FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488221

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488221
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences408659
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN22570.5522942110659499No Hit
CTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGATCATGCCTGCTAGG18670.45686012054059744No Hit
ATCCTAACCTTACCAAATATTTGCCCTTAGATAAAGGCATCAAACCTTAT18360.44927433385781296No Hit
GTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATATGTTCAGG17800.43557097726955724No Hit
GGATATTATCAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAACG17410.42602756821702203No Hit
ATTTACAGGAAGTTTCCTAAAACATTGTTTGATTTTGTGTACAATATGTT16730.40938777807414006No Hit
ATCTTGAATCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTCTTTGGGT13560.33181699167276385No Hit
GTGTAAAGGGAGCGGCAAAACCCAAAAGACCCACAATTCTTTGACATACT12580.3078361176433163No Hit
GGATAATAAGGTTTGATGCCTTTATCTAAGGGCAAATATTTGGTAAGGTT11850.2899728135193401No Hit
GGTTAGGATAGAACCTAGCAGGCATGATCAATTTTAATCTCCTTTTTTCG11170.27333302337645815No Hit
CTACTGTACCTGTCTTTAATCCTGAATGGCAAACTCCCTCTTTTCCTCAC10810.2645237227125794No Hit
GTTCAGGATAATAAGGTTTGATGCCTTTATCTAAGGGCAAATATTTGGTA10100.24714982418104092No Hit
GTATGTAAATAATGCCTAGCTTGGAAGTAATGATTAACTATATGTTCAGG9910.24250047105288272No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTAGATAAAGGCATCAAAC9750.23858522631338108No Hit
CATTTAAACCCTCATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT9150.22390305854024994No Hit
ACTATATGTTCAGGATAATAAGGTTTGATGCCTTTATCTAAGGGCAAATA9120.22316895015159335No Hit
CTATATGTTCAGGATAATAAGGTTTGATGCCTTTATCTAAGGGCAAATAT8890.21754078583855976No Hit
CTCCAAAAGACACCAAATACTCAAGAACAACTTCTCTTCCAAAAGTAAGA8660.21191262152552617No Hit
CCCTTTACACAATGTGGATACCCAGCATTAATGCCTTTGTATGCATGTAT8560.20946559356333763No Hit
GTTTTGAAGTAATGATTAACTATATGTTCAGGATAATAAGGTTTGATGCC8430.20628445721249258No Hit
GTATACATGCATACAAAGGCATTAATGCTGGGTATCCACATTGTGTAAAG8240.20163510408433438No Hit
CCTGTAAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCT8180.20016688730702129No Hit
GTCTTTAATCCTGAATGGCAAACTCCCTCTTTTCCTCACATTCATTTAAA7920.19380461460533108No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTGCCACAG7820.1913575866431426No Hit
GTTTGGAAGTAATGATTAACTATATGTTCAGGATAATAAGGTTTGATGCC7730.1891552614771729No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA7360.18010125801707536No Hit
TATCAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAACGAAAAAA7170.17545190488891718No Hit
GTCTATAATCCTGAATGGCAAACTCCCTCTTTTCCTCACATTCATTTAAA7160.17520720209269833No Hit
CCTATAAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCT7000.17129195735319666No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGAATGGCAAACTCCCTCTTTTC6990.1710472545569778No Hit
CTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT6890.16860022659478932No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC6700.16395087346663112No Hit
CATTTAAAAGAGGATATTATCAATAGATGTCAACAATATGTGGGCCCTCT6630.16223795389309914No Hit
GTAGTAAGCTATGTCAATGTTAATATGGGCCTAAAACTCAGACAAATATT6370.15587568119140896No Hit
ATCTAAGGGCAAATATTTGGTAAGGTTAGGATAGAACCTAGCAGGCATGA6350.15538627559897128No Hit
ATAATAAGGTTTGATGCCTTTATCTAAGGGCAAATATTTGGTAAGGTTAG6330.15489687000653357No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTGATAATATCCTCTTTT6310.15440746441409586No Hit
ATATGTTCCTGTGGCAAGGTACCCCAACTTCCAATTACATATCCCATGAA6300.154162761617877No Hit
GGATAGAACCTAGCAGGCATGATCAATTTTAATCTCCTTTTTTCGTTAAC6150.15049221967459422No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC6050.1480451917124057No Hit
ATTATCAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAACGAAAA5870.14364054138046634No Hit
CAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAACGAAAAAAGGA5810.14217232460315324No Hit
CAATATGTGGGCCCTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGAT5730.14021470223340243No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGTCTTGTAAGTTGGCGA5710.1397252966409647No Hit
ATTTATAGGAAGTTTCCTAAAACATTGTTTGATTTTGTGTACAATATGTT5690.13923589104852702No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG5540.13556534910524423No Hit
CTTTAATCCTGAATGGCAAACTCCCTCTTTTCCTCACATTCATTTAAAAG5410.13238421275439916No Hit
ATGTTAATATGGGCCTAAAACTCAGACAAATATTGTGGTTTCACATTTCC5260.12871367081111637No Hit
CTCTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT5210.1274901568300221No Hit
CCAAAAGACACCAAATACTCAAGAACAACTTCTCTTCCAAAAGTAAGACA5060.1238196148867393No Hit
CCTTTATCTAAGGGCAAATATTTGGTAAGGTTAGGATAGAACCTAGCAGG4990.12210669531320735No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATA4980.1218619925169885No Hit
GATTAAAATTGATCATGCCTGCTAGGTTCTATCCTAACCTTACCAAATAT4960.1213725869245508No Hit
GAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATATGTTCA4940.12088318133211308No Hit
GGAACATATTGTACACAAAATCAAACAATGTTTTAGGAAACTTCCTGTAA4900.11990437014723768No Hit
GCTTGGAAGTAATGATTAACTATATGTTCAGGATAATAAGGTTTGATGCC4800.11745734218504915No Hit
GTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTC4720.11549971981529833No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATGAGGGTTTAAATGTATA4550.11133977227957784No Hit
CTTACAGTTAACGAAAAAAGGAGATTAAAATTGATCATGCCTGCTAGGTT4540.11109506948335898No Hit
CTATTGATAATATCCTCTTTTAAATGAATGTGAGGAAAAGAGGGAGTTTG4540.11109506948335898No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC4530.11085036668714013No Hit
GTTTCTCTCTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATG4490.10987155550226473No Hit
ATCTGGAATCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTCTTTGGGT4480.10962685270604587No Hit
GCTTTATTCTTCTACTGTACCTGTCTTTAATCCTGAATGGCAAACTCCCT4320.10571160796654423No Hit
GATTAACTATATGTTCAGGATAATAAGGTTTGATGCCTTTATCTAAGGGC4250.10399868839301225No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG4240.10375398559679341No Hit
ATTTACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACT4190.10253047161569914No Hit
CACATATTGTTGACATCTATTGATAATATCCTCTTTTAAATGAATGTGAG4150.10155166043082375No Hit
GTCCCCTTCTTCGTCTGCCGTTCCAGCCGACCACGGGGCGCACCTCTCTT4140.10130695763460489No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)