FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488241

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488241
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences359603
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG23050.6409846413962064No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTCAC17640.49054095766720524No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT17130.4763586510679833No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC13420.3731893226697219No Hit
GTTACATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG12750.35455766498054797No Hit
CCTCTTGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA11860.3298081495426901No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGACAAAGGCATTAAACCATAT11650.32396837623712815No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT11370.31618201182971223No Hit
AGCAAGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGAA11020.3064490563204423No Hit
CCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAA9980.27752827423575444No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN9740.27085424760082644No Hit
GTATACATGCATATAAAGGCATTAAGGCAGGATAGCCACATTGTGTAAAA9380.26084320764843455No Hit
TAATCATGCCTGCTAGGTTCTATCCTAACCTTACCAAATATTTGCCCTTG9020.25083216769604255No Hit
AAGCAGTAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGA7930.22052096339574473No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG7840.21801820340764674No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC7800.2169058656351588No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTCTGACATACT7750.2155154434195488No Hit
TTATTAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAATGAAAAA7520.20911950122774284No Hit
GGATAATATGGTTTAATGCCTTTGTCCAAGGGCAAATATTTGGTAAGGTT7480.20800716345525483No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTGAGGAAAGGAGGGAGTTTG6870.19104401242481292No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG6850.1904878435385689No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTTCCAAAAGTAAGA6700.18631657689173894No Hit
GTCCAAGGGATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGAC6610.18381381690364096No Hit
GAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCA6460.179642550256811No Hit
GTCTATTTACAGGCAGTTTTCTAAAACATTGCTTGATTTTTTGTACAATA6200.172412354735639No Hit
CTACGGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTCAC6070.16879725697505304No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA6030.16768491920256506No Hit
CCTACTGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA6010.16712875031632105No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC5950.16546024365758907No Hit
GCCCAGAATCCCGCGGACGATCCGTCTCGCGGCCGTTTGGGACTCTACCG5920.16462599032822306No Hit
ATATAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC5900.16406982144197907No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT5860.1629574836694911No Hit
GTTTCCGTCCGAAGGTTTTGTACAGCAACAAGAGGGAAACATAGAGGTTC5760.16017663923827108No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTGGACAAAGGCATTAAAC5710.1587862170226611No Hit
ATTCAGGGCCGATGGGACGTAGACAAAGGACGTCCCGCGCAGGATCCAGT5620.15628345703456312No Hit
CTATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATA5420.15072176817212316No Hit
GCAAGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGAAC5400.15016559928587914No Hit
GTCCATGGGATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGAC5250.14599433263904918No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG5210.14488199486656117No Hit
GGGTAAAGGTTCAAATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA5080.1412668971059752No Hit
GTTCAGGATAATATGGTTTAATGCCTTTGTCCAAGGGCAAATATTTGGTA5040.1401545593334872No Hit
GCCCTGAATCCCGCGGACGATCCGTCTCGCGGCCGTTTGGGACTCTACCG4950.1376517993453892No Hit
ACAATATGTAGGCCCTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAA4930.13709563045914525No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT4900.13626137712977923No Hit
CTGTAAGAGGGCCTACATATTGTTGACATCTATTAATAATGTCCTCCTGT4860.13514903935729125No Hit
ATATTATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATT4820.13403670158480324No Hit
GTACCGTAGAAGAATAAAGCCCAGTAAAGTTTCCCACCTTGTGAGTCCAT4810.13375861714168125No Hit
CTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAACCCTAATAAA4760.13236819492607124No Hit
CTTTTACACAATGTGGCTATCCTGCCTTAATGCCTTTATATGCATGTATA4710.13097777271046127No Hit
CTTTTTACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAA4670.12986543493797326No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA4660.12958735049485126No Hit
GTCTTATATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT4620.12847501272236328No Hit
GTTTTGAAGTAATGATTAACTGCATGTTCAGGATAATATGGTTTAATGCC4550.12652842162050928No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC4500.1251379994048993No Hit
ATTGACAACAGTGCCAGTAGCACCTCCTTCTGCCTCCACCAATCGGCAGT4490.1248599149617773No Hit
GCTTAGATTGTATACATGCATATAAAGGCATTAAGGCAGGATAGCCACAT4350.12096673275806932No Hit
CCTCCTGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA4310.11985439498558131No Hit
CCCTTAACTTCATGGGATATGTAATTGGATGTTGGGGTACTTTACCGCAA4260.11846397276997134No Hit
CTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATCATT4230.11762971944060532No Hit
ATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATATT4230.11762971944060532No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATA4200.11679546611123935No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA4090.11373653723689735No Hit
CCAAAAGACACCAAATATTCAAGAACAGTTTCTCTTCCAAAAGTAAGACA4070.11318036835065336No Hit
GACAAAAACTGCACTTGTATTCCCATCCCATCATCCTGGGCTTTCGCAAG4070.11318036835065336No Hit
CTATTAATTCCCTGGATGCTGGGTCTTCCAAATTACTTCCCACCCAGGTG4070.11318036835065336No Hit
CCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT4060.11290228390753138No Hit
GCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC4050.11262419946440937No Hit
ATATGGTTTAATGCCTTTGTCCAAGGGCAAATATTTGGTAAGGTTAGGAT4050.11262419946440937No Hit
GAATTAGTAGTCAGCTATGTTAATGTTAATATGGGCCTAAAGATCAGACA4040.11234611502128737No Hit
GATTGTATACATGCATATAAAGGCATTAAGGCAGGATAGCCACATTGTGT4010.11151186169192137No Hit
GGTTAGGATAGAACCTAGCAGGCATGATTAATTTTAATCTCCTTTTTTCA3990.11095569280567737No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA3930.10928718614694538No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC3920.10900910170382337No Hit
GGTCTATTTACAGGCAGTTTTCTAAAACATTGCTTGATTTTTTGTACAAT3880.10789676393133539No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACA3870.10761867948821338No Hit
GAATTAGTAGTCAGCTATGTTAATGTTAATATGGGCCTAAAAATCAGACA3830.1065063417157254No Hit
CAATTACATATCCCATGAAGTTAAGGGAGTAGCCCCAACGTTTGGTTTTA3790.1053940039432374No Hit
GTTTGGAAGACCAACCTCCCATGCTGTAGCTCTTGTTCCCAAGAATATGG3770.1048378350569934No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATCATGCCTGCTAGG3740.10400358172762741No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG3730.10372549728450542No Hit
CAATAGGTCTATTTACAGGCAGTTTTCTAAAACATTGCTTGATTTTTTGT3720.10344741284138341No Hit
GTATAGGCCCCTCACTCTGGGGTCTAGCAGAGCTTGGTGGAATGTTGTGG3710.10316932839826141No Hit
CTGCATCTGCCGTTCCGGCCGACCACGGGGCGCACCTCTCTTTACGCGGT3690.10261315951201742No Hit
CCATGTAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAA3680.10233507506889543No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTCACATTCATTTACAGG3650.10150082173952943No Hit
GCAGTAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGAAC3640.10122273729640742No Hit
GTGTAGTTTCTCTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAA3600.10011039952391944No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)