FastQCFastQC Report
Fri 1 Jul 2022
EGAF00002488247

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488247
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences388834
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG29640.7622790188100835No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT28850.741961865474727No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT25750.6622363270701637No Hit
CATTTAAACCCTAACAAAACAAAAAGGTGGGGTTACTCTTTACATTTTAT25440.6542637732297072No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAAT20990.5398190487457373No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC17960.4618937644341801No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGT15790.4060858875509858No Hit
CTCTTTACATTTTATGGGCTATGTCATTGGATGTTATGGGTCATTGCCAC15570.40042794611582316No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA14420.37085234315929166No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCTTTGTATGCATGTATT13890.3572218478836727No Hit
GTCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCTTCAACCACC13750.3536213396976602No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA13510.34744903995021015No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC13500.34719186079406633No Hit
TACCTTGACAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG12660.3255888116779911No Hit
TAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACG12630.32481727420955986No Hit
GAATACATGCATACAAAGGCATTAAAGCAGGATAACCACATTGTGTAAAA12050.3099008831532222No Hit
CATGCATACAAAGGCATTAAAGCAGGATAACCACATTGTGTAAAAGGGGC12030.3093865248409347No Hit
ATGTCATTGGATGTTATGGGTCATTGCCACAAGATCACATCATACAGAAA12030.3093865248409347No Hit
GCACGGGACCATGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATG11730.3016711501566221No Hit
GTATGATGTGATCTTGTGGCAATGACCCATAACATCCAATGACATAGCCC11620.29884217943904084No Hit
GTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC11620.29884217943904084No Hit
GCTTAGATTGAATACATGCATACAAAGGCATTAAAGCAGGATAACCACAT11430.29395577547230956No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACC11290.290355267286297No Hit
GAACAAATGGCACTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCAC11260.28958372981786573No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTG11230.2888121923494345No Hit
GCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTCTTTGG10930.2810968176651219No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAG10880.2798109218844031No Hit
GTCCCGTGCTGGTGGTTGAAGATCCTGGAATTAGAGGACAAACGGGCAAC10640.273638622136953No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT10590.2723527263562343No Hit
TGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCTT10510.2702952931070843No Hit
CCTCTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGC10390.2672091432333592No Hit
GTTATGGGTCATTGCCACAAGATCACATCATACAGAAAATCAAAGAATGT10370.26669478492107174No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA10180.26180838095434045No Hit
CAGTAAACCCTGTTCCGACTACTGTCTCTCACATATCGTCAATCTTCTCG10100.2597509477051904No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA10080.2592365893929029No Hit
GTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGTATGATGTGAT9560.24586327327342775No Hit
GTCTTTGGGTATACATTTAAACCCTAACAAAACAAAAAGGTGGGGTTACT9490.24406301918042145No Hit
TTGACAGACTTTCCAATCAATAGGCCTGTTAACAGGAAGTTTTCTAAAAC9390.24149122761898392No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCCGTG9250.23789071943297138No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT9160.23557610702767762No Hit
GATTGAATACATGCATACAAAGGCATTAAAGCAGGATAACCACATTGTGT9150.23531892787153388No Hit
CATTAAAGCAGGATAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAA8790.22606047825035877No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGAAGAGGA8660.22271714922048996No Hit
GGGTTACTCTTTACATTTTATGGGCTATGTCATTGGATGTTATGGGTCAT8590.22091689512748372No Hit
ATGTAAAGAGTAACCCCACCTTTTTGTTTTGTTAGGGTTTAAATGTATAC8560.22014535765905244No Hit
CCTGTATTCCCATCCCATCATCCTGGGCTTTCGGAAAATTCCTATGGGAG8480.2180879244099024No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA8410.21628767031689616No Hit
AGATCACATCATACAGAAAATCAAAGAATGTTTTAGAAAACTTCCTGTTA8340.21448741622388987No Hit
GGCCTATTGATTGGAAAGTCTGTCAACGTATTGTGGGTCTTTTGGGTTTT7990.20548614575885854No Hit
ATGTGATCTTGTGGCAATGACCCATAACATCCAATGACATAGCCCATAAA7860.20214281672898976No Hit
GTTCAGGAACAGTAAACCCTGTTCCGACTACTGTCTCTCACATATCGTCA7800.20059974179212725No Hit
ACATCATACAGAAAATCAAAGAATGTTTTAGAAAACTTCCTGTTAACAGG7730.19879948769912095No Hit
CTTTCCAATCAATAGGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTG7650.19674205444997092No Hit
GATACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCC7420.19082693385866464No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC7360.1892838589218021No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG7330.18851232145337085No Hit
ACCCATAACATCCAATGACATAGCCCATAAAATGTAAAGAGTAACCCCAC7280.1872264256726521No Hit
GGCTATGTCATTGGATGTTATGGGTCATTGCCACAAGATCACATCATACA7230.18594052989193333No Hit
ACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTT7230.18594052989193333No Hit
GAACATGGAGAACATCACATCAGGATTCCTAGGACCCCTGCTCGTGTTAC7190.1849118132673583No Hit
GGGTATACATTTAAACCCTAACAAAACAAAAAGGTGGGGTTACTCTTTAC7150.1838830966427833No Hit
CCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAA7150.1838830966427833No Hit
GAGTAACCCCACCTTTTTGTTTTGTTAGGGTTTAAATGTATACCCAAAGA7050.18131130508134577No Hit
GGTATACATTTAAACCCTAACAAAACAAAAAGGTGGGGTTACTCTTTACA7020.18053976761291451No Hit
ATGTGGTTATCCTGCTTTAATGCCTTTGTATGCATGTATTCAATCTAAGC7000.180025409300627No Hit
CTTTACATTTTATGGGCTATGTCATTGGATGTTATGGGTCATTGCCACAA6920.177967976051477No Hit
ATACGTTGACAGACTTTCCAATCAATAGGCCTGTTAACAGGAAGTTTTCT6630.17050978052330815No Hit
GTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGT6610.16999542221102062No Hit
CATTGGATGTTATGGGTCATTGCCACAAGATCACATCATACAGAAAATCA6610.16999542221102062No Hit
CTTCTGGACTGTCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATC6550.16845234727415812No Hit
GTACCAAACCTTCGGACGGAAATTGCACCTGTATTCCCATCCCATCATCC6480.16665209318115184No Hit
GACATAGCCCATAAAATGTAAAGAGTAACCCCACCTTTTTGTTTTGTTAG6450.1658805557127206No Hit
GTCTCTCACATATCGTCAATCTTCTCGAGGATTGGGGACCCTGCGCTGAA6370.16382312246357056No Hit
ACCGTGTGTCTTGGCCAAAATTCGCAGTCCCCAACCTCCAATCACTCACC6340.1630515849951393No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN6340.1630515849951393No Hit
GTATGCATGTATTCAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACA6240.1604797934337018No Hit
GGATGTTATGGGTCATTGCCACAAGATCACATCATACAGAAAATCAAAGA6210.15970825596527052No Hit
GAACTACCGTGTGTCTTGGCCAAAATTCGCAGTCCCCAACCTCCAATCAC6180.15893671849683927No Hit
TTTAAACCCTAACAAAACAAAAAGGTGGGGTTACTCTTTACATTTTATGG6020.15482185199853923No Hit
ATCCTGGGCTTTCGGAAAATTCCTATGGGAGTGGGCCTCAGCCCGTTTCT6010.15456467284239547No Hit
GAATACAGGTGCAATTTCCGTCCGAAGGTTTGGTACAGCAACAGGAGGGA5930.15250723959324544No Hit
GCCCATAAAATGTAAAGAGTAACCCCACCTTTTTGTTTTGTTAGGGTTTA5870.15096416465638293No Hit
GACCTGGCCGTTGCCGGGCAACGGGGTAAAGGTTCAGGTATTGTTTACAC5860.15070698550023917No Hit
CTCACATATCGTCAATCTTCTCGAGGATTGGGGACCCTGCGCTGAACATG5860.15070698550023917No Hit
GGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCC5750.14787801478265789No Hit
GTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACCGC5730.1473636564703704No Hit
GCTTTAATGCCTTTGTATGCATGTATTCAATCTAAGCAGGCTTTCACTTT5730.1473636564703704No Hit
ACCCAAAAGACCCACAATACGTTGACAGACTTTCCAATCAATAGGCCTGT5720.14710647731422663No Hit
CCTTTTTACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA5690.14633493984579538No Hit
GCCCAGGATGATGGGATGGGAATACAGGTGCAATTTCCGTCCGAAGGTTT5650.14530622322122036No Hit
GTCATTGGATGTTATGGGTCATTGCCACAAGATCACATCATACAGAAAAT5650.14530622322122036No Hit
CTCCCATAGGAATTTTCCGAAAGCCCAGGATGATGGGATGGGAATACAGG5570.14324878997207033No Hit
TTATGGGTCATTGCCACAAGATCACATCATACAGAAAATCAAAGAATGTT5570.14324878997207033No Hit
CAATGACATAGCCCATAAAATGTAAAGAGTAACCCCACCTTTTTGTTTTG5540.14247725250363907No Hit
ACTCTTTACATTTTATGGGCTATGTCATTGGATGTTATGGGTCATTGCCA5530.14222007334749534No Hit
GGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTG5490.14119135672292032No Hit
GACCCATAACATCCAATGACATAGCCCATAAAATGTAAAGAGTAACCCCA5480.14093417756677656No Hit
ACAATACGTTGACAGACTTTCCAATCAATAGGCCTGTTAACAGGAAGTTT5400.13887674431762653No Hit
ACCCTGTTCCGACTACTGTCTCTCACATATCGTCAATCTTCTCGAGGATT5400.13887674431762653No Hit
GCCCATGACCAAGCCCCAGCCAGTGGGGGTTGCGTCAGCAAACACTTGGC5360.13784802769305152No Hit
GGATGATGGGATGGGAATACAGGTGCAATTTCCGTCCGAAGGTTTGGTAC5340.13733366938076402No Hit
GTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTA5330.13707649022462026No Hit
CCTGTTAACAGGCCTATTGATTGGAAAGTCTGTCAACGTATTGTGGGTCT5310.13656213191233277No Hit
GTGAAAGCCTGCTTAGATTGAATACATGCATACAAAGGCATTAAAGCAGG5260.135276236131614No Hit
GGTTATCGCTGGATGTGTCTGCGGCGTTTTATCATCTTCCTCTTCATCCT5250.13501905697547023No Hit
GGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTCTGTGTAAACAATACC5250.13501905697547023No Hit
GTCCCCAACCTCCAATCACTCACCAACCTCCTGTCCTCCAACTTGTCCTG5230.13450469866318274No Hit
ATAACATCCAATGACATAGCCCATAAAATGTAAAGAGTAACCCCACCTTT5200.13373316119475148No Hit
CTCTCACATATCGTCAATCTTCTCGAGGATTGGGGACCCTGCGCTGAACA5190.13347598203860772No Hit
CAATACCGCAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGG5180.13321880288246396No Hit
CCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAAGGGACTCAAGATG5160.1327044445701765No Hit
ACAAAAAGGTGGGGTTACTCTTTACATTTTATGGGCTATGTCATTGGATG5130.13193290710174524No Hit
GTATACATTTAAACCCTAACAAAACAAAAAGGTGGGGTTACTCTTTACAT5100.13116136963331396No Hit
GGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAA5070.1303898321648827No Hit
ATGCTGTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAG5060.13013265300873897No Hit
ACGTTGACAGACTTTCCAATCAATAGGCCTGTTAACAGGAAGTTTTCTAA4990.1283323989157327No Hit
TTTTATGGGCTATGTCATTGGATGTTATGGGTCATTGCCACAAGATCACA4980.12807521975958894No Hit
CAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCTTAGATTGAA4950.12730368229115768No Hit
CAATACGTTGACAGACTTTCCAATCAATAGGCCTGTTAACAGGAAGTTTT4900.1260177865104389No Hit
CCCATAGGAATTTTCCGAAAGCCCAGGATGATGGGATGGGAATACAGGTG4900.1260177865104389No Hit
TCACAATACCGCAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTA4860.12498906988586389No Hit
AGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAATTT4830.12421753241743264No Hit
ATTTCCGTCCGAAGGTTTGGTACAGCAACAGGAGGGATACATAGAGGTTC4830.12421753241743264No Hit
GGTTTGGTACAGCAACAGGAGGGATACATAGAGGTTCCTTGAGCAGGAGT4820.12396035326128889No Hit
GCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAGCCAGGAGAAA4820.12396035326128889No Hit
CACTTGGCACAGACCTGGCCGTTGCCGGGCAACGGGGTAAAGGTTCAGGT4820.12396035326128889No Hit
GTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGA4780.12293163663671387No Hit
CCCCAACCTCCAATCACTCACCAACCTCCTGTCCTCCAACTTGTCCTGGT4770.12267445748057011No Hit
CTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTG4670.12010266591913259No Hit
CATCATACAGAAAATCAAAGAATGTTTTAGAAAACTTCCTGTTAACAGGC4630.11907394929455757No Hit
CATCCAATGACATAGCCCATAAAATGTAAAGAGTAACCCCACCTTTTTGT4620.11881677013841382No Hit
GGATGAAGAGGAAGATGATAAAACGCCGCAGACACATCCAGCGATAACCA4580.11778805351383881No Hit
ATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACCTG4580.11778805351383881No Hit
CATGTATTCAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC4530.11650215773312005No Hit
GTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAACCCTAACAAAAC4490.11547344110854503No Hit
GGACCATGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATGTATCC4480.11521626195240128No Hit
CCTTGACAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGAT4440.11418754532782627No Hit
GCCCCAGCCAGTGGGGGTTGCGTCAGCAAACACTTGGCACAGACCTGGCC4430.11393036617168252No Hit
TTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCT4430.11393036617168252No Hit
CCTCCAACTTGTCCTGGTTATCGCTGGATGTGTCTGCGGCGTTTTATCAT4400.11315882870325125No Hit
CAGTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTG4370.11238729123482No Hit
CAATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCC4340.11161575376638874No Hit
GTCTGTCAACGTATTGTGGGTCTTTTGGGTTTTGCTGCCCCTTTTACACA4310.11084421629795747No Hit
GTTAACAGGCCTATTGATTGGAAAGTCTGTCAACGTATTGTGGGTCTTTT4310.11084421629795747No Hit
CAATGTGGTTATCCTGCTTTAATGCCTTTGTATGCATGTATTCAATCTAA4290.11032985798566997No Hit
ATGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATGTATCCCTCCT4290.11032985798566997No Hit
CCACTGAACAAATGGCACTAGTAAACTGAGCCAGGAGAAACGGGCTGAGG4280.11007267882952622No Hit
GTATTCCCATCCCATCATCCTGGGCTTTCGGAAAATTCCTATGGGAGTGG4220.1085296038926637No Hit
TTTTACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAACC4190.10775806642423244No Hit
GTTTTATCATCTTCCTCTTCATCCTGCTGCTATGCCTCATCTTCTTGTTG4180.1075008872680887No Hit
CGGTAGTTCCCCCTAGAAAATTGAGAGAAGTCCACCACGAGTCTAGACTC4180.1075008872680887No Hit
GCAACAGGAGGGATACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTC4170.10724370811194493No Hit
TCCAATGACATAGCCCATAAAATGTAAAGAGTAACCCCACCTTTTTGTTT4170.10724370811194493No Hit
GGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGTATGAT4160.10698652895580119No Hit
TGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATGTATCCCTCCTG4150.10672934979965742No Hit
CTCACCAACCTCCTGTCCTCCAACTTGTCCTGGTTATCGCTGGATGTGTC4150.10672934979965742No Hit
CCTCACAATACCGCAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTC4150.10672934979965742No Hit
ATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAAGGGACT4130.10621499148736992No Hit
CCATCATCCTGGGCTTTCGGAAAATTCCTATGGGAGTGGGCCTCAGCCCG4110.10570063317508242No Hit
GCTATGCCTCATCTTCTTGTTGGTTCTTCTGGACTGTCAAGGTATGTTGC4110.10570063317508242No Hit
TTTATGGGCTATGTCATTGGATGTTATGGGTCATTGCCACAAGATCACAT4080.10492909570665115No Hit
ATACCGCAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGG4070.10467191655050741No Hit
CCCATAAAATGTAAAGAGTAACCCCACCTTTTTGTTTTGTTAGGGTTTAA4060.10441473739436365No Hit
GTGGTTATCCTGCTTTAATGCCTTTGTATGCATGTATTCAATCTAAGCAG4020.10338602076978864No Hit
CAGTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTG3990.1026144833013574No Hit
ATTGGGGACCCTGCGCTGAACATGGAGAACATCACATCAGGATTCCTAGG3990.1026144833013574No Hit
GTTCCGACTACTGTCTCTCACATATCGTCAATCTTCTCGAGGATTGGGGA3980.10235730414521364No Hit
TTACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAACCCT3970.1021001249890699No Hit
CTGATGGCCCATGACCAAGCCCCAGCCAGTGGGGGTTGCGTCAGCAAACA3910.10055705005220737No Hit
GCAGAGGAGCCAGAAAGGTTCCACGCATGCGCTGATGGCCCATGACCAAG3890.10004269173991986No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)