FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488238

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488238
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences401587
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA30100.7495262545849343No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATACAAAGATC19050.47436794517750824No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN12510.31151406793546604No Hit
CATTTACACCAAGACATTATCAAAAGATGTGAACAATTTGTAGGCCCACT12180.3032966704599501No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC10930.2721701648708748No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA10680.26594486375305976No Hit
GTGTAAAAGGAGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT10140.2524982133385792No Hit
ACACTACAGAGAGTCCAAGAGTCCTCTTATGCAAGACCTTGGGCAAGAAT9290.23133218953800797No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACATAGCGCC9280.23108317749329535No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG9150.22784602091203152No Hit
ACACAAGTCTCCGGAAGTGTTGATAAGATAGGGGCATTTGGTGGTCTATA8800.21913059934709042No Hit
ACAGTAGTCTCCGGAAGTGTTGATAAGATAGGGGCATTTGGTGGTCTATA8390.2089211055138737No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTTTACATTTCAT8370.20842308142444851No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT8210.20443888870904686No Hit
CCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG8200.20418987666433425No Hit
GGATAAGGGTATTAAACCTTATTATCCGGAACATCTAGTTAATCATTACT7850.19547445509939315No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT7760.19323334669697972No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCCGGAT7270.1810317565060622No Hit
CCTTTTACACAATGTGGGTATCCTGCTTTAATGCCCTTGTATGCCTGCAT7100.17679855174594794No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT7010.17455744334353454No Hit
GTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC6990.1740594192541093No Hit
GACTACTGTTGTTAGACGACGAGGCAGGTCCCCTAGAAGAAGAACTCCCT6930.1725653469858337No Hit
GTCCAAGAGTCCTCTTATGCAAGACCTTGGGCAAGAATTGGTGGGCGTTC6910.1720673228964085No Hit
GACTTGTGTTGTTAGACGACGAGGCAGGTCCCCTAGAAGAAGAACTCCCT6850.1705732506281329No Hit
GTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAAC6780.16883016631514466No Hit
TCACCATACTGCACTCAGGCAAGCAATTCTTTGCTGGGGGGAACTAATGA6680.16634004586801862No Hit
ATACTGCACTCAGGCAAGCAATTCTTTGCTGGGGGGAACTAATGACTCTA6560.1633519013314674No Hit
ATCCAACATCTAGGGACCTAGTAGTCAGTTATGTTAACACTCATATGGGC6550.1631028892867548No Hit
CTACAAACCTTGCCAGCAAATCCGCCTCCTGCTTCTACCAATCGCCAGTC6430.16011474475020357No Hit
TACCCACATTGTGTAAAAGGAGCAGCAAAACCCAAAAGACCCACAATACG6410.15961672066077834No Hit
ATATAGAGCTGAGGCGGTATCTAGAAGATCTCGTACTGAAGGAAAGAAGT6390.15911869657135316No Hit
TCTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCAGTACG6210.1546364797665263No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGAACAACAGAGT6210.1546364797665263No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTGAGTTTCTGT6170.1536404315876759No Hit
ATGTTGGATCGTCCAAATTACCACCCACCCAGGTGGCTAGAGTCATTAGT6120.15239537136411288No Hit
GTATTAAACCTTATTATCCGGAACATCTAGTTAATCATTACTTCCAAACC5920.14741513046986082No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACACTACAGAGAGTCCAA5910.14716611842514823No Hit
CACCAAGACATTATCAAAAGATGTGAACAATTTGTAGGCCCACTCACAGT5880.14641908229101042No Hit
CTGTAATGTCAACGACCGACCTTGAGGCATACTTCAAAGACTGTTTGTTT5700.14193686548618356No Hit
GTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGACATG5690.14168785344147097No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA5680.14143884139675836No Hit
CCCCTACTCCGTCTGCCGTTTCGACCGACCACGGGGCGCACCTCTCTTTA5630.14019378117319534No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC5610.13969575708377013No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC5610.13969575708377013No Hit
GACACTACAGAGAGTCCAAGAGTCCTCTTATGCAAGACCTTGGGCAAGAA5590.13919773299434493No Hit
TTCTTCTACTGTTCCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTC5530.13770366072606932No Hit
CTGTAGTGTCAACGACCGACCTTGAGGCATACTTCAAAGACTGTTTGTTT5480.13645860050250633No Hit
ATTTATGCCTACAGCCTCCTAATACAAAGATCATTAATCTAGTCTCCTCC5480.13645860050250633No Hit
AGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTCTACGA5350.13322144392124247No Hit
CCATATACTTGGGAACAAGAGCTACAGCATGGGGCAGAATCTTTCCACCA5310.13222539574239206No Hit
ATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGA5280.13147835960825424No Hit
ACATTACAGAGAGTCCAAGAGTCCTCTTATGCAAGACCTTGGGCAAGAAT5190.12923725120584081No Hit
CCTCTGCACGTCGCATGGAGACCACCGTGAACGCCCACCAATTCTTGCCC5180.12898823916112823No Hit
CTCTACGACCGTTTCTCTTCCAAAAGTGAGACAAGAAACGTGAAACCACA5170.12873922711641564No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC5160.128490215071703No Hit
CTTTGGGACATGGACATTGATCCTTATAAAGAATTTGGAGCTTCTGTGGA5160.128490215071703No Hit
ATCTAGAAGATCTCGTACTGAAGGAAAGAAGTCAGAAGGCAAAAACGAGA5100.12699614280342741No Hit
ATATAAGAGAGAAACAACACATAGCGCCTCATTTTGCGGGTCACCATATA5040.12550207053515178No Hit
GCTCTATATCGGGAAGCCTTAGAGTCTCCTGAGCATTGTTCACATCACCA5030.1252530584904392No Hit
ATCCAAATGTTACCAAATATTTGCCATTGGATAAGGGTATTAAACCTTAT4960.12350997417745095No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTACGCGGGACGTCCTTTGTTTA4950.12326096213273836No Hit
GGCATACAAGGGCATTAAAGCAGGATACCCACATTGTGTAAAAGGAGCAG4950.12326096213273836No Hit
GTATGGATCGGCAGAGGAGCCGGAAAGGTTCCACGCATGCGCTGATGGCC4950.12326096213273836No Hit
TCCTAATACAAAGATCATTAATCTAGTCTCCTCCCCCAACTCCTCCCAGT4860.12101985373032494No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG4820.12002380555147453No Hit
GAGTAGGGGACGAGAGAGTCCCAAGCGGCCCCGAGAAGGGTCGTCCGCGG4810.11977479350676191No Hit
ATCAAAAGATGTGAACAATTTGTAGGCCCACTCACAGTTAACGAGAAAAG4800.11952578146204931No Hit
CTCTAAGGCTTCCCGATATAGAGCTGAGGCGGTATCTAGAAGATCTCGTA4790.11927676941733673No Hit
ATGTGGGTATCCTGCTTTAATGCCCTTGTATGCCTGCATTCAATCTAAGC4710.1172846730596359No Hit
CTCTTATGCAAGACCTTGGGCAAGAATTGGTGGGCGTTCACGGTGGTCTC4660.11603961283607289No Hit
GCCTTAGAGTCTCCTGAGCATTGTTCACATCACCATACTGCACTCAGGCA4640.11554158874664767No Hit
TAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGT4630.11529257670193507No Hit
GCTTTGGGACATGGACATTGATCCTTATAAAGAATTTGGAGCTTCTGTGG4590.11429652852308467No Hit
GACGGGGAGTCCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGTCGAAACGG4590.11429652852308467No Hit
TCCTTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACTG4560.11354949238894685No Hit
CTTCTAGATACCGCCTCAGCTCTATATCGGGAAGCCTTAGAGTCTCCTGA4550.11330048034423426No Hit
ATGCAAGACCTTGGGCAAGAATTGGTGGGCGTTCACGGTGGTCTCCATGC4490.11180640807595862No Hit
GTGTAAACAATACCTGAACCTTTACCCCGTTGCCCGGCAACGGCCAGGTC4430.11031233580768303No Hit
CTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACTGTTCCTGTC4430.11031233580768303No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA4420.1100633237629704No Hit
GACCAATTTATGCCTACAGCCTCCTAATACAAAGATCATTAATCTAGTCT4360.1085692514946948No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG4340.10807122740526959No Hit
GTGTAAATGTATATTAGGAAAAGAGGGTGTTTTCCAATGAGGGTTAAAGA4340.10807122740526959No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT4330.10782221536055699No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA4290.10682616718170658No Hit
GGTTTGTAGTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCTCCGT4180.10408703468986795No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGATCCCGTG4170.10383802264515535No Hit
GCTTTATTCTTCTACTGTTCCTGTCTTTAACCCTCATTGGAAAACACCCT4150.10333999855573013No Hit
GTCGTAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC4140.10309098651101753No Hit
GGATACCCACATTGTGTAAAAGGAGCAGCAAAACCCAAAAGACCCACAAT4120.10259296242159234No Hit
CCTCTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGC4060.10109889015331672No Hit
CCTTTACCCCGTTGCCCGGCAACGGCCAGGTCTGTGCCAAGTGTTTGCTG4050.10084987810860412No Hit
ACCATATACTTGGGAACAAGAGCTACAGCATGGGGCAGAATCTTTCCACC4020.1001028419744663No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)