATACTC5470.10134509208137252No Hit
CTATTGATAATATCCTCTTTCAAATGAATGTGAGGAAAAGAGGGAGTTTG5460.10115981768999889No Hit
GGTCTATTTACAGGAAGTTTCCGAAAACATTGTTTGATTTTGTGTACAAT5450.10097454329862526No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)