FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002488225

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488225
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences438964
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATATAGAATACCAGCCTTCCAAAGAGTATGTAAATAATGTCTGGTTT40670.9264996673986934No Hit
ATTAATAGGTGTCAACAATTTGTGGGCCCTCTCACTGTAAATGAAAAGAG29460.671125650395021No Hit
CTCTTATATAGAATACCAGCCTTCCAAAGAGTATGTAAATAATGTCTGGT23270.530111808713243No Hit
TCCAAAGAGTATGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTACC20470.46632525674087166No Hit
TATTTAGTGTGAGTAGGATAGAATCTAGCAGGCATAATTAATTTCAATCT20190.4599466015436346No Hit
GTATGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTACCTGATCTGG19620.44696148203497327No Hit
TTATTAATAGGTGTCAACAATTTGTGGGCCCTCTCACTGTAAATGAAAAG18930.4312426531560675No Hit
ATTCTATCCTACTCACACTAAATATTTGCCCTTAGACAAAGGAATTAAAC18100.41233449667854316No Hit
GGATAATAAGGTTTAATTCCTTTGTCTAAGGGCAAATATTTAGTGTGAGT17870.40709488705224117No Hit
CACTAAATATTTGCCCTTAGACAAAGGAATTAAACCTTATTATCCAGATC17650.4020830865401263No Hit
CTACAGTACCTATCTTTAATCCTGAATGGCAAACTCCTTCCTTTCCTAAG17580.400488422740817No Hit
CCTTTATACCGCTGTTACCAATTTTCTTTTGTCTCTGGGTATACATTTAA16140.3676839102978832No Hit
AGTCTGTACAGCATCGTGAGTCCCTTTATACCGCTGTTACCAATTTTCTT15540.35401536344666074No Hit
GTGTAAATGGAGCTGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT14800.33715748899681974No Hit
CCTATTAATAATGTCCTCTTGTAAATGAATCTTAGGAAAGGAAGGAGTTT14690.33465158874076234No Hit
GGATAGAATCTAGCAGGCATAATTAATTTCAATCTTCTCTTTTCATTTAC14440.3289563608860863No Hit
GGATCATATTGTACAAAAGATCAAACACTGTTTTAGAAAACTTCCTGTTA14060.32029961454697875No Hit
ATATGATCCTGTGGCAAAGTTCCCCAACTTCCAATTATGTAACCCATGAA13190.30048022161270627No Hit
GTCTAAGGGCAAATATTTAGTGTGAGTAGGATAGAATCTAGCAGGCATAA12290.2799774013358727No Hit
CCTCCAGCCTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA12030.2740543643670096No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTATTCCCTAAACTTCAT11980.2729153187960744No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC11850.26995380031164284No Hit
ATCCTACTCACACTAAATATTTGCCCTTAGACAAAGGAATTAAACCTTAT11830.26949818208326876No Hit
CTTTAATCCTGAATGGCAAACTCCTTCCTTTCCTAAGATTCATTTACAAG11730.26722009094139837No Hit
GTACGGGACCATGCAAAACCTGCACGACTCCTGCTCAAGGCAACTCTATG11690.26630885448465025No Hit
ATAGAATACCAGCCTTCCAAAGAGTATGTAAATAATGTCTGGTTTGGAAG11480.26152486308672235No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGATCACCCGTG10990.2503622164915574No Hit
GGGTATACATTTAAACCCTAACAAAACAAAAAGATGGGGTTATTCCCTAA10630.24216108838082395No Hit
GTACCTATCTTTAATCCTGAATGGCAAACTCCTTCCTTTCCTAAGATTCA10570.24079423369570171No Hit
GTATACATGCATACAAAGGCAGTAAGGCAGGATATCCACATTGTGTAAAT10560.24056642458151464No Hit
GAGTAGGATAGAATCTAGCAGGCATAATTAATTTCAATCTTCTCTTTTCA10470.2385161425538313No Hit
GTCCCGTACTGGTTGTTGTTGATCCTGGAATTAGAGGACAAACGGGCAAC10440.23783271521127017No Hit
ATATAGAATACCAGCCTTCCAAAGAGTATGTAAATAATGTCTGGTTTGGA10140.23099844178565898No Hit
GCTTTATTCCTCTACAGTACCTATCTTTAATCCTGAATGGCAAACTCCTT9940.22644225950191813No Hit
CTCCGAAAGAGACCAAATATTCAAGTACAGTCTCTCTTCCAAAAGTAAGG9890.22530321393098296No Hit
TTCCTCTACAGTACCTATCTTTAATCCTGAATGGCAAACTCCTTCCTTTC9810.22348074101748663No Hit
GTGTTTGATCTTTTGTACAATATGATCCTGTGGCAAAGTTCCCCAACTTC9330.21254590353650868No Hit
CCTCTACAGTACCTATCTTTAATCCTGAATGGCAAACTCCTTCCTTTCCT9260.21095123973719943No Hit
TTATTATCCAGATCAGGTAGTTAATCATTACTTCCAAACCAGACATTATT9030.2057116301108975No Hit
TATCCACATTGTGTAAATGGAGCTGCAAAGCCCAAAAGACCCACAATTCT9030.2057116301108975No Hit
GATCTGGATAATAAGGTTTAATTCCTTTGTCTAAGGGCAAATATTTAGTG8980.20457258453996227No Hit
ATTTACAAGAGGACATTATTAATAGGTGTCAACAATTTGTGGGCCCTCTC8970.20434477542577525No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATACAAAGACC8890.20252230251227893No Hit
CTGTAAATGAAAAGAGAAGATTGAAATTAATTATGCCTGCTAGATTCTAT8730.19887735668528625No Hit
GAGTATGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTACCTGATCT8400.19135965591711393No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN8010.18247510046381935No Hit
GTCTTTGAAGTAGGCCTCAAGGTCGGTCGTTGACATTGCTGGGAGTCCAA7890.17974139109357487No Hit
TTCAAGTACAGTCTCTCTTCCAAAAGTAAGGCAAGATATATGAAACCACA7890.17974139109357487No Hit
GTTTAATTCCTTTGTCTAAGGGCAAATATTTAGTGTGAGTAGGATAGAAT7680.17495739969564703No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCCGTCAG7600.1731349267821507No Hit
CCCTTAGACAAAGGAATTAAACCTTATTATCCAGATCAGGTAGTTAATCA7590.17290711766796366No Hit
CTATATAAGAGGGAAACCACACGTAGCGCATCATTTTGCGGGTCACCATA7590.17290711766796366No Hit
CTTCCAAAGAGTATGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTA7580.1726793085537766No Hit
CTCTCACTGTAAATGAAAAGAGAAGATTGAAATTAATTATGCCTGCTAGA7550.1719958812112155No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAGCTGAAAGCCAAA7420.16903436272678396No Hit
ATATTGTACAAAAGATCAAACACTGTTTTAGAAAACTTCCTGTTAACAGG7370.16789531715584877No Hit
GAATACCAGCCTTCCAAAGAGTATGTAAATAATGTCTGGTTTGGAAGTAA7270.16561722601397835No Hit
GGGTAAAGGTTCATGTACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGA7200.1640225622146691No Hit
GTACAATATGATCCTGTGGCAAAGTTCCCCAACTTCCAATTATGTAACCC7080.1612888528444246No Hit
TTCTAAAACAGTGTTTGATCTTTTGTACAATATGATCCTGTGGCAAAGTT7030.16014980727348938No Hit
ATGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTACCTGATCTGGAT7020.15992199815930236No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACAGTGTTTGATCTTTTGT6800.15491019764718747No Hit
CATTATTAATAGGTGTCAACAATTTGTGGGCCCTCTCACTGTAAATGAAA6580.14989839713507258No Hit
GCTAGATTCTATCCTACTCACACTAAATATTTGCCCTTAGACAAAGGAAT6530.14875935156413736No Hit
GTTCATGTACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGA6500.14807592422157626No Hit
TTGACATACTTTCCAATCAATAGGCCTGTTAACAGGAAGTTTTCTAAAAC6430.14648126042226697No Hit
GTACAGCATCGTGAGTCCCTTTATACCGCTGTTACCAATTTTCTTTTGTC6130.13964698699665576No Hit
GAATTAAACCTTATTATCCAGATCAGGTAGTTAATCATTACTTCCAAACC6000.13668546851222424No Hit
CTCCTAGACACCGCCTCAGCTCTGTATCGGGAAGCCTTAGAGTCTCCTGA5960.1357742320554761No Hit
CCATTTACACAATGTGGATATCCTGCCTTACTGCCTTTGTATGCATGTAT5810.13235709534267046No Hit
CATGTATACAAGCTAAACAGGCTTTCACTTTCTCGCCAACTTACAAGGCC5780.13167366800010935No Hit
GGCTGGAGGAGTGCGAATCCACACTCCGAAAGAGACCAAATATTCAAGTA5760.13121804977173526No Hit
GTATAAAGGGACTCACGATGCTGTACAGACTTGGCCCCCAATACCACATC5720.1303068133149871No Hit
GAATCTAGCAGGCATAATTAATTTCAATCTTCTCTTTTCATTTACAGTGA5610.12780091305892965No Hit
GGATATCCACATTGTGTAAATGGAGCTGCAAAGCCCAAAAGACCCACAAT5530.12597844014543336No Hit
GTTAGTATTCCTTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTATTC5510.12552282191705927No Hit
CTCATAAGGTGGGAAACTTTACGGGGCTTTATTCCTCTACAGTACCTATC5460.12438377634612406No Hit
TCCTTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTATTCCTCTACAG5460.12438377634612406No Hit
ATTCCTCTACAGTACCTATCTTTAATCCTGAATGGCAAACTCCTTCCTTT5450.12415596723193703No Hit
GTAATGATTAACTACCTGATCTGGATAATAAGGTTTAATTCCTTTGTCTA5440.12392815811774999No Hit
ATATAAGAGGGAAACCACACGTAGCGCATCATTTTGCGGGTCACCATATT5240.11937197583400917No Hit
GTACATGAACCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAG5230.11914416671982213No Hit
CAGTAAACCCTGCTCCGAATATTGCCTCTCACATCTCGTCAATCTCCGCG5220.11891635760563508No Hit
GTATACATTTAAACCCTAACAAAACAAAAAGATGGGGTTATTCCCTAAAC5190.11823293026307397No Hit
ATATTTGCCCTTAGACAAAGGAATTAAACCTTATTATCCAGATCAGGTAG5100.11618264823539061No Hit
GGGATCTAGTAGTCAATTATGTTAATACTAACATGGGTTTAAAGATCAGG5040.11481579355026837No Hit
ATCTTTAATCCTGAATGGCAAACTCCTTCCTTTCCTAAGATTCATTTACA5020.11436017532189427No Hit
AATTATGCCTGCTAGATTCTATCCTACTCACACTAAATATTTGCCCTTAG4920.11208208418002387No Hit
GTTTTAGAAAACTTCCTGTTAACAGGCCTATTGATTGGAAAGTATGTCAA4900.1116264659516498No Hit
GTCTCTCTTCCAAAAGTAAGGCAAGATATATGAAACCACAATAGTTGCCT4830.11003180215234051No Hit
GGGTTTACTGTTCCTGAACTGGAGCCACCAGCAGGAAAATACAGACCCCT4810.10957618392396642No Hit
CTTTTGTACAATATGATCCTGTGGCAAAGTTCCCCAACTTCCAATTATGT4740.10798152012465716No Hit
GTTTGGAAGTAATGATTAACTACCTGATCTGGATAATAAGGTTTAATTCC4730.10775371101047011No Hit
GTACTGTAGAGGAATAAAGCCCCGTAAAGTTTCCCACCTTATGAGTCCAA4670.10638685632534788No Hit
CTTTCGGAGTGTGGATTCGCACTCCTCCAGCCTATAGACCACCAAATGCC4660.10615904721116083No Hit
GTCTAGGAGATCTCTGACGGAAGGAAAGAAGTCAGAAGGCAAAAACGAGA4650.10593123809697379No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGA4600.10479219252603857No Hit
ATGTTAATACTAACATGGGTTTAAAGATCAGGCAACTATTGTGGTTTCAT4580.10433657429766449No Hit
GCCTTAGAGTCTCCTGAGCATTGCTCACCTCACCATACTGCACTCAGGCA4580.10433657429766449No Hit
GGACATTATTAATAGGTGTCAACAATTTGTGGGCCCTCTCACTGTAAATG4560.10388095606929042No Hit
ACCTATCTTTAATCCTGAATGGCAAACTCCTTCCTTTCCTAAGATTCATT4550.10365314695510337No Hit
GGTTTAAAGATCAGGCAACTATTGTGGTTTCATATATCTTGCCTTACTTT4480.10205848315579409No Hit
CTCTTGTACATGTCCCACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGC4450.10137505581323297No Hit
ATGTGGATATCCTGCCTTACTGCCTTTGTATGCATGTATACAAGCTAAAC4410.10046381935648481No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)