FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488232

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488232
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences359399
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA17120.47635079674679115No Hit
CTTTTATATAAGATACCCGCCTTCCACAGAGTGTGTAAATAATGTCTCGT16340.4546478982968789No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC15440.42960609239313413No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC14840.4129115551239708No Hit
TCCACAGAGTGTGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACA14150.3937128372644331No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG12110.3369514105492781No Hit
GGATAATAAGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACATT11660.32443050759740566No Hit
CTACTGTACCTTGCTTCAATCCTAAATGGCAAACTCCTTCTTTTCCTGAC11010.30634475888914553No Hit
CCTCCTGCATATAGACCTCCAAATGCCCCTATCTTATCAACACTTCCGGA10750.2991104594058414No Hit
CCTTTATACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA9650.2685038077457088No Hit
GTGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACATGCTCTGGAT9360.26043478139894655No Hit
TTCTTCTACTGTACCTTGCTTCAATCCTAAATGGCAAACTCCTTCTTTTC9250.2573741162329333No Hit
GTGTGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACATGCTCTGG9220.2565393893694751No Hit
GTATAAAGGGACTCAAGATGTTGTACAGACTTGGCCCCCAATACCACATC8620.23984485210031192No Hit
ATATAAAAGAGAGTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATT8510.23678418693429865No Hit
ATATGTTCCTGTGGCAGTGTGCCCCAACTCCCAATTACATATCCCATGAA8310.23121934117791093No Hit
TCCTCAGGCCATGCAGTGGAACTCCACCACTTTCCACCAAACTCTTCAAG8010.22287207254332927No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTAAAA7470.20784698900108237No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATTGATGATGTGGTATTGGGGGC7450.20729050442544358No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGA7410.20617753527416605No Hit
GCACCGGACCATGCAAAACCTGCACAACTCCTGCTCAAGGCACCTCTATG7390.20562105069852726No Hit
TTATTATCCAGAGCATGTAGTTAATCATTACTTCCAGACGAGACATTATT7360.20478632383506912No Hit
CATGTATACAAGCAAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC7160.19922147807868137No Hit
CCCTTACAGTAAATGAACACAGAAGACTAAAATTAATTATGCCTGCTAGG7150.19894323579086196No Hit
CCCTTATCTCCACCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAA6960.1936566323222936No Hit
CCTCTATGTTTCCCTCATGTTGCTGTACAAAACCTACGGACGGAAACTGC6960.1936566323222936No Hit
GCTCAGAATACTGTCTCTGCCATATCGTCAATCTTATCGAAGACTGGGGA6870.1911524517319191No Hit
ATATTAACGTTGACATAGCTGACTACTAATTCCCTGGATGCAGGATCTTC6850.19059596715628035No Hit
GTATCGGGAGGCTTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGG6830.19003948258064157No Hit
CGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCCT6720.18697881741462832No Hit
GTTTACAGGAAGTTTCCTAAAACACGTTTTGATTTTTTGTACAATATGTT6610.18391815224861505No Hit
CCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAACTCCACCACTTT6580.18308342538515687No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT6560.18252694080951812No Hit
TCCTTGGACACATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACTG6490.1805792447947824No Hit
GGAACATATTGTACAAAAAATCAAAACGTGTTTTAGGAAACTTCCTGTAA6440.17918803335568548No Hit
GTCCGGTGCTGGTTGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC6420.17863154878004667No Hit
GCGTAAAAGGGGCGGCAAACCCCAAAAGGCCCACAATTCGTTGACATACT6300.17529264132621403No Hit
ACATAGAGGTGCCTTGAGCAGGAGTTGTGCAGGTTTTGCATGGTCCGGTG6200.17251021844802017No Hit
GCTTTATTCTTCTACTGTACCTTGCTTCAATCCTAAATGGCAAACTCCTT6190.17223197616020078No Hit
ACTGTACCTTGCTTCAATCCTAAATGGCAAACTCCTTCTTTTCCTGACAT6170.171675491584562No Hit
GTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGC6140.17084076472110382No Hit
CTCTTTTATATAAGATACCCGCCTTCCACAGAGTGTGTAAATAATGTCTC6110.17000603785764568No Hit
ACTTGCAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT5930.16499767667689672No Hit
CTCTGTGGAAGGCGGGTATCTTATATAAAAGAGAGTCCACACGTAGCGCC5900.16416294981343854No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCGTCTGACTTCTTTCCTTCTATTCG5870.1633282229499804No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAG5790.1611022846474253No Hit
ATATAAGATACCCGCCTTCCACAGAGTGTGTAAATAATGTCTCGTCTGGA5780.1608240423596059No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATACCGCTGTTACCAATTTTCTT5750.15998931549614773No Hit
GACCAGACCGTTGCCGAGCAACGGGGTAAAGGTTCAGATACTGTTTACTT5690.1583198617692314No Hit
GTTGATAGATGTAATCAATTTGTGGGGCCCCTTACAGTAAATGAACACAG5590.15553743889103755No Hit
CAATAGGCCTGTTTACAGGAAGTTTCCTAAAACACGTTTTGATTTTTTGT5530.15386798516412123No Hit
GCCTAGCAGCCATGGAAATGATGTATACCGGCGGGAGAGGACGACAGAAT5450.15164204686156613No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG5420.15080731999810795No Hit
ATTCTGAGCAGGGCTCACTGTTCCTGAACTGGAGCCACCAGCAGGAAAGT5400.15025083542246917No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCTGCATATAGACCTCCAAATGCC5360.1491378662711916No Hit
GTTGCAAGTATGCCCTGAGCCTGAGGGCTCCACCCCAACAGTCCCCCATG5320.1480248971199141No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT5080.14134708221224876No Hit
ACCTATGGGAGTGGGCCTCAGTCCGTTTCTCTTGGCTCAGTTTACTAGTG5070.1410688399244294No Hit
GTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCCGCCTGTAACACG5040.14023411306097125No Hit
GTACAGGGTCCCCAGTCTTCGATAAGATTGACGATATGGCAGAGACAGTA4980.1385646593340549No Hit
CCCAATTACATATCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTT4980.1385646593340549No Hit
CCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGGTG4950.13772993247059676No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCATCAACAACC4900.1363387210314998No Hit
GTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAA4900.1363387210314998No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCGTCTG4870.13550399416804165No Hit
GGATATCCACATTGCGTAAAAGGGGCGGCAAACCCCAAAAGGCCCACAAT4860.13522575188022226No Hit
TGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGAA4790.13327805586548655No Hit
GCTTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGGCACTCAGGCA4790.13327805586548655No Hit
CCTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAAATACCTATGGGAG4740.1318868444263896No Hit
CTTTTACGCAATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATA4690.1304956329872927No Hit
CACATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACTGTACCTTGC4680.13021739069947327No Hit
TCCTAGTACAAAGACCTTTAACCTAATCTCCTCCCCCAACTCCTCCCACT4650.12938266383601513No Hit
CCTGTAAACAGGCCTATTGATTGGAAAGTATGTCAACGAATTGTGGGCCT4540.12632199867000188No Hit
ATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAAC4510.1254872718065437No Hit
ATCCTAAATGGCAAACTCCTTCTTTTCCTGACATTCATTTGCAGGAGGAC4500.1252090295187243No Hit
CAGTTATATTGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG4490.12493078723090492No Hit
GTACAGACTTGGCCCCCAATACCACATCATCAATATAACTGAAAGCCAGA4480.12465254494308554No Hit
CAGTTGGACCCTGCATTCAAAGCCAACTCAGAAAATCCAGATTGGGACCT4480.12465254494308554No Hit
CATATAAAGGCATTAAAGCAGGATATCCACATTGCGTAAAAGGGGCGGCA4470.12437430265526617No Hit
TACCTTGATAGTCCAGAAGACCCAACAAGAAGATGAGGCATAGCAGCAGG4450.12381781807962737No Hit
GTCCAACTGATGATCGGGGAAGAATCCCAGGGGATTGGGGACAGAAAGAT4450.12381781807962737No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTC4440.12353957579180799No Hit
GGTCCTAGGAGTCCTGATGCGATGTTCTCCATGTTCGGTACAGGGTCCCC4420.12298309121616922No Hit
CCCCAACAGTCCCCCATGGGGAGGGATGAACCCTGGCCCGAATGCTCCCA4410.12270484892834983No Hit
ACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTT4390.12214836435271105No Hit
GTATGGATCGGCAGAGGAGACACAAAGGTTCCACGCACGCGCTGATGGCC4390.12214836435271105No Hit
GGGTAAAGGTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGA4380.12187012206489167No Hit
GTTAGTATTCCTTGGACACATAAGGTGGGAAACTTTACGGGGCTTTATTC4340.12075715291361411No Hit
GTACAACATCTTGAGTCCCTTTATACCGCTGTTACCAATTTTCTTTTGTC4300.11964418376233657No Hit
GTGTTGGGGTGAGTTGATGAATTTAGCCACCTGGGTGGGAAGTAATTTGG4260.11853121461105902No Hit
GAATACTGTCTCTGCCATATCGTCAATCTTATCGAAGACTGGGGACCCTG4260.11853121461105902No Hit
GCTCTGGATAATAAGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTA4250.11825297232323964No Hit
CTGTAAGGGGCCCCACAAATTGATTACATCTATCAACAATGTCCTCCTGC4240.11797473003542025No Hit
CAATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGG4210.11714000317196208No Hit
ATTCTGTGTTGGGGTGAGTTGATGAATTTAGCCACCTGGGTGGGAAGTAA4210.11714000317196208No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA4170.11602703402068452No Hit
CAGTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATT4110.1143575802937682No Hit
CTCTTATGCAAGACCTTGGGCAGGTTCCGGTGGGCGTTCACGGTGGTCTC4110.1143575802937682No Hit
CTGTTGGGGTGGAGCCCTCAGGCTCAGGGCATACTTGCAACTGTGCCAGC4100.11407933800594881No Hit
CATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACTGTACCTTGCTT4070.11324461114249067No Hit
GCATATAGACCTCCAAATGCCCCTATCTTATCAACACTTCCGGAAACTAC4070.11324461114249067No Hit
TATCCACATTGCGTAAAAGGGGCGGCAAACCCCAAAAGGCCCACAATTCG4050.11268812656685188No Hit
TCTATCAACAATGTCCTCCTGCAAATGAATGTCAGGAAAAGAAGGAGTTT4030.11213164199121312No Hit
ACTACATGCTCTGGATAATAAGGTTTGATCCCTTTATCTAAGGGCAAATA4030.11213164199121312No Hit
GTCGTTGACATTGCTGAAAGTCCAAGAGTCCTCTTATGCAAGACCTTGGG4030.11213164199121312No Hit
GTTTTATCCCAATGTTACTAAATATTTGCCCTTAGATAAAGGGATCAAAC4030.11213164199121312No Hit
ATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGG4020.11185339970339372No Hit
ATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA4020.11185339970339372No Hit
CAATTACATATCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTTTG4010.11157515741557432No Hit
GTACAGTAGAAGAATAAAGCCCCGTAAAGTTTCCCACCTTATGTGTCCAA4000.11129691512775494No Hit
GCCTGAGGATGAGTGTCCCTTAGAGGTGGAGATAAGGGAGTAGGCTGCCT4000.11129691512775494No Hit
GGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTATAA3980.11074043055211617No Hit
GTAAATGAACACAGAAGACTAAAATTAATTATGCCTGCTAGGTTTTATCC3940.10962746140083862No Hit
TCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAATAGAAGGAAA3930.10934921911301923No Hit
GTACAACAGGCGGAGAAGCGGGCGGTAGAGCCCCAAGCGGCCCCGGGAGG3900.10851449224956106No Hit
CTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCCGCCTGTAACA3890.10823624996174168No Hit
CTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGGAGAGAAACTGTTCT3860.10740152309828353No Hit
GCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAGGCTTTTACTTT3830.10656679623482536No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC3820.10628855394700597No Hit
CTCTAAAGCCTCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAA3810.10601031165918659No Hit
GTCCAAGGAATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGAC3800.1057320693713672No Hit
GTAGAAGAATAAAGCCCCGTAAAGTTTCCCACCTTATGTGTCCAAGGAAT3790.10545382708354782No Hit
ACAGTATTCTGAGCAGGGCTCACTGTTCCTGAACTGGAGCCACCAGCAGG3750.10434085793227027No Hit
CCTTTACCCCGTTGCTCGGCAACGGTCTGGTCTGTGCCAAGTGTTTGCTG3730.10378437335663147No Hit
GGTCTGGTCTGTGCCAAGTGTTTGCTGACGCAACCCCCACTGGTTGGGGC3720.1035061310688121No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTATAAA3710.1032278887809927No Hit
GTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTATACCGCTGT3710.1032278887809927No Hit
GCTCTGTATCGGGAGGCTTTAGAGTCTCCGGAACATTGTTCACCTCACCA3690.10267140420535395No Hit
CCCTTAACTTCATGGGATATGTAATTGGGAGTTGGGGCACACTGCCACAG3690.10267140420535395No Hit
CAGTAAATGAACACAGAAGACTAAAATTAATTATGCCTGCTAGGTTTTAT3690.10267140420535395No Hit
TCTATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATT3670.10211491962971515No Hit
CCACAATAGTTGTCTGATTTTTAGGCCCATATTAACGTTGACATAGCTGA3630.10100195047843762No Hit
GGATAAAACCTAGCAGGCATAATTAATTTTAGTCTTCTGTGTTCATTTAC3620.10072370819061821No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)