FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002488223

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488223
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences210344
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN29011.379169360666337No Hit
CTCTTATATAGAATGCCTGCTTTCCATAAAGTATGCAAATAGTGTCTGGT13760.6541665081960978No Hit
CTTATATAGAATGCCTGCTTTCCATAAAGTATGCAAATAGTGTCTGGTCT12870.6118548663142281No Hit
GTGTATATGTGCCACATTTTCTTAATCCAATCTATCATTGTTGGACATTT9440.4487886509717415No Hit
ATTCCTCTACTGTTCCTACTTTTAATCCTGACTGGTTAACTCCTTCTTTT6960.3308865477503518No Hit
GGATAATAGGGTTTAATTCCCTTTTCCATAGGAAAATATTTGGTAACCTT6800.323279960445746No Hit
CCTTTATACCGCTGTTACCAATTTTTTGTTATCTGTGGGTATCCATTTAA6710.31900125508690524No Hit
GTATTCCATGGTGTATATGTGCCACATTTTCTTAATCCAATCTATCATTG5880.27954208344426273No Hit
GTATATACCCAAAGGACTATAAATCAGGCTGATATAAAGACACATACACA5630.26765679078081617No Hit
ATATAAGAGAGAATCCACACGTAGCGCCTCATTTTGTGGGTCACCATATT5460.2595747917696725No Hit
GTTTCCGTCCGAGGGTTTGGAACAGCAGCAAGAGGGAAACATAGAGGTTC5170.24578785228007455No Hit
GGCATACAGAGGCATGAGAGCAGGATAACCACATTGAGTAAAGGGGGCGG5130.24388620545392312No Hit
CCTCCTGCTTATAGACCACCAAATGCCCCTATCTTATCCACACTTCCGGA5060.24055832350815806No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAATACAAAGATC4710.22391891377933293No Hit
ACAAGAAACACACCGCCTGTAACACGAGCAGGGGTCCTAGGAGTCCTGAT4390.20870573917012133No Hit
CAAGAAACACACCGCCTGTAACACGAGCAGGGGTCCTAGGAGTCCTGATG4390.20870573917012133No Hit
CTATTATCCTGATAACGTGGTTAATCATTATTTTAAGACCAGACACTATT4270.20300079869166698No Hit
GTCTTTGATGTATTCTTCGATCCAGGTGATTGACCTTCCTGAAAGTCCAA4080.1939679762674476No Hit
CTATTCCTCTACTGTTCCTACTTTTAATCCTGACTGGTTAACTCCTTCTT4060.19301715285437188No Hit
CTATATAAGAGAGAATCCACACGTAGCGCCTCATTTTGTGGGTCACCATA4050.19254174114783404No Hit
CTTTTACACTGTTGGTGGGACTGTAAACTAGTTCAACCATTGTGGAAGTC3840.18255809531053893No Hit
CTTTTAATCCTGACTGGTTAACTCCTTCTTTTCCTGACATTCATTTACAT3740.1778039782451603No Hit
GAGTAAAGGGGGCGGCAAAGCCCAAAAGACCCACAATGCGTTGACAAACT3660.1740006845928574No Hit
GTCCCGTGCTGGTGGTCGTGGATCCTGGAAGTAGAGGACAAACGGGCAAC3560.1692465675274788No Hit
GTATGCAAATAGTGTCTGGTCTTAAAATAATGATTAACCACGTTATCAGG3560.1692465675274788No Hit
GGGTAAAGGTTCATGTATTGTTGACATAGAAAGGCCTTGTAAGTTGGCGA3540.16829574411440307No Hit
ATGCAAATAGTGTCTGGTCTTAAAATAATGATTAACCACGTTATCAGGAT3540.16829574411440307No Hit
GTATAAAGGGACTCAAGATGCTGCACAGATTTGGCCCCCAATACCAGATC3480.1654432738751759No Hit
GTGCTGGTGAGGATGTGGAGAAATGGGAACACTTTTACACTGTTGGTGGG3400.161639980222873No Hit
TGTCTGTGCCGATGAGTTTCGCTCCAGACCGGCTGCGAGCGAAACAAGCT3340.15878750998364585No Hit
TCCCATTACTGGGTATATACCCAAAGGACTATAAATCAGGCTGATATAAA3310.15736127486403226No Hit
CCATAAAGTATGCAAATAGTGTCTGGTCTTAAAATAATGATTAACCACGT3300.1568858631574944No Hit
GCACGGGACCATGCAAAACCTGCACAACTCTTGCTCAAGGAACCTCTATG3240.15403339291826723No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGGG3210.15260715779865364No Hit
CTTCCCAATCAATAGGGCGATTTACAGGAAGTTTACGAAAACAATCTTTG2990.14214810025482066No Hit
ATCTGGTATTGGGGGCCAAATCTGTGCAGCATCTTGAGTCCCTTTATACC2850.1354923363632906No Hit
CTGTATGCCTGTATAACTGCTAAACAGGCTTTTGTCTTTTCGCCAACTTA2850.1354923363632906No Hit
GTCTTAAAATAATGATTAACCACGTTATCAGGATAATAGGGTTTAATTCC2800.13311527783060131No Hit
TCCTTGGACTCATAAGGTGGGAAATTTTACGGGACTCTATTCCTCTACTG2760.13121363100444985No Hit
AGTCTAGACTCCGTGGTATTGTGAGGATTTTTGTCAACAAGAAACACACC2730.12978739588483626No Hit
ACTCTATTCCTCTACTGTTCCTACTTTTAATCCTGACTGGTTAACTCCTT2710.12883657247176056No Hit
GATTAAAATTAGTTATGCCATCCAGATTTTTTCCTAAGGTTACCAAATAT2700.12836116076522266No Hit
GTCCAAGAGTTATCCAAAATATTTAGGTAAGTAAAATTTCTTCGAGAAAT2660.12645951393907123No Hit
CTATAAATCAGGCTGATATAAAGACACATACACATGTATGTTTATTGCGG2650.12598410223253337No Hit
GAATAGAGGATCCAGCTGATGGTCTGGAAAGAATCCCAGAGGATTGGGTA2630.12503327881945764No Hit
CCCCCACACGGTGGCCTGTTGGGGTGGAGTCCACAGGCACAGGGTGTGCT2610.12408245540638194No Hit
GCACCGACAACTCTGTTGTCCTCTCTAGGAAGTACACCTCCTTCCCATGG2600.12360704369984406No Hit
GAACAAATTGCACTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCAC2560.12170539687369263No Hit
GTTCCTCGTTGACAAAAATCCTCACAATACCACGGAGTCTAGACTCGTGG2530.12027916175407903No Hit
ATGTGATCTTGTGGTAATGATCCCCAACTGCCAATAACATAACCCATGAA2520.11980375004754117No Hit
ATGTAAGACTGTTGGCAAGCTTCGAGGGGCGTTCACGGTGGTCTCCATGC2510.11932833834100331No Hit
GGTCCTAGGAGTCCTGATGTGATGTTGTCCATGTTCGTAGCAGGGCCCCC2510.11932833834100331No Hit
GATATAAAGACACATACACATGTATGTTTATTGCGGCACTATTCACAATA2500.11885292663446545No Hit
GCTTACAACATTGCCAGCAGATCCGCCTCCTGCTTCCACCAATCGGCGGT2470.11742669151485186No Hit
TTTAAATACCTCGAAAACAAAAAGATGGGGTTATACCCTAAATTTCATGG2460.116951279808314No Hit
GTGCTGGTGTACAGCCACTCTGATTAAGCATCTCTTATGGCCAAGTTACA2440.11600045639523827No Hit
GCCAATAACATAACCCATGAAATTTAGGGTATAACCCCATCTTTTTGTTT2420.11504963298216254No Hit
TTCCCCCACTGTCTGGCTTTTAGTTATATGGATGATCTGGTATTGGGGGC2400.11409880956908683No Hit
GTTATACAGGCATACAGAGGCATGAGAGCAGGATAACCACATTGAGTAAA2380.11314798615601111No Hit
GGCACAGGGTGTGCTTACAACATTGCCAGCAGATCCGCCTCCTGCTTCCA2380.11314798615601111No Hit
GTTTTTGTTGAAGTCCCAGTCGGGACTGCTGGAATTTGCCCTGAATAGAG2370.11267257444947325No Hit
CCTTTACCCCGTTGCTCGGCAACGGCCAGGCCTGTGCCAAGTGTTTGCTG2350.11172175103639753No Hit
CCTCTACTGTTCCTACTTTTAATCCTGACTGGTTAACTCCTTCTTTTCCT2280.10839386909063248No Hit
CTCATAAGGTGGGAAATTTTACGGGACTCTATTCCTCTACTGTTCCTACT2270.10791845738409463No Hit
GCATAGTATTCCATGGTGTATATGTGCCACATTTTCTTAATCCAATCTAT2260.10744304567755676No Hit
GTTAGTATTCCTTGGACTCATAAGGTGGGAAATTTTACGGGACTCTATTC2250.10696763397101891No Hit
GGCCTGGCCGTTGCCGAGCAACGGGGTAAAGGTTCATGTATTGTTGACAT2250.10696763397101891No Hit
CTCCAAAAGACACCAAATACTCAAGAACTGTTTCTCTTCCAAAAGTAAGG2250.10696763397101891No Hit
GTATAACCCCATCTTTTTGTTTTCGAGGTATTTAAATGGATACCCACAGA2230.10601681055794317No Hit
CCCTGTGCCTGTGGACTCCACCCCAACAGGCCACCGTGTGGGGGTGTGAA2230.10601681055794317No Hit
GGATAACCACATTGAGTAAAGGGGGCGGCAAAGCCCAAAAGACCCACAAT2230.10601681055794317No Hit
CCTTTACTCAATGTGGTTATCCTGCTCTCATGCCTCTGTATGCCTGTATA2220.10554139885140532No Hit
GCTATATTGTGCTGGGGTGAGTTAATGACTTTGGCTTCCTGGGTGGGCAA2210.10506598714486745No Hit
GATCTGGTATTGGGGGCCAAATCTGTGCAGCATCTTGAGTCCCTTTATAC2200.1045905754383296No Hit
TCCACAGGCCATGCAGTGGAACTCCACTCAGTTCCACCAGGCTCTGTTAG2190.10411516373179174No Hit
TAGTAAACCTGTACTACAATCAAGCAAAAGTCCTAAGAAAGATGAAATCA2190.10411516373179174No Hit
GTTGTAAGCACACCCTGTGCCTGTGGACTCCACCCCAACAGGCCACCGTG2180.10363975202525386No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCTGATGGCC2170.10316434031871602No Hit
GGGTTATACCCTAAATTTCATGGGTTATGTTATTGGCAGTTGGGGATCAT2150.10221351690564028No Hit
TCCCATGGCTGCTCGGTTGTGCTGCCAACTGGATCCTACGCGGGACGTCC2120.10078728178602671No Hit
GTTGGTGGGACTGTAAACTAGTTCAACCATTGTGGAAGTCAGTGTGGAGA2110.10031187007948884No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)