FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488240

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488240
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences359603
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG20220.5622867439926807No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTCAC18630.5180713175362831No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT16650.46301059779812737No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC14950.4157362424673876No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGACAAAGGCATTAAACCATAT14100.3920990648020177No Hit
GTTACATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG13720.38153185596338185No Hit
AGCAAGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGAA12430.34565896280064407No Hit
CCTCTTGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA11740.32647113622522617No Hit
TAATCATGCCTGCTAGGTTCTATCCTAACCTTACCAAATATTTGCCCTTG11030.3067271407635643No Hit
CCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAA10530.29282291860746434No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN9840.27363509203204645No Hit
AAGCAGTAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGA9680.26918574094209446No Hit
GTATACATGCATATAAAGGCATTAAGGCAGGATAGCCACATTGTGTAAAA9570.26612681206775246No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT8900.24749515437857858No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC8210.22830732780316068No Hit
ATATAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC7880.21913054118013475No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTCTGACATACT7780.21634969674891477No Hit
CCTACTGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA7480.20800716345525483No Hit
CTACGGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTCAC7440.20689482568276682No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG7320.20355781236530285No Hit
TTATTAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAATGAAAAA6960.1935467724129109No Hit
GGATAATATGGTTTAATGCCTTTGTCCAAGGGCAAATATTTGGTAAGGTT6850.1904878435385689No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTGAGGAAAGGAGGGAGTTTG6820.18965359020920294No Hit
ATTCAGGGCCGATGGGACGTAGACAAAGGACGTCCCGCGCAGGATCCAGT6520.18131105691554297No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC6510.18103297247242098No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA6270.174358945837493No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG6210.172690439178761No Hit
GAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCA6200.172412354735639No Hit
GTCTATTTACAGGCAGTTTTCTAAAACATTGCTTGATTTTTTGTACAATA6160.17130001696315103No Hit
GCAAGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGAAC6130.17046576363378502No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTTCCAAAAGTAAGA6030.16768491920256506No Hit
CTATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATA5920.16462599032822306No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT5880.16351365255573508No Hit
GTTTCCGTCCGAAGGTTTTGTACAGCAACAAGAGGGAAACATAGAGGTTC5860.1629574836694911No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTGGACAAAGGCATTAAAC5840.16240131478324707No Hit
GTCCAAGGGATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGAC5770.16045472368139307No Hit
GGGTAAAGGTTCAAATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA5580.15517111926207514No Hit
ACAATATGTAGGCCCTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAA5580.15517111926207514No Hit
GACAAAAACTGCACTTGTATTCCCATCCCATCATCCTGGGCTTTCGCAAG5310.14766283929778115No Hit
CTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATCATT5190.14432582598031718No Hit
CTTATATAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCA5110.1421011504353412No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT5040.1401545593334872No Hit
ATTGACAACAGTGCCAGTAGCACCTCCTTCTGCCTCCACCAATCGGCAGT5030.1398764748903652No Hit
ATATTATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATT4890.13598329268665724No Hit
CTGTAAGAGGGCCTACATATTGTTGACATCTATTAATAATGTCCTCCTGT4790.13320244825543726No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG4780.13292436381231526No Hit
CCTCCTGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA4730.13153394159670526No Hit
CCAAAAGACACCAAATATTCAAGAACAGTTTCTCTTCCAAAAGTAAGACA4450.12374757718928929No Hit
CTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAACCCTAATAAA4420.12291332385992332No Hit
ATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATATT4410.12263523941680131No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA4390.1220790705305573No Hit
GTTCAGGATAATATGGTTTAATGCCTTTGTCCAAGGGCAAATATTTGGTA4390.1220790705305573No Hit
GCTTAGATTGTATACATGCATATAAAGGCATTAAGGCAGGATAGCCACAT4360.1212448172011913No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC4330.12041056387182532No Hit
GCCCAGAATCCCGCGGACGATCCGTCTCGCGGCCGTTTGGGACTCTACCG4310.11985439498558131No Hit
GTCCATGGGATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGAC4290.11929822609933732No Hit
CCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT4240.11790780388372733No Hit
CTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTTCTAAAAC4240.11790780388372733No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTCACATTCATTTACAGG4210.11707355055436133No Hit
GCAGTAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGAAC4200.11679546611123935No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATA4190.11651738166811734No Hit
GGTCTATTTACAGGCAGTTTTCTAAAACATTGCTTGATTTTTTGTACAAT4190.11651738166811734No Hit
GTACCGTAGAAGAATAAAGCCCAGTAAAGTTTCCCACCTTGTGAGTCCAT4150.11540504389562933No Hit
ATTCTGGGCCGATGGGACGTAGACAAAGGACGTCCCGCGCAGGATCCAGT4090.11373653723689735No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACA4060.11290228390753138No Hit
GTCTTATATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT4060.11290228390753138No Hit
GTTTTGAAGTAATGATTAACTGCATGTTCAGGATAATATGGTTTAATGCC4040.11234611502128737No Hit
CTTTTACACAATGTGGCTATCCTGCCTTAATGCCTTTATATGCATGTATA4030.11206803057816536No Hit
CTATTAATTCCCTGGATGCTGGGTCTTCCAAATTACTTCCCACCCAGGTG3970.11039952391943338No Hit
CTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCTTG3960.11012143947631138No Hit
CATATAAAGGCATTAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA3940.10956527059006738No Hit
CAATTACATATCCCATGAAGTTAAGGGAGTAGCCCCAACGTTTGGTTTTA3940.10956527059006738No Hit
CTTTTTACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAA3920.10900910170382337No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA3900.1084529328175794No Hit
CCCATGGACTCACAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACGG3900.1084529328175794No Hit
GTATAGGCCCCTCACTCTGGGGTCTAGCAGAGCTTGGTGGAATGTTGTGG3860.10734059504509139No Hit
GCCCTGAATCCCGCGGACGATCCGTCTCGCGGCCGTTTGGGACTCTACCG3860.10734059504509139No Hit
GCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC3820.1062282572726034No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATCATGCCTGCTAGG3810.1059501728294814No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG3780.1051159195001154No Hit
TATATAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATG3760.10455975061387142No Hit
GAATTAGTAGTCAGCTATGTTAATGTTAATATGGGCCTAAAGATCAGACA3750.10428166617074941No Hit
ATATGGTTTAATGCCTTTGTCCAAGGGCAAATATTTGGTAAGGTTAGGAT3740.10400358172762741No Hit
ATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGGAT3700.1028912439551394No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC3630.10094465285328542No Hit
CAATAGGTCTATTTACAGGCAGTTTTCTAAAACATTGCTTGATTTTTTGT3630.10094465285328542No Hit
GATTGTATACATGCATATAAAGGCATTAAGGCAGGATAGCCACATTGTGT3620.10066656841016344No Hit
GGTTAGGATAGAACCTAGCAGGCATGATTAATTTTAATCTCCTTTTTTCA3610.10038848396704143No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)