FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002488224

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488224
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences438964
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATATAGAATACCAGCCTTCCAAAGAGTATGTAAATAATGTCTGGTTT43170.9834519459454534No Hit
ATTAATAGGTGTCAACAATTTGTGGGCCCTCTCACTGTAAATGAAAAGAG32250.7346843932532053No Hit
CTCTTATATAGAATACCAGCCTTCCAAAGAGTATGTAAATAATGTCTGGT21690.4941179686716906No Hit
ATTCTATCCTACTCACACTAAATATTTGCCCTTAGACAAAGGAATTAAAC21200.48295532207652564No Hit
TCCAAAGAGTATGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTACC21150.4818162765055904No Hit
TATTTAGTGTGAGTAGGATAGAATCTAGCAGGCATAATTAATTTCAATCT21150.4818162765055904No Hit
CACTAAATATTTGCCCTTAGACAAAGGAATTAAACCTTATTATCCAGATC20520.4674643023118069No Hit
CTACAGTACCTATCTTTAATCCTGAATGGCAAACTCCTTCCTTTCCTAAG18930.4312426531560675No Hit
TTATTAATAGGTGTCAACAATTTGTGGGCCCTCTCACTGTAAATGAAAAG18710.4262308526439526No Hit
GGATAATAAGGTTTAATTCCTTTGTCTAAGGGCAAATATTTAGTGTGAGT17370.3957044313428892No Hit
GGATAGAATCTAGCAGGCATAATTAATTTCAATCTTCTCTTTTCATTTAC16100.36677267384113504No Hit
GTATGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTACCTGATCTGG15980.36403896447089057No Hit
CCTTTATACCGCTGTTACCAATTTTCTTTTGTCTCTGGGTATACATTTAA15840.360849636872272No Hit
AGTCTGTACAGCATCGTGAGTCCCTTTATACCGCTGTTACCAATTTTCTT15750.35879935484458864No Hit
GTGTAAATGGAGCTGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT14490.33009540645702157No Hit
CCTATTAATAATGTCCTCTTGTAAATGAATCTTAGGAAAGGAAGGAGTTT14350.32690607885840295No Hit
GGATCATATTGTACAAAAGATCAAACACTGTTTTAGAAAACTTCCTGTTA14190.3232611330314103No Hit
ATATGATCCTGTGGCAAAGTTCCCCAACTTCCAATTATGTAACCCATGAA13910.3168824778341732No Hit
ATCCTACTCACACTAAATATTTGCCCTTAGACAAAGGAATTAAACCTTAT13840.3152878140348639No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTATTCCCTAAACTTCAT12940.29478499375803024No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC12590.28681167476148384No Hit
GTCCCGTACTGGTTGTTGTTGATCCTGGAATTAGAGGACAAACGGGCAAC12010.2735987461386355No Hit
ATAGAATACCAGCCTTCCAAAGAGTATGTAAATAATGTCTGGTTTGGAAG11820.26927037296908174No Hit
CTTTAATCCTGAATGGCAAACTCCTTCCTTTCCTAAGATTCATTTACAAG11480.26152486308672235No Hit
GAGTAGGATAGAATCTAGCAGGCATAATTAATTTCAATCTTCTCTTTTCA11400.25970239017322605No Hit
GTACGGGACCATGCAAAACCTGCACGACTCCTGCTCAAGGCAACTCTATG11150.25400716231855003No Hit
GTCTAAGGGCAAATATTTAGTGTGAGTAGGATAGAATCTAGCAGGCATAA11050.25172907117667964No Hit
GGGTATACATTTAAACCCTAACAAAACAAAAAGATGGGGTTATTCCCTAA10970.24990659826318332No Hit
GTATACATGCATACAAAGGCAGTAAGGCAGGATATCCACATTGTGTAAAT10970.24990659826318332No Hit
GTACCTATCTTTAATCCTGAATGGCAAACTCCTTCCTTTCCTAAGATTCA10650.24261670660919804No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATACAAAGACC10570.24079423369570171No Hit
GCTTTATTCCTCTACAGTACCTATCTTTAATCCTGAATGGCAAACTCCTT10520.2396551881247665No Hit
GTCTTTGAAGTAGGCCTCAAGGTCGGTCGTTGACATTGCTGGGAGTCCAA9910.22575883215935705No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGATCACCCGTG9860.22461978658842183No Hit
CCTCCAGCCTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA9630.21938017696211987No Hit
TTCCTCTACAGTACCTATCTTTAATCCTGAATGGCAAACTCCTTCCTTTC9450.21527961290675315No Hit
ATATAGAATACCAGCCTTCCAAAGAGTATGTAAATAATGTCTGGTTTGGA9300.21186247619394757No Hit
TTATTATCCAGATCAGGTAGTTAATCATTACTTCCAAACCAGACATTATT9120.20776191213858083No Hit
ATTTACAAGAGGACATTATTAATAGGTGTCAACAATTTGTGGGCCCTCTC8830.20115544782715664No Hit
GATCTGGATAATAAGGTTTAATTCCTTTGTCTAAGGGCAAATATTTAGTG8660.197282692885977No Hit
CCTCTACAGTACCTATCTTTAATCCTGAATGGCAAACTCCTTCCTTTCCT8600.19591583820085476No Hit
CTGTAAATGAAAAGAGAAGATTGAAATTAATTATGCCTGCTAGATTCTAT8600.19591583820085476No Hit
TATCCACATTGTGTAAATGGAGCTGCAAAGCCCAAAAGACCCACAATTCT8480.19318212883061026No Hit
TTCAAGTACAGTCTCTCTTCCAAAAGTAAGGCAAGATATATGAAACCACA8350.19022061034617874No Hit
TTGACATACTTTCCAATCAATAGGCCTGTTAACAGGAAGTTTTCTAAAAC8270.1883981374326824No Hit
ATATTGTACAAAAGATCAAACACTGTTTTAGAAAACTTCCTGTTAACAGG8040.18315852780638048No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN8000.18224729134963233No Hit
CCCTTAGACAAAGGAATTAAACCTTATTATCCAGATCAGGTAGTTAATCA7970.1815638640070712No Hit
CTCCGAAAGAGACCAAATATTCAAGTACAGTCTCTCTTCCAAAAGTAAGG7800.1776911090658915No Hit
GAATACCAGCCTTCCAAAGAGTATGTAAATAATGTCTGGTTTGGAAGTAA7700.17541301792402111No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACAGTGTTTGATCTTTTGT7600.1731349267821507No Hit
GTGTTTGATCTTTTGTACAATATGATCCTGTGGCAAAGTTCCCCAACTTC7540.17176807209702846No Hit
GAGTATGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTACCTGATCT7460.16994559918353214No Hit
GTTTAATTCCTTTGTCTAAGGGCAAATATTTAGTGTGAGTAGGATAGAAT7240.16493379867141725No Hit
CTATATAAGAGGGAAACCACACGTAGCGCATCATTTTGCGGGTCACCATA7190.16379475310048205No Hit
CTTCCAAAGAGTATGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTA7070.16106104373023758No Hit
GCTAGATTCTATCCTACTCACACTAAATATTTGCCCTTAGACAAAGGAAT7040.16037761638767645No Hit
GTACAATATGATCCTGTGGCAAAGTTCCCCAACTTCCAATTATGTAACCC7020.15992199815930236No Hit
TTCTAAAACAGTGTTTGATCTTTTGTACAATATGATCCTGTGGCAAAGTT6960.15855514347418012No Hit
CTCTCACTGTAAATGAAAAGAGAAGATTGAAATTAATTATGCCTGCTAGA6850.15604924321812266No Hit
GTACAGCATCGTGAGTCCCTTTATACCGCTGTTACCAATTTTCTTTTGTC6730.1533155338478782No Hit
GGGTAAAGGTTCATGTACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGA6700.15263210650531708No Hit
CATTATTAATAGGTGTCAACAATTTGTGGGCCCTCTCACTGTAAATGAAA6590.15012620624925962No Hit
CATGTATACAAGCTAAACAGGCTTTCACTTTCTCGCCAACTTACAAGGCC6540.1489871606783244No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCCGTCAG6410.14602564219389289No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAGCTGAAAGCCAAA6330.1442031692803966No Hit
GTTCATGTACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGA6170.1405582234534039No Hit
ATGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTACCTGATCTGGAT6070.13828013231153352No Hit
ATGTAAGACCTTGGGCAGGATCTGATGGGCGTTCACGGTGGTCTCCATGC5970.1360020411696631No Hit
GGGATCTAGTAGTCAATTATGTTAATACTAACATGGGTTTAAAGATCAGG5940.135318613827102No Hit
GTATAAAGGGACTCACGATGCTGTACAGACTTGGCCCCCAATACCACATC5830.13281271357104454No Hit
CTCATAAGGTGGGAAACTTTACGGGGCTTTATTCCTCTACAGTACCTATC5760.13121804977173526No Hit
CTCCTAGACACCGCCTCAGCTCTGTATCGGGAAGCCTTAGAGTCTCCTGA5690.12962338597242598No Hit
CAGTAAACCCTGCTCCGAATATTGCCTCTCACATCTCGTCAATCTCCGCG5590.1273452948305556No Hit
AATTATGCCTGCTAGATTCTATCCTACTCACACTAAATATTTGCCCTTAG5510.12552282191705927No Hit
GTTAGTATTCCTTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTATTC5490.12506720368868518No Hit
ATTCCTCTACAGTACCTATCTTTAATCCTGAATGGCAAACTCCTTCCTTT5450.12415596723193703No Hit
GAATTAAACCTTATTATCCAGATCAGGTAGTTAATCATTACTTCCAAACC5440.12392815811774999No Hit
ATATAAGAGGGAAACCACACGTAGCGCATCATTTTGCGGGTCACCATATT5410.12324473077518884No Hit
CCATTTACACAATGTGGATATCCTGCCTTACTGCCTTTGTATGCATGTAT5400.12301692166100181No Hit
GAATCTAGCAGGCATAATTAATTTCAATCTTCTCTTTTCATTTACAGTGA5340.12165006697587956No Hit
GGCTGGAGGAGTGCGAATCCACACTCCGAAAGAGACCAAATATTCAAGTA5310.12096663963331844No Hit
GTCTAGGAGATCTCTGACGGAAGGAAAGAAGTCAGAAGGCAAAAACGAGA5310.12096663963331844No Hit
TCCTTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTATTCCTCTACAG5310.12096663963331844No Hit
GTATACATTTAAACCCTAACAAAACAAAAAGATGGGGTTATTCCCTAAAC5300.12073883051913141No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT5240.11937197583400917No Hit
ACAGTACCTATCTTTAATCCTGAATGGCAAACTCCTTCCTTTCCTAAGAT5040.11481579355026837No Hit
GTACATGAACCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAG5020.11436017532189427No Hit
ATATTTGCCCTTAGACAAAGGAATTAAACCTTATTATCCAGATCAGGTAG5000.11390455709352018No Hit
GGTTTAAAGATCAGGCAACTATTGTGGTTTCATATATCTTGCCTTACTTT4970.11322112975095906No Hit
GTAATGATTAACTACCTGATCTGGATAATAAGGTTTAATTCCTTTGTCTA4950.11276551152258499No Hit
ACCTATCTTTAATCCTGAATGGCAAACTCCTTCCTTTCCTAAGATTCATT4940.11253770240839794No Hit
CTTTCGGAGTGTGGATTCGCACTCCTCCAGCCTATAGACCACCAAATGCC4920.11208208418002387No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGACCGGCAGATGAGAAGGCACAG4850.11048742038071459No Hit
GGATATCCACATTGTGTAAATGGAGCTGCAAAGCCCAAAAGACCCACAAT4850.11048742038071459No Hit
CTCTAAGGCTTCCCGATACAGAGCTGAGGCGGTGTCTAGGAGATCTCTGA4790.10912056569559235No Hit
GTTTTAGAAAACTTCCTGTTAACAGGCCTATTGATTGGAAAGTATGTCAA4780.1088927565814053No Hit
GGGTTTACTGTTCCTGAACTGGAGCCACCAGCAGGAAAATACAGACCCCT4750.10820932923884419No Hit
GGACATTATTAATAGGTGTCAACAATTTGTGGGCCCTCTCACTGTAAATG4720.10752590189628307No Hit
ATGTTAATACTAACATGGGTTTAAAGATCAGGCAACTATTGTGGTTTCAT4660.10615904721116083No Hit
GTTTGGAAGTAATGATTAACTACCTGATCTGGATAATAAGGTTTAATTCC4660.10615904721116083No Hit
ACGTGTGGTTTCCCTCTTATATAGAATACCAGCCTTCCAAAGAGTATGTA4660.10615904721116083No Hit
ATCTTTAATCCTGAATGGCAAACTCCTTCCTTTCCTAAGATTCATTTACA4630.10547561986859971No Hit
ATTAATAATGTCCTCTTGTAAATGAATCTTAGGAAAGGAAGGAGTTTGCC4580.10433657429766449No Hit
CTTTTGTACAATATGATCCTGTGGCAAAGTTCCCCAACTTCCAATTATGT4570.10410876518347745No Hit
GCCTTAGAGTCTCCTGAGCATTGCTCACCTCACCATACTGCACTCAGGCA4550.10365314695510337No Hit
GTAAATGAAAAGAGAAGATTGAAATTAATTATGCCTGCTAGATTCTATCC4520.10296971961254225No Hit
TGTTAATACTAACATGGGTTTAAAGATCAGGCAACTATTGTGGTTTCATA4490.10228629226998114No Hit
TCCTCAGGCCATGCAGTGGAATTCCACTGCCTTCCACCAAGCTCTGCAGG4480.10205848315579409No Hit
TTATACCGCTGTTACCAATTTTCTTTTGTCTCTGGGTATACATTTAAACC4480.10205848315579409No Hit
GTTAACAGGAAGTTTTCTAAAACAGTGTTTGATCTTTTGTACAATATGAT4470.10183067404160706No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTCCGCGAGAGGACGACAGAAT4450.10137505581323297No Hit
TCCCGATACAGAGCTGAGGCGGTGTCTAGGAGATCTCTGACGGAAGGAAA4430.1009194375848589No Hit
GACCAGGCCGTTGCCGAGCAACGGGGTAAAGGTTCATGTACTGTTTACTT4420.10069162847067185No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)