FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488219

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488219
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences539740
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTAGTTTGGAAGTAATGATTGACTATGTGTTCAGG38980.7221995775743877No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN24720.4579982954755994No Hit
GGATATTATCAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAACG20200.37425427057472116No Hit
ATCCTAACCGTACCAAATATTTGCCCTTAGATAAAGGCATTAAACCTTAT18560.34386927038944676No Hit
CTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGATTATGCCTGCTAGG16580.30718494089746917No Hit
GTAGTAAGCTATGTCAATGTTAATATGGGCCTAAAAATCAGACAACTATT16150.2992181420684033No Hit
CATTTAAACCCTACTAAAACTAAACGTTGGGGCTACTCACTTAACTTCAT15630.28958387371697486No Hit
CTACTGTACCTGTCTTTAATCCTGATTGGCAAACTCCCTCTTTTCCTCAC15000.2779115870604365No Hit
GGTTAGGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTTTTTTCG14690.27216808092785416No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAAATC13880.25716085522659055No Hit
GTGTAAAGGGAGCGGCAAAGCCCAGAAGCCCCACAATTCGTTGACATACT13090.24252417830807424No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA12890.23881869048060178No Hit
CCCTTTACACAATGTGGTTACCCAGCATTAATGCCTTTGTATGCATGTAT12770.23659539778411826No Hit
CTTATATAAAATACCCGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT12460.2308518916515359No Hit
GGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATATTTGGTACGGTT12460.2308518916515359No Hit
ACAATATGTAGGCCCTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGA12120.22455256234483267No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTATCTCTTCCAAAAGTAAGA12010.22251454403972282No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGATTGGCAAACTCCCTCTTTTC11640.21565939155889874No Hit
ATGTAGGCCCTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGATTATG10780.19972579390076703No Hit
CTCAAGAACAGTATCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA10760.19935524511801977No Hit
GGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTTTTTTCGTTAAC10710.19842887316115165No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG10670.19768777559565717No Hit
GTTCTATCCTAACCGTACCAAATATTTGCCCTTAGATAAAGGCATTAAAC10610.1965761292474154No Hit
GAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTGACTATGTGTTCA10520.1949086597250528No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC10240.18972097676659133No Hit
CTCTTATATAAAATACCCGCCTTCCACAGAGTATGTAAATAATGCCTAGT9850.18249527550301997No Hit
GCTTACTACTGCTTCCCTGGATGCTGGGTCTTCCAAATTACTTCCCACCC9830.18212472672027272No Hit
GTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATATTTGGTA9810.18175417793752546No Hit
ATATGTTCCTGTGGTAAGGTACCCCAACTTCCAATTACATATCCCATGAA9760.18082780598065734No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC9540.17675176937043763No Hit
CTGTAAGAGGGCCTACATATTGTTGACATCTATTGATAATATCCTCTTTC9030.16730277541038277No Hit
TATCAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAACGAAAAAA8940.16563530588802017No Hit
GTATACATGCATACAAAGGCATTAATGCTGGGTAACCACATTGTGTAAAG8830.16359728758291028No Hit
AGTCTGTACAACATCTTGAATCCCTTTTTACCGCTGTTACCAATTTTCTT8760.1623003668432949No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTGACTATG8590.15915070218994332No Hit
CTTCAAAGACTGTGTATTTAAAGACTGGGAGGAGTTGGGGGAGGAGATTA8530.15803905584170155No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA8420.1560010375365917No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC8030.14877533627302034No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA7720.143031830140438No Hit
CAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAACGAAAAAAGGA7650.14173490940082262No Hit
GTTTGGAAGTAATGATTGACTATGTGTTCAGGATAATAAGGTTTAATGCC7620.14117908622670175No Hit
ATATAAAATACCCGCCTTCCACAGAGTATGTAAATAATGCCTAGTTTGGA7460.13821469596472377No Hit
GCACTAAGACAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTAGCCAC7380.13673250083373478No Hit
ACATATTGTTGACATCTATTGATAATATCCTCTTTCAAATGAATGTGAGG7380.13673250083373478No Hit
ATTATCAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAACGAAAA7370.13654722644236114No Hit
TCCTCAGGCCGTGCAGTGGAACTCCAACACATTCCACCAAGCTCTGATAG7350.1361766776596139No Hit
ATCTAAGGGCAAATATTTGGTACGGTTAGGATAGAACCTAGCAGGCATAA7210.13358283618038316No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTTTACC7110.1317300922666469No Hit
GTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCCTG7020.1300626227442843No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG6950.1287657020046689No Hit
CTTTAATCCTGATTGGCAAACTCCCTCTTTTCCTCACATTCATTTGAAAG6930.12839515322192166No Hit
GTATAAAGAATTTGGAGCTTCTGCGGAGTTACTCTCTTTTTTGCCTTCTG6880.12746878126505354No Hit
ATTTACATACTCTGTGGAAGGCGGGTATTTTATATAAGAGAGAAACTACT6690.12394856782895468No Hit
GGCCTACATATTGTTGACATCTATTGATAATATCCTCTTTCAAATGAATG6590.12209582391521845No Hit
ATGTAAGACCTTGGGCAAGGCCTGGCGGGCGTTCACGGTGGTCTCCATGC6520.12079890317560307No Hit
ATTTACAGGAAGTTTCCGAAAACATTGTTTGATTTTGTGTACAATATGTT6510.12061362878422945No Hit
ATTTATGCCTACAGCCTCCCAGTACAAAAATCATTAATCTAATCTCCTCC6460.11968725682736131No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG6400.11857561047911958No Hit
ATGATTGACTATGTGTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGG6390.11839033608774595No Hit
TTATTATCCTGAACACATAGTCAATCATTACTTCCAAACTAGGCATTATT6320.11709341534813057No Hit
GCCTGAGGATGACTGTCTCTTAGAGGTGGAGAGATGGGAGTAGGCTGTCT6310.11690814095675695No Hit
CAATGTTAGTATCCCTTGGACTCACAAGGTGGGAAACTTTACTGGGCTTT6220.11524067143439433No Hit
GTTAGTATCCCTTGGACTCACAAGGTGGGAAACTTTACTGGGCTTTATTC6140.11375847630340534No Hit
CTATTGATAATATCCTCTTTCAAATGAATGTGAGGAAAAGAGGGAGTTTG6130.11357320191203173No Hit
ATATGTAGGCCCTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGATTA6110.11320265312928447No Hit
ATGTAAATAATGCCTAGTTTGGAAGTAATGATTGACTATGTGTTCAGGAT6090.11283210434653722No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT6080.11264682995516359No Hit
GGTCTATTTACAGGAAGTTTCCGAAAACATTGTTTGATTTTGTGTACAAT5990.11097936043280097No Hit
GTGGTAAGGTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGTGAG5990.11097936043280097No Hit
GCTTTATTCTTCTACTGTACCTGTCTTTAATCCTGATTGGCAAACTCCCT5980.11079408604142735No Hit
GTACAATATGTTCCTGTGGTAAGGTACCCCAACTTCCAATTACATATCCC5940.11005298847593285No Hit
CCAAAAGACACCAAATACTCAAGAACAGTATCTCTTCCAAAAGTAAGACA5870.10875606773631749No Hit
GTGTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATATTTGG5790.10727387260532849No Hit
GCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT5780.10708859821395486No Hit
GTACAACATCTTGAATCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC5770.10690332382258123No Hit
GCCTTTATCTAAGGGCAAATATTTGGTACGGTTAGGATAGAACCTAGCAG5740.10634750064846038No Hit
CTCCATATTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGG5670.10505057990884499No Hit
CAATAGGTCTATTTACAGGAAGTTTCCGAAAACATTGTTTGATTTTGTGT5630.1043094823433505No Hit
CAATATGTTCCTGTGGTAAGGTACCCCAACTTCCAATTACATATCCCATG5600.10375365916922963No Hit
CACTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTACCACAG5580.10338311038648239No Hit
ATAATATCCTCTTTCAAATGAATGTGAGGAAAAGAGGGAGTTTGCCAATC5530.10245673842961425No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAA5530.10245673842961425No Hit
CTGTAAGGGGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATACTC5520.10227146403824063No Hit
GGAACATATTGTACACAAAATCAAACAATGTTTTCGGAAACTTCCTGTAA5510.10208618964686703No Hit
GTTTAATGCCTTTATCTAAGGGCAAATATTTGGTACGGTTAGGATAGAAC5430.10060399451587802No Hit
CTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAAATCATT5420.1004187201245044No Hit
CTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGAAGAGATACTGTTCT5420.1004187201245044No Hit
TTTACATACTCTGTGGAAGGCGGGTATTTTATATAAGAGAGAAACTACTC5410.10023344573313077No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)