FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488233

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488233
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences359399
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTTATATAAGATACCCGCCTTCCACAGAGTGTGTAAATAATGTCTCGT16830.46828177040002894No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA14550.4048425287772086No Hit
GGATAATAAGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACATT13100.36449739704339745No Hit
TCCACAGAGTGTGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACA12780.35559364383317704No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG12680.3528112209549832No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC12380.34446395232040156No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC12160.338342621988375No Hit
GTGTGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACATGCTCTGG11500.31997863099229545No Hit
CTACTGTACCTTGCTTCAATCCTAAATGGCAAACTCCTTCTTTTCCTGAC9460.26321720427714046No Hit
GTGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACATGCTCTGGAT9440.2626607197015017No Hit
CCTTTATACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA9160.25486993564255883No Hit
CCTCCTGCATATAGACCTCCAAATGCCCCTATCTTATCAACACTTCCGGA8590.23901012523685375No Hit
GTATAAAGGGACTCAAGATGTTGTACAGACTTGGCCCCCAATACCACATC8180.22760219143625887No Hit
ATATAAAAGAGAGTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATT8120.22593273770934252No Hit
TTCTTCTACTGTACCTTGCTTCAATCCTAAATGGCAAACTCCTTCTTTTC8050.2239850416946068No Hit
CCCTTATCTCCACCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAA8020.22315031483114864No Hit
CCTCTATGTTTCCCTCATGTTGCTGTACAAAACCTACGGACGGAAACTGC7450.20729050442544358No Hit
TCCTCAGGCCATGCAGTGGAACTCCACCACTTTCCACCAAACTCTTCAAG7250.20172565866905584No Hit
ATATGTTCCTGTGGCAGTGTGCCCCAACTCCCAATTACATATCCCATGAA7220.20089093180559767No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGA6950.1933783900344742No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT6940.19310014774665485No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTAAAA6820.18976124029282218No Hit
GTTTACAGGAAGTTTCCTAAAACACGTTTTGATTTTTTGTACAATATGTT6770.18837002885372525No Hit
GTATCGGGAGGCTTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGG6660.185309363687712No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATTGATGATGTGGTATTGGGGGC6400.1780750642044079No Hit
CCCTTACAGTAAATGAACACAGAAGACTAAAATTAATTATGCCTGCTAGG6210.17278846073583956No Hit
CCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAACTCCACCACTTT6200.17251021844802017No Hit
ATATTAACGTTGACATAGCTGACTACTAATTCCCTGGATGCAGGATCTTC6170.171675491584562No Hit
TTATTATCCAGAGCATGTAGTTAATCATTACTTCCAGACGAGACATTATT6150.17111900700892324No Hit
GCTCAGAATACTGTCTCTGCCATATCGTCAATCTTATCGAAGACTGGGGA6040.16805834184290996No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCGTCTG6010.16722361497945182No Hit
GCTTTATTCTTCTACTGTACCTTGCTTCAATCCTAAATGGCAAACTCCTT6000.16694537269163243No Hit
ATATAAGATACCCGCCTTCCACAGAGTGTGTAAATAATGTCTCGTCTGGA5960.16583240354035486No Hit
CGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCCT5900.16416294981343854No Hit
CATGTATACAAGCAAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC5870.1633282229499804No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATACCGCTGTTACCAATTTTCTT5860.16304998066216098No Hit
CTCTTTTATATAAGATACCCGCCTTCCACAGAGTGTGTAAATAATGTCTC5820.16193701151088347No Hit
GTCCGGTGCTGGTTGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC5810.16165876922306405No Hit
GGAACATATTGTACAAAAAATCAAAACGTGTTTTAGGAAACTTCCTGTAA5740.15971107320832834No Hit
GCGTAAAAGGGGCGGCAAACCCCAAAAGGCCCACAATTCGTTGACATACT5700.1585981040570508No Hit
CAATAGGCCTGTTTACAGGAAGTTTCCTAAAACACGTTTTGATTTTTTGT5690.1583198617692314No Hit
GCACCGGACCATGCAAAACCTGCACAACTCCTGCTCAAGGCACCTCTATG5690.1583198617692314No Hit
CTCTGTGGAAGGCGGGTATCTTATATAAAAGAGAGTCCACACGTAGCGCC5550.15442446973975998No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAG5530.15386798516412123No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCGTCTGACTTCTTTCCTTCTATTCG5500.15303325830066306No Hit
TCCTTGGACACATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACTG5430.15108556228592734No Hit
ACTTGCAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT5360.1491378662711916No Hit
GACCAGACCGTTGCCGAGCAACGGGGTAAAGGTTCAGATACTGTTTACTT5320.1480248971199141No Hit
ACATAGAGGTGCCTTGAGCAGGAGTTGTGCAGGTTTTGCATGGTCCGGTG5190.14440774737826204No Hit
ACTGTACCTTGCTTCAATCCTAAATGGCAAACTCCTTCTTTTCCTGACAT5180.14412950509044264No Hit
ACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTT5180.14412950509044264No Hit
ATTCTGAGCAGGGCTCACTGTTCCTGAACTGGAGCCACCAGCAGGAAAGT5090.14162532450006818No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA5080.14134708221224876No Hit
GTCCAACTGATGATCGGGGAAGAATCCCAGGGGATTGGGGACAGAAAGAT4880.13578223645586104No Hit
GTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAA4870.13550399416804165No Hit
GTTGATAGATGTAATCAATTTGTGGGGCCCCTTACAGTAAATGAACACAG4860.13522575188022226No Hit
GGGTAAAGGTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGA4780.13299981357766716No Hit
CCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGGTG4740.1318868444263896No Hit
GTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGC4700.13077387527511206No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCTGCATATAGACCTCCAAATGCC4700.13077387527511206No Hit
GTACAACATCTTGAGTCCCTTTATACCGCTGTTACCAATTTTCTTTTGTC4660.12966090612383452No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTC4630.12882617926037634No Hit
GCTCTGGATAATAAGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTA4630.12882617926037634No Hit
ACCTATGGGAGTGGGCCTCAGTCCGTTTCTCTTGGCTCAGTTTACTAGTG4570.12715672553346002No Hit
GTTGCAAGTATGCCCTGAGCCTGAGGGCTCCACCCCAACAGTCCCCCATG4560.12687848324564063No Hit
CAGTTATATTGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG4540.12632199867000188No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT4520.1257655140943631No Hit
CCCTTAACTTCATGGGATATGTAATTGGGAGTTGGGGCACACTGCCACAG4480.12465254494308554No Hit
CTGTAAGGGGCCCCACAAATTGATTACATCTATCAACAATGTCCTCCTGC4480.12465254494308554No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG4440.12353957579180799No Hit
GTATACATCATTTCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTGC4390.12214836435271105No Hit
ACCTTATTATCCAGAGCATGTAGTTAATCATTACTTCCAGACGAGACATT4380.12187012206489167No Hit
GAATACTGTCTCTGCCATATCGTCAATCTTATCGAAGACTGGGGACCCTG4320.12020066833797534No Hit
GCTTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGGCACTCAGGCA4270.11880945689887841No Hit
CCCAATTACATATCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTT4250.11825297232323964No Hit
GTTTTATCCCAATGTTACTAAATATTTGCCCTTAGATAAAGGGATCAAAC4230.11769648774760084No Hit
CCTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAAATACCTATGGGAG4220.11741824545978147No Hit
GGATAAAACCTAGCAGGCATAATTAATTTTAGTCTTCTGTGTTCATTTAC4170.11602703402068452No Hit
TGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGAA4160.11574879173286515No Hit
GGATATCCACATTGCGTAAAAGGGGCGGCAAACCCCAAAAGGCCCACAAT4160.11574879173286515No Hit
ACTACATGCTCTGGATAATAAGGTTTGATCCCTTTATCTAAGGGCAAATA4160.11574879173286515No Hit
GTATGGATCGGCAGAGGAGACACAAAGGTTCCACGCACGCGCTGATGGCC4150.11547054944504576No Hit
GTACAGGGTCCCCAGTCTTCGATAAGATTGACGATATGGCAGAGACAGTA4150.11547054944504576No Hit
GTAGAAGAATAAAGCCCCGTAAAGTTTCCCACCTTATGTGTCCAAGGAAT4140.11519230715722638No Hit
CATATAAAGGCATTAAAGCAGGATATCCACATTGCGTAAAAGGGGCGGCA4120.11463582258158758No Hit
CTTTTACGCAATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATA4100.11407933800594881No Hit
GTACAGTAGAAGAATAAAGCCCCGTAAAGTTTCCCACCTTATGTGTCCAA4040.1124098842790325No Hit
ATCCTAAATGGCAAACTCCTTCTTTTCCTGACATTCATTTGCAGGAGGAC4040.1124098842790325No Hit
CCCCAACAGTCCCCCATGGGGAGGGATGAACCCTGGCCCGAATGCTCCCA4030.11213164199121312No Hit
GCCTGAGGATGAGTGTCCCTTAGAGGTGGAGATAAGGGAGTAGGCTGCCT4020.11185339970339372No Hit
GCTCTGTATCGGGAGGCTTTAGAGTCTCCGGAACATTGTTCACCTCACCA4010.11157515741557432No Hit
ATTCTGTGTTGGGGTGAGTTGATGAATTTAGCCACCTGGGTGGGAAGTAA4000.11129691512775494No Hit
ATCTAAGGGCAAATATTTAGTAACATTGGGATAAAACCTAGCAGGCATAA3990.11101867283993555No Hit
GCTTTGGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG3940.10962746140083862No Hit
CAGTTGGACCCTGCATTCAAAGCCAACTCAGAAAATCCAGATTGGGACCT3930.10934921911301923No Hit
GTACAACAGGCGGAGAAGCGGGCGGTAGAGCCCCAAGCGGCCCCGGGAGG3910.10879273453738046No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCATCAACAACC3890.10823624996174168No Hit
CCTGTAAACAGGCCTATTGATTGGAAAGTATGTCAACGAATTGTGGGCCT3880.1079580076739223No Hit
CTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCCGCCTGTAACA3860.10740152309828353No Hit
CAATTACATATCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTTTG3860.10740152309828353No Hit
ATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAAC3840.10684503852264474No Hit
CCTTTACCCCGTTGCTCGGCAACGGTCTGGTCTGTGCCAAGTGTTTGCTG3840.10684503852264474No Hit
GTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCCGCCTGTAACACG3840.10684503852264474No Hit
TCTATCAACAATGTCCTCCTGCAAATGAATGTCAGGAAAAGAAGGAGTTT3810.10601031165918659No Hit
GTCCAAGGAATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGAC3810.10601031165918659No Hit
CTGTTGGGGTGGAGCCCTCAGGCTCAGGGCATACTTGCAACTGTGCCAGC3800.1057320693713672No Hit
GGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTATAA3720.1035061310688121No Hit
CAGTAAATGAACACAGAAGACTAAAATTAATTATGCCTGCTAGGTTTTAT3720.1035061310688121No Hit
CTTTATCTAAGGGCAAATATTTAGTAACATTGGGATAAAACCTAGCAGGC3690.10267140420535395No Hit
GTTATATTGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG3680.10239316191753456No Hit
GGTCCTAGGAGTCCTGATGCGATGTTCTCCATGTTCGGTACAGGGTCCCC3660.10183667734189578No Hit
CTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGGAGAGAAACTGTTCT3660.10183667734189578No Hit
GTGTTGGGGTGAGTTGATGAATTTAGCCACCTGGGTGGGAAGTAATTTGG3650.10155843505407638No Hit
CCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGACCCGTAT3650.10155843505407638No Hit
GCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAGGCTTTTACTTT3640.10128019276625701No Hit
GAATTAGTAGTCAGCTATGTCAACGTTAATATGGGCCTAAAAATCAGACA3610.10044546590279883No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTATAAA3600.10016722361497946No Hit
GGTCTGGTCTGTGCCAAGTGTTTGCTGACGCAACCCCCACTGGTTGGGGC3600.10016722361497946No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)