FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488244

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488244
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences368856
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC19160.5194439022274275No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN16930.4589867048387447No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA16350.44326241134751776No Hit
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA11320.3068948315873945No Hit
CTAATCTCCTCCCCCAACTCCTCCCAGTCCTTAAACAAACAGTCTTTGAA10540.285748367926779No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACATAGCGCC10050.27246405101177695No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTATAGAGAGTCCAA9560.2591797340967749No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATGCGTTGACAGACT9210.24969093630034483No Hit
ACATTATAGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAAT9070.24589541718177282No Hit
ATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTCACGGTGGTCTCCATGC8210.22258008545340185No Hit
GGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTCTGTGTAAACAATACC7960.21580237274166614No Hit
TCTAGATACCGCCTCAGCTCTGTATCGGGAAGCCTTAGAGTCTCCTGAGC7910.21444683019931896No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC7890.21390461318238013No Hit
AGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTCTATGA7650.2073980089791138No Hit
AGCTTGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTCTATGA7540.20441581538595005No Hit
CCTCAAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA7470.20251805582666405No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTC7390.20034918775890861No Hit
CTATAATGTCAACGACCGACCTTGAGGCATACTTCAAAGACTGTTTGTTT7260.19682477714880603No Hit
GAATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGA7060.19140260697941744No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT6930.18787819636931485No Hit
GTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGC6590.17866050708135425No Hit
GGTTCAAGATCCTGGAATTAGAGGACAAACGGGCAACATACCTTGATAGT6530.17703385603053765No Hit
TTATTTACACACTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAA6450.17486498796278224No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCCGTACG6370.17269611989502678No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCACATAACTAAAAGCCAAA6360.17242501138655736No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC6240.1691717092849242No Hit
CATTATAGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAATT6200.1680872752510465No Hit
GGATTGGGCCTCAGCCCGTTTCTCATGGCTCAGTTTACTAGTGCCATTTG6170.1672739497256382No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG6090.16510508165788276No Hit
CTCTATGACCGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA6090.16510508165788276No Hit
CTTCTAGATACCGCCTCAGCTCTGTATCGGGAAGCCTTAGAGTCTCCTGA5990.16239399657318845No Hit
GTGTAAACAATACCTGAACCTTTACCCCGTTGCCCGGCAACGGCCAGGTC5950.16130956253931072No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC5780.15670071789533044No Hit
TCTTCAACCACCAGCACGGGACCATGCAGAACCTGCACGACTCCTGCTCA5730.15534517535298326No Hit
GTGTAAATGTATATTAGGAAAAGAGGGTGTTTTCCAATGAGGGTTAAAGA5710.1548029583360444No Hit
CCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG5640.1529051987767584No Hit
GCGTTGACAGACTTTCCAATCAATAGGCCTATTAACAGGAAGTTTTCTAA5510.14938078816665584No Hit
ATACCACATCATCCACATAACTAAAAGCCAAACAGTGGGGGAAAGCCCTA5480.14856746264124754No Hit
ATATAAGAGAGAAACAACACATAGCGCCTCATTCTGTGGGTCACCATATT5450.14775413711583923No Hit
CTTCTACTGTTCCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCT5410.1466697030819615No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAAT5380.14585637755655323No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCTTTGTATGCATGTATT5370.1455852690480838No Hit
CATTTAAACCCTAACAAAACAAAAAGGTGGGGTTACTCTTTACATTTTAT5260.14260307545492007No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCTATAATGTCAACGACCGACCT5250.14233196694645064No Hit
ATTATAGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAATTG5200.1409764244041035No Hit
GGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT5170.1401630988786952No Hit
CTCTATAATGTCAACGACCGACCTTGAGGCATACTTCAAAGACTGTTTGT5130.13907866484481748No Hit
GTAGAAGAGTAAAGCCCCGTAAAGTTTCCCACCTTATGAGTCCAAGGAAT5090.13799423081093976No Hit
GTATCGGGAAGCCTTAGAGTCTCCTGAGCATTGTTCACCTCACCATACTG4870.13202984362461231No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA4810.13040319257379573No Hit
GTATGATGTGATCCTGTGGCAATGACCCATAACATCCAATGACATAGCCC4640.12579434792981542No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT4610.12498102240440713No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCCGTG4580.12416769687899885No Hit
GCCTTAGAGTCTCCTGAGCATTGTTCACCTCACCATACTGCACTCAGGCA4530.12281215433665169No Hit
GCACGGGACCATGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATG4530.12281215433665169No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGAACAACAGAGT4500.12199882881124341No Hit
ATGTGATCCTGTGGCAATGACCCATAACATCCAATGACATAGCCCATAAA4450.12064328626889627No Hit
CATTAGTGTTAACATAACTGACTACTAGGTCCCTGGATACTGGATCTTCC4430.1201010692519574No Hit
CCTCTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGC4320.11711887565879367No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC4300.11657665864185482No Hit
CTAGTAAACTGAGCCATGAGAAACGGGCTGAGGCCCAATCCCATAGGAAT4280.11603444162491595No Hit
GTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAAC4250.11522111609950766No Hit
GTATGGATCGGCAGAGGAGCCAGAAAGGTTCCACGCATGCGCTGATGGCC4230.11467889908256881No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG4210.11413668206562994No Hit
GTCATAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC4140.11223892250634393No Hit
TTATATAAGAGAGAAACAACACATAGCGCCTCATTCTGTGGGTCACCATA4120.11169670548940508No Hit
CAATAGGCCTATTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGT4110.11142559698093565No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACC4090.1108833799639968No Hit
GGGTTTACTGTTCCTGAACTGGAGCCACCAGCAGGGAAATACAGGCCTCT4070.11034116294705792No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCTTCAACCACC4060.1100700544385885No Hit
GTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGATAA3990.10817229487930248No Hit
GTTATGTTAACACTAATGTGGGCCTAAAGTTTAGACAACTATTGTGGTTT3910.10600342681154706No Hit
GTATGCATGTATTCAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACA3880.10519010128613876No Hit
GTGTAAGTAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT3850.10437677576073047No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGA3850.10437677576073047No Hit
GTTAGTATTCCTTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTACTC3830.10383455874379162No Hit
CATGCATACAAAGGCATTAAAGCAGGATAACCACATTGTGTAAAAGGGGC3780.10247901620144446No Hit
GTACCAAACCTTCGGACGGAAATTGCACCTGTATTCCCATCCCATCATCC3760.10193679918450561No Hit
CAGTAAACCCTGTTCCGACTACTGTCTCTCACATATCGTCAATCTTCTCG3730.10112347365909731No Hit
ATGTATATTTGCGGGAGAGAACAACAGAGTTATCTGTCCCGAGAATGTTT3730.10112347365909731No Hit
GTTTAAATGTATACCCAAAGACACAAGAAAATTGGTAACAGCGGTAAAAA3710.10058125664215846No Hit
TAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATGCG3690.10003903962521961No Hit
ATGTGGTTATCCTGCTTTAATGCCTTTGTATGCATGTATTCAATCTAAGC3690.10003903962521961No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)