FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488236

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488236
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences342953
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG17650.5146477797249186No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT13790.4020959140173726No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG12730.3711878887194455No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA11640.3394051079885582No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG11240.32774170221575555No Hit
TGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTG10990.32045207360775385No Hit
GTATACATGCATATAAAGGCATTAAGGCTGGATAGCCACATTGTGTAAAA10420.30383172038151No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC10370.30237379465990966No Hit
GTCTATTTACAGGCAGTTTCCGAAAACATTGCTTGATTTTTAATACAATA9990.2912935591757471No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAAATC9850.2872113671552662No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCTTGT9070.2644677258983009No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT8790.25630334185733905No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG8540.24901371324933738No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA8500.2478473726720571No Hit
CTTTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT8390.24463993608453638No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC8180.23851664805381495No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA7770.22656165713669218No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT7730.22539531655941195No Hit
ATCCAGCCTTAATGCCTTTATATGCATGTATACAATCTAAGCAGGCTTTC7040.20527594160132728No Hit
GTTTCCGTCCGAAGGTTTTGTACAGCAACAAGAGGGAAACATAGAGGTTC7030.20498435645700722No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA6880.2006105792922062No Hit
TGCAGGACCTGCACGATTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTG6700.195362046694445No Hit
CTATTAATAATGTCCTCTTGTAAATGAATGTTAGGAAAGGAGGGAGTTTG6680.19477887640580488No Hit
CTTTTTACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAA6620.19302936553988448No Hit
ATTGACAACAGTGCCAGCAGCGCCTCCTCCTGCCTCCACCAATCGGCAGT6580.1918630249626042No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAAC6550.190988269529644No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC6310.18399022606596238No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN6300.18369864092164231No Hit
GAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCA6190.18049120433412158No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTGAGA6170.17990803404548145No Hit
AGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCC6070.17699218260228078No Hit
GGTCCTGCATGGTCCCGTGCTGGTGGTTGATGTTCCTGGAAGTAGAGGAC5990.17465950144772024No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG5960.17378474601476004No Hit
GGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTT5850.1705773094272393No Hit
GGGTAAAGGTGCAGGTATTGTTGACACAGAAAGGCCTTGTAAGTTGGCGA5760.1679530431283587No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT5760.1679530431283587No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA5660.165037191685158No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAACATTCATTTACAAG5650.16474560654083795No Hit
TTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT5640.16445402139651788No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC5580.16270451053059748No Hit
CTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTG5550.16182975509763728No Hit
GTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAG5480.1597886590873968No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTG5450.1589139036544366No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG5450.1589139036544366No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG5430.15833073336579648No Hit
GACGCAAACTGCACTTGTATTCCCATCCCATCATCCTGGGCTTTCGCAAG5350.15599805221123594No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG5320.15512329677827574No Hit
CTTTATACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAA5210.151915860190755No Hit
GCCATGGGAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGTTGTCAGTC5100.14870842360323427No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAAC5090.14841683845891418No Hit
GCACGGGACCATGCAGGACCTGCACGATTCCTGCTCAAGGAACCTCTATG5030.14666732759299378No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATA5010.14608415730435365No Hit
CTCTAACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG5000.1457925721600336No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG4860.14171038013955264No Hit
ATGCAGGACCTGCACGATTCCTGCTCAAGGAACCTCTATGTTTCCCTCTT4780.1393776989849921No Hit
GGTAAAAAGGGACTCAAGATGTTGTACAGACTTGGCCCCCAATACCACAT4770.13908611384067204No Hit
GGAACATATTGTATTAAAAATCAAGCAATGTTTTCGGAAACTGCCTGTAA4770.13908611384067204No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA4670.13617026239747138No Hit
GATCATTAACCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAAC4630.1350039218201911No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGAAT4550.13267124066563057No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAAAGAGAAACTACA4530.13208807037699044No Hit
ATATAAAAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATATT4510.13150490008835028No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC4490.13092172979971017No Hit
GTTTTATTAGGGTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAAT4490.13092172979971017No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC4460.13004697436674997No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTC4430.12917221893378977No Hit
GGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC4360.1271311229235493No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACATTCCACCAAGCTCTGCTAG4320.125964782346269No Hit
CTTTTACACAATGTGGCTATCCAGCCTTAATGCCTTTATATGCATGTATA4320.125964782346269No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA4300.12538161205762888No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC4250.12392368633602854No Hit
GCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC4220.12304893090306834No Hit
GGTCAGGTCTCTGCCAAGTGTTTGCTGATGCAACCCCCACTGGATGGGGC4200.12246576061442822No Hit
GGTCCTGCATGGTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGAC4200.12246576061442822No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCCGCTTACAGACCACCAAATGCC4190.12217417547010816No Hit
GTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTAT4190.12217417547010816No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG4090.11925832402690748No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA4060.11838356859394727No Hit
GTATACATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAAC4050.11809198344962721No Hit
TTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTCCGAAAAC4040.11780039830530714No Hit
CTTGTATTCCCATCCCATCATCCTGGGCTTTCGCAAGATTCCTATGGGAG4040.11780039830530714No Hit
GTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAAAGGAGATTAAAA4040.11780039830530714No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC4020.117217228016667No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCCGATGGCC4000.11663405772802687No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG3990.11634247258370681No Hit
GACCTGACCGTTGCCGGGCAACGGGGTAAAGGTGCAGGTATTGTTGACAC3980.11605088743938674No Hit
GTAAACAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCTTTT3960.11546771715074661No Hit
GTTTTGAAGTAATGATTAACTGCATGTTCAGGATAATAAGGTTTAATGCC3940.11488454686210646No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG3920.11430137657346634No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAA3910.11400979142914627No Hit
GTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAA3880.11313503599618606No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGCAGGGCTTTCCCCCACTGTT3820.11138552513026566No Hit
TTTACATACTCTGTGGAAGGCTGGCATTCTATATAAAAGAGAAACTACAC3810.11109393998594559No Hit
GTTCCATCCTACCACGGGGCGCACCTCTCTTTACGCGGTCTCCCCGTTTG3810.11109393998594559No Hit
TTATTATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATT3800.11080235484162552No Hit
CTCCATGTTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGG3800.11080235484162552No Hit
TCCCATGGCTGCTCGGGTGTGCTGCCAACTGGATCCTGCGCGGGACGTCC3780.1102191845529854No Hit
GTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTC3750.10934442912002519No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG3690.10759491825410479No Hit
GTCCGGCAGATGAGAAGGCACAAACGGGGAGACCGCGTAAAGAGAGGTGC3680.10730333310978472No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC3570.10409589652226399No Hit
TCCCGATACAGAGCAGATGCGGTGTCGAGGAGATCTCGAATAGAAGGAAA3550.10351272623362386No Hit
GTCCCGTGCTGGTGGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC3530.10292955594498371No Hit
CTCTAAGGCCTCCCGATACAGAGCAGATGCGGTGTCGAGGAGATCTCGAA3510.10234638565634359No Hit
GGATGGGGCTTGGCCATAGGCCATCGGCGCATGCGTGGAACCTTTGTGGC3500.10205480051202352No Hit
ATACAGCACTCAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTG3500.10205480051202352No Hit
GGTCTATTTACAGGCAGTTTCCGAAAACATTGCTTGATTTTTAATACAAT3490.10176321536770346No Hit
GCCTGAGGATGACTGTCTCTTAGAGGTGGAGAGATGGGAGTAGGCTGTCT3480.10147163022338337No Hit
AGGCTGGATAGCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCC3480.10147163022338337No Hit
GCCTATGGCCAAGCCCCATCCAGTGGGGGTTGCATCAGCAAACACTTGGC3460.10088845993474324No Hit
GACCAGACCGTTGCCGGGCAACGGGGTAAAGGTGCAGGTATTGTTGACAC3450.10059687479042317No Hit
CTCTGGGATCTAGCAGAGCTTGGTGGAATGTTGTGGAGTTCCACTGCATG3450.10059687479042317No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)