FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002488231

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488231
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences569495
Sequences flagged as poor quality0
Sequence length35-151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATATGTTCAGG31940.5608477686371259No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN15210.2670787276446676No Hit
TAGATAAAGGCATCAAACCTTATTATCCTGAACATATAGTTAATCATTAC13550.23793009596221212No Hit
GGATATTATCAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAACG13290.23336464762640585No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATA13200.23178430012554982No Hit
GTGTAAAGGGAGCGGCAAAACCCAGAAGACCCACAATTCTTTGACATACT12950.22739444595650532No Hit
ATTTATAGGAAGTTTCCGAAAACATTGTTTGATTTTTTGTACAATATGTT12190.21404928928261002No Hit
CCTTTCTCTAAGGGCAAATATTTGGTAAGGTTAGGATAGAACCTAGCAGG11920.20930824678004195No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA11470.20140650927576187No Hit
CTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGATCATGCCTGCTAGG11040.1938559601050053No Hit
GAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATATGTTCA10970.19262680093767284No Hit
CCCTTTACACAATGTGGTTACCCAGCATTAATGCCTTTGTATGCATGTAT10920.19174883010386395No Hit
CTCTTACAGTTAACGAAAAAAGAAGATTAAAATTGATCATGCCTGCTAGG10800.18964170010272258No Hit
CATTTAAACCCTAATAAAACCAAACGTTGGGGATACTCCCTTAACTTCAT10700.1878857584351048No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTGATAATATCCTCTTTC10400.18261793343225138No Hit
GTATACATGCATACAAAGGCATTAATGCTGGGTAACCACATTGTGTAAAG10370.18209115093196604No Hit
CTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT9960.17489179009473307No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC9720.17067753009245032No Hit
CTCTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT9700.17032634175892677No Hit
ATATGTTCCTGTGGCAAGGTACCCCAACTTCCAATTACATATCCCATGAA9620.16892158842483254No Hit
TATCAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAACGAAAAAA9470.16628767592340582No Hit
AGTCTGTACAACATCTTGAATCCCTTTTTACCGCTATTACCAATTTTCTT9220.16189782175436132No Hit
CCCAAGTCTCCTGGCCAAAATTTGCAGTCCCCAACCTCCAATCACTCACC8870.15575202591769902No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGAATGGCAAACTCCCTCTTTTC8870.15575202591769902No Hit
ATCCTAACCTTACCAAATATTTGCCCTTAGAGAAAGGCATCAAACCTTAT8770.1539960842500812No Hit
ATCCTAACCTTACCAAATATTTGCCCTTAGATAAAGGCATCAAACCTTAT8450.14837707091370425No Hit
GGTCTATTTATAGGAAGTTTCCGAAAACATTGTTTGATTTTTTGTACAAT8330.14626994091256287No Hit
CTTTCTCTAAGGGCAAATATTTGGTAAGGTTAGGATAGAACCTAGCAGGC8030.14100211590970949No Hit
CTACTGTACCTGTCTTTAATCCTGAATGGCAAACTCCCTCTTTTCCTCAA7890.13854379757504454No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTGCCACAG7680.13485632007304715No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG7660.1345051317395236No Hit
GGAACATATTGTACAAAAAATCAAACAATGTTTTCGGAAACTTCCTATAA7510.13187121923809692No Hit
GTTAAGGGAGTATCCCCAACGTTTGGTTTTATTAGGGTTTAAATGTATAC7500.13169562507133514No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC7420.13029087173724088No Hit
GTACAACATCTTGAATCCCTTTTTACCGCTATTACCAATTTTCTTTTGTC7390.12976408923695554No Hit
CTTTAATCCTGAATGGCAAACTCCCTCTTTTCCTCAAATTCATTTGAAAG7190.12625220590171993No Hit
GTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTC6840.12010641006505764No Hit
ATTATCAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAACGAAAA6640.11659452672982203No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA6610.1160677442295367No Hit
GGTTAGGATAGAACCTAGCAGGCATGATCAATTTTAATCTTCTTTTTTCG6610.1160677442295367No Hit
GGATAATAAGGTTTGATGCCTTTCTCTAAGGGCAAATATTTGGTAAGGTT6540.11483858506220422No Hit
GCTTTATTCTTCTACTGTACCTGTCTTTAATCCTGAATGGCAAACTCCCT6410.11255586089430109No Hit
GTTTGGAAGTAATGATTAACTATATGTTCAGGATAATAAGGTTTGATGCC6370.11185348422725397No Hit
GGTTAGGATAGAACCTAGCAGGCATGATCAATTTTAATCTCCTTTTTTCG6340.11132670172696864No Hit
GCACAGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG6210.1090439775590655No Hit
GTCTTTAATCCTGAATGGCAAACTCCCTCTTTTCCTCAAATTCATTTGAA6180.10851719505878014No Hit
GTCCTGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC6150.1079904125584948No Hit
AGCGAGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATACTCAAGAA6090.10693684755792414No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAA5940.10430293505649742No Hit
CAATAGGTCTATTTATAGGAAGTTTCCGAAAACATTGTTTGATTTTTTGT5920.10395174672297386No Hit
GTTCAGGATAATAAGGTTTGATGCCTTTCTCTAAGGGCAAATATTTGGTA5740.1007910517212618No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)