FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002488222

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488222
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences210344
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATATAGAATGCCTGCTTTCCATAAAGTATGCAAATAGTGTCTGGTCT13400.6370516867607348No Hit
CTCTTATATAGAATGCCTGCTTTCCATAAAGTATGCAAATAGTGTCTGGT13400.6370516867607348No Hit
GTGTATATGTGCCACATTTTCTTAATCCAATCTATCATTGTTGGACATTT8310.3950671281329632No Hit
CCTCCTGCTTATAGACCACCAAATGCCCCTATCTTATCCACACTTCCGGA8130.3865097174152816No Hit
ATTCCTCTACTGTTCCTACTTTTAATCCTGACTGGTTAACTCCTTCTTTT7820.3717719545126079No Hit
CCTTTATACCGCTGTTACCAATTTTTTGTTATCTGTGGGTATCCATTTAA7540.35846042672954775No Hit
ACAAGAAACACACCGCCTGTAACACGAGCAGGGGTCCTAGGAGTCCTGAT6750.3209029019130567No Hit
CAAGAAACACACCGCCTGTAACACGAGCAGGGGTCCTAGGAGTCCTGATG6320.3004601985319287No Hit
GGATAATAGGGTTTAATTCCCTTTTCCATAGGAAAATATTTGGTAACCTT6190.29427984634693644No Hit
GGCATACAGAGGCATGAGAGCAGGATAACCACATTGAGTAAAGGGGGCGG5690.27050926102004336No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAATACAAAGATC5660.26908302590042976No Hit
ATATAAGAGAGAATCCACACGTAGCGCCTCATTTTGTGGGTCACCATATT5610.26670596736774044No Hit
GTATTCCATGGTGTATATGTGCCACATTTTCTTAATCCAATCTATCATTG5500.261476438595824No Hit
GTATATACCCAAAGGACTATAAATCAGGCTGATATAAAGACACATACACA5140.24436161716046095No Hit
GTCTTTGATGTATTCTTCGATCCAGGTGATTGACCTTCCTGAAAGTCCAA5010.23818126497546876No Hit
CTATTCCTCTACTGTTCCTACTTTTAATCCTGACTGGTTAACTCCTTCTT4390.20870573917012133No Hit
CTATATAAGAGAGAATCCACACGTAGCGCCTCATTTTGTGGGTCACCATA4210.2001483284524398No Hit
GGGTAAAGGTTCATGTATTGTTGACATAGAAAGGCCTTGTAAGTTGGCGA4200.19967291674590193No Hit
CTATTATCCTGATAACGTGGTTAATCATTATTTTAAGACCAGACACTATT4120.19586962309359907No Hit
CTTTTACACTGTTGGTGGGACTGTAAACTAGTTCAACCATTGTGGAAGTC4080.1939679762674476No Hit
GTTTCCGTCCGAGGGTTTGGAACAGCAGCAAGAGGGAAACATAGAGGTTC3980.18921385920206898No Hit
GTCCCGTGCTGGTGGTCGTGGATCCTGGAAGTAGAGGACAAACGGGCAAC3970.18873844749553115No Hit
ATGCAAATAGTGTCTGGTCTTAAAATAATGATTAACCACGTTATCAGGAT3930.1868368006693797No Hit
GAGTAAAGGGGGCGGCAAAGCCCAAAAGACCCACAATGCGTTGACAAACT3840.18255809531053893No Hit
GTATAAAGGGACTCAAGATGCTGCACAGATTTGGCCCCCAATACCAGATC3800.18065644848438747No Hit
CTTTTAATCCTGACTGGTTAACTCCTTCTTTTCCTGACATTCATTTACAT3740.1778039782451603No Hit
TGTCTGTGCCGATGAGTTTCGCTCCAGACCGGCTGCGAGCGAAACAAGCT3730.17732856653862245No Hit
GTGCTGGTGAGGATGTGGAGAAATGGGAACACTTTTACACTGTTGGTGGG3460.16449245046210018No Hit
TCCTTGGACTCATAAGGTGGGAAATTTTACGGGACTCTATTCCTCTACTG3430.16306621534248658No Hit
CCATAAAGTATGCAAATAGTGTCTGGTCTTAAAATAATGATTAACCACGT3430.16306621534248658No Hit
GTATGCAAATAGTGTCTGGTCTTAAAATAATGATTAACCACGTTATCAGG3430.16306621534248658No Hit
AGTCTAGACTCCGTGGTATTGTGAGGATTTTTGTCAACAAGAAACACACC3400.161639980222873No Hit
ATGTAAGACTGTTGGCAAGCTTCGAGGGGCGTTCACGGTGGTCTCCATGC3360.15973833339672155No Hit
GCACGGGACCATGCAAAACCTGCACAACTCTTGCTCAAGGAACCTCTATG3310.15736127486403226No Hit
ATCTGGTATTGGGGGCCAAATCTGTGCAGCATCTTGAGTCCCTTTATACC3290.15641045145095653No Hit
GGTCCTAGGAGTCCTGATGTGATGTTGTCCATGTTCGTAGCAGGGCCCCC3160.15023009926596434No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGGG3140.14927927585288858No Hit
TTCCCCCACTGTCTGGCTTTTAGTTATATGGATGATCTGGTATTGGGGGC3060.14547598220058572No Hit
GCTATATTGTGCTGGGGTGAGTTAATGACTTTGGCTTCCTGGGTGGGCAA3060.14547598220058572No Hit
ACTCTATTCCTCTACTGTTCCTACTTTTAATCCTGACTGGTTAACTCCTT3050.14500057049404785No Hit
TCCCATTACTGGGTATATACCCAAAGGACTATAAATCAGGCTGATATAAA3040.14452515878751No Hit
CTTCCCAATCAATAGGGCGATTTACAGGAAGTTTACGAAAACAATCTTTG3010.1430989236678964No Hit
GATTAAAATTAGTTATGCCATCCAGATTTTTTCCTAAGGTTACCAAATAT3010.1430989236678964No Hit
CAACAACAACAAGGACAATTGGCCAATGGCAAACAAGGTAGGAGTGGGAG3010.1430989236678964No Hit
GTTCCTCGTTGACAAAAATCCTCACAATACCACGGAGTCTAGACTCGTGG2930.1392956300155935No Hit
GGCACAGGGTGTGCTTACAACATTGCCAGCAGATCCGCCTCCTGCTTCCA2910.13834480660251777No Hit
CTGTATGCCTGTATAACTGCTAAACAGGCTTTTGTCTTTTCGCCAACTTA2890.13739398318944204No Hit
ATGTGATCTTGTGGTAATGATCCCCAACTGCCAATAACATAACCCATGAA2870.13644315977636634No Hit
GTTAGTATTCCTTGGACTCATAAGGTGGGAAATTTTACGGGACTCTATTC2790.13263986612406345No Hit
GCACCGACAACTCTGTTGTCCTCTCTAGGAAGTACACCTCCTTCCCATGG2790.13263986612406345No Hit
CCTTTACTCAATGTGGTTATCCTGCTCTCATGCCTCTGTATGCCTGTATA2740.13026280759137412No Hit
TAGTAAACCTGTACTACAATCAAGCAAAAGTCCTAAGAAAGATGAAATCA2740.13026280759137412No Hit
GCTTACAACATTGCCAGCAGATCCGCCTCCTGCTTCCACCAATCGGCGGT2740.13026280759137412No Hit
AACAAGAAACACACCGCCTGTAACACGAGCAGGGGTCCTAGGAGTCCTGA2730.12978739588483626No Hit
CTCATAAGGTGGGAAATTTTACGGGACTCTATTCCTCTACTGTTCCTACT2720.12931198417829842No Hit
GTCCAAGAGTTATCCAAAATATTTAGGTAAGTAAAATTTCTTCGAGAAAT2710.12883657247176056No Hit
GGCCTGGCCGTTGCCGAGCAACGGGGTAAAGGTTCATGTATTGTTGACAT2690.12788574905868483No Hit
GTTATACAGGCATACAGAGGCATGAGAGCAGGATAACCACATTGAGTAAA2640.12550869052599553No Hit
GTTGGTGGGACTGTAAACTAGTTCAACCATTGTGGAAGTCAGTGTGGAGA2620.12455786711291977No Hit
GGATAACCACATTGAGTAAAGGGGGCGGCAAAGCCCAAAAGACCCACAAT2590.1231316319933062No Hit
TTTAAATACCTCGAAAACAAAAAGATGGGGTTATACCCTAAATTTCATGG2580.12265622028676834No Hit
CTATAAATCAGGCTGATATAAAGACACATACACATGTATGTTTATTGCGG2550.12122998516715475No Hit
GTCTTAAAATAATGATTAACCACGTTATCAGGATAATAGGGTTTAATTCC2550.12122998516715475No Hit
CCCATAGGTATTTTCCTAAAGCCCAGGATGATGGGATGGGAATACAAGTG2510.11932833834100331No Hit
GCATAGTATTCCATGGTGTATATGTGCCACATTTTCTTAATCCAATCTAT2490.11837751492792757No Hit
GGGTTATACCCTAAATTTCATGGGTTATGTTATTGGCAGTTGGGGATCAT2480.11790210322138973No Hit
ATACCAGATCATCCATATAACTAAAAGCCAGACAGTGGGGGAAAGCCCTA2480.11790210322138973No Hit
CCCTGTGCCTGTGGACTCCACCCCAACAGGCCACCGTGTGGGGGTGTGAA2460.116951279808314No Hit
CTCCAAAAGACACCAAATACTCAAGAACTGTTTCTCTTCCAAAAGTAAGG2460.116951279808314No Hit
ACCCAGGACCGTATCCTCCCACTCCTACCTTGTTTGCCATTGGCCAATTG2450.11647586810177614No Hit
GATATAAAGACACATACACATGTATGTTTATTGCGGCACTATTCACAATA2430.11552504468870042No Hit
GAACAAATTGCACTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCAC2420.11504963298216254No Hit
CCCCCACACGGTGGCCTGTTGGGGTGGAGTCCACAGGCACAGGGTGTGCT2420.11504963298216254No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG2410.11457422127562468No Hit
CCTCTACTGTTCCTACTTTTAATCCTGACTGGTTAACTCCTTCTTTTCCT2410.11457422127562468No Hit
GTGCTGGTGTACAGCCACTCTGATTAAGCATCTCTTATGGCCAAGTTACA2400.11409880956908683No Hit
GTTGTAAGCACACCCTGTGCCTGTGGACTCCACCCCAACAGGCCACCGTG2390.11362339786254896No Hit
GTTCCTCGTTGACAAAAATCCTCACAATACCACAGAGGCTAGACTCGTGG2390.11362339786254896No Hit
ATACAGCCCCAAGCGACCCCGAGAGGGATCGTCCGCTGGATTCAGCGCCG2360.11219716274293537No Hit
ACCTATGGGAGTGGGCCTCAGCCCGTTTCTCCTGGCTCAGTTTACTAGTG2340.11124633932985965No Hit
GCCAATAACATAACCCATGAAATTTAGGGTATAACCCCATCTTTTTGTTT2340.11124633932985965No Hit
CCTTTACCCCGTTGCTCGGCAACGGCCAGGCCTGTGCCAAGTGTTTGCTG2340.11124633932985965No Hit
GAATAGAGGATCCAGCTGATGGTCTGGAAAGAATCCCAGAGGATTGGGTA2290.10886928079717034No Hit
ACAACAACAAGGACAATTGGCCAATGGCAAACAAGGTAGGAGTGGGAGGA2280.10839386909063248No Hit
CCCCTATCTTATCCACACTTCCGGAAACTACTGTTGTTAGACGACGAGGC2190.10411516373179174No Hit
CAATAGGGCGATTTACAGGAAGTTTACGAAAACAATCTTTGAGTTTTTGT2180.10363975202525386No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGATCCACGACCACC2180.10363975202525386No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG2180.10363975202525386No Hit
TCCACAGGCCATGCAGTGGAACTCCACTCAGTTCCACCAGGCTCTGTTAG2170.10316434031871602No Hit
ATACTAACATGGGCCTAAAAATTAGACAATTGCTGTGGTTTCACATTTCC2150.10221351690564028No Hit
CATATACACCATGGAATACTATGCAGCCATAAAAATGATGAGTTCATGTC2140.10173810519910242No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN2120.10078728178602671No Hit
GTATAACCCCATCTTTTTGTTTTCGAGGTATTTAAATGGATACCCACAGA2120.10078728178602671No Hit
ATGTTAGTATTCCTTGGACTCATAAGGTGGGAAATTTTACGGGACTCTAT2120.10078728178602671No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)