FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002488228

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488228
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences652441
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN49390.7570033152422977No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG28940.4435650120087487No Hit
ATATAGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC28770.4409594124219662No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGACAAAGGCATTAAACCCTAT24610.3771988578277576No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT23360.35804003733670936No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTCTTTTCA19140.2933598593589305No Hit
CTCTTACAGTTAATGAAAAGAGGAGATTAAAATTAATTATGCCTGCTAGG18580.28477670777894093No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC18380.2817112965003732No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAG17580.26944965138610233No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA17100.26209266431753986No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCGCAA14940.22898622250900846No Hit
CCTAATCTCCTCCCCCAACTCCTCCCAGTCCTTAAACAAACAGTCTTTGA13580.208141425814748No Hit
GGATAATAGGGTTTAATGCCTTTGTCCAAGGGCAAATATTTGGTAAGGTT13570.20798815525081962No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTCTGACATACT13400.20538255566403704No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT13320.20415639115260995No Hit
GTATACATGCATATAAAGGCATTAAGGCAGGATAGCCACATTGTGTAAAA13130.20124425043797065No Hit
GTTTCCGAAAACATTGCTTGATTTTTAGTACAATATGTTCTTGCGGTAAA12600.19312091054976618No Hit
CCTCCTGCTTACAGACCACCAAATGCCCCTATCCTATCAACACTTCCGGA11850.18162561825513726No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTGGACAAAGGCATTAAAC11750.1800929126158534No Hit
CTCCAAAAGATACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA11650.17856020697656952No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC11380.1744219017505031No Hit
GGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTCTTTTCATTAAC10970.1681378086294393No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT10650.16323315058373095No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCCTGT10620.16277333889194578No Hit
CTATTAATAATGTCCTCCTGTAGATGAATGTGAGGAAAGGAGGGAGTTTG10590.16231352720016062No Hit
CTATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATA10570.16200698607230388No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG10030.15373037562017103No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT9950.15250421110874393No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA9850.15097150546946006No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATA9550.1463733885516085No Hit
GAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCA8830.1353379079487647No Hit
TTCGAAAACTGCCTGTAAATAGACCTATTGATTGGAAAGTATGTCAGAGA8800.13487809625697955No Hit
ATACTATATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA8670.13288557892591055No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC8660.13273230836198216No Hit
CTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGA8530.13073979103091313No Hit
GTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAA8460.12966689708341445No Hit
GTCTTTGAAGTACGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA8060.123536074526279No Hit
ATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATATT7990.1224631805787803No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC7900.12108374550342484No Hit
CTATTATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATT7860.12047066324771129No Hit
CTTTTACACAATGTGGCTATCCTGCCTTAATGCCTTTATATGCATGTATA7790.11939776930021259No Hit
GTTTTGAAGTAATGATTAACTGCATGTTCAGGATAATAGGGTTTAATGCC7690.11786506366092872No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC7550.11571927576593133No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGATTTCTTTCCTTCTATTCG7470.11449311125450423No Hit
GATTGTATACATGCATATAAAGGCATTAAGGCAGGATAGCCACATTGTGT7450.11418657012664747No Hit
GTTCAGGATAATAGGGTTTAATGCCTTTGTCCAAGGGCAAATATTTGGTA7440.1140332995627191No Hit
GCTTTGGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG7310.11204078223165007No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACA7200.11035480602843782No Hit
ATGTAAGACCTTGGGCAAGACCCGGTGGGCGTTCACGGTGGTCTCCATGC6970.10682958305808495No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA6960.10667631249415656No Hit
GCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGGATAATAGGGTTTA6950.10652304193022819No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTACTGTGGTTTCACATTTCC6820.10453052459915915No Hit
CCCACGTGTCCTGGCCAAAATTCGCAGTCCCCAACCTCCAATCACTCACC6770.10376417177951722No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC6760.10361090121558884No Hit
CTTTTTACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAA6740.10330436008773207No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA6730.1031510895238037No Hit
GTTTTATTAGGGTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAAT6700.10269127783201852No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT6690.10253800726809015No Hit
GTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTC6660.10207819557630497No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG6610.10131184275666305No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTCACATTCATCTACAGG6570.10069876050094952No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)