FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002488248

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488248
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences520276
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN46850.9004835894794302No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT34410.6613797292206445No Hit
CTTTACATTTTATGGGCTATGTCATTGGATGTTATGGGTCATTGCCACAA29420.5654690971715013No Hit
GTTTCCTAAAACATTCTTTGATTTTCTGTATGATGTGATCTTGTGGCAAT26040.5005035788696769No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC20580.395559280074422No Hit
CATTTAAACCCTAACAAAACAAAAAGGTGGGGTTACTCTTTACATTTTAT16900.3248275915091221No Hit
GTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC16890.3246353858336729No Hit
ATACGCTGACAGACTTTCCAATCAATAGGCCTGTTAACAGGAAGTTTTCT16820.3232899461055286No Hit
ACTCCTGCTCAAGGAACCTCTATGTATCCCTCCTGTTGCTGTACCAAACC16170.3107965772013316No Hit
GCCTAGCAACGGGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTG16120.30983554882408565No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG14180.27254764778694385No Hit
GAACCACCGTGTGTCTTGGCCAAAATTCGCAGTCCCCAACCTCCAATCAC13990.2688957399534093No Hit
CGGTAGTTCCCCCTAGAAAATTGAGAGAAGTCCACCACGAGTCTAGACTC13830.2658204491462224No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA13460.2587088391546026No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGT12840.2467920872767531No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAAT12840.2467920872767531No Hit
CTCTTTACATTTTATGGGCTATGTCATTGGATGTTATGGGTCATTGCCAC12720.24448561917136288No Hit
CCCCACCTTTTTGTTTTGTTAGGGTTTAAATGTATACCCAAAGACAAAAG12550.24121812268872675No Hit
GTCAACGTATTGTGGGTCTTTTGGGTTTTGCTGCCCCTTTTACACAATGT12220.23487533539890368No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT12220.23487533539890368No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGCTGACAGACT12050.23160783891626752No Hit
CTTTAAATTTCATGGGCTATGTCATTGGATGTTATGGGTCATTGCCACAA11830.22737931405638545No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA11640.22372740622285092No Hit
GCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTCTTTGG11370.21853785298572298No Hit
AAGATGGGGTTACTCTTTAAATTTCATGGGCTATGTCATTGGATGTTATG11360.21834564731027378No Hit
CATGCATACAAAGGCATTAAAGCAGGATAACCACATTGTGTAAAAGGGGC10870.20892756921326375No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC10350.19893287408990612No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCTTTGTATGCATGTATT10130.19470434923002405No Hit
ATGTCATTGGATGTTATGGGTCATTGCCACAAGATCACATCATACAGAAA10040.19297449815098142No Hit
GTTATGGGTCATTGCCACAAGATCACATCATACAGAAAATCAAAGAATGT9650.18547847680846322No Hit
TACGCTGACAGACTTTCCAATCAATAGGCCTGTTAACAGGAAGTTTTCTA9380.18028892357133522No Hit
GAATACATGCATACAAAGGCATTAAAGCAGGATAACCACATTGTGTAAAA9320.17913568951864012No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA9300.17875127816774175No Hit
CCTGTATTCCCATCCCATCATCCTGGGCTTTCGGAAAATTCCTATGGGAG9280.17836686681684338No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT9270.17817466114139419No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAG9020.17336951925516456No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA8970.17240849087791865No Hit
ACAACACGTTGACAGACTTTCCAATCAATAGGCCTGTTAACAGGAAGTTT8870.1704864341234268No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTG8530.16395144115815452No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACC8400.16145276737731512No Hit
GAACTACCGTGTGTCTTGGCCAAAATTCGCAGTCCCCAACCTCCAATCAC8290.1593385049473741No Hit
TACATGCATACAAAGGCATTAAAGCAGGATAACCACATTGTGTAAAAGGG8260.15876188792102655No Hit
TGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCTT8230.158185270894679No Hit
GAAGATGAGGCATAGCAGCAGGATGAAGAGGAAGATGATAAAACGCCGCA8190.15741644819288225No Hit
CCTCTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGC8100.1556865971138396No Hit
GTATGATGTGATCTTGTGGCAATGACCCATAACATCCAATGACATAGCCC7980.15338012900844936No Hit
TAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACG7780.14953601549946566No Hit
GTTTTCTAAAACATTCTTTGATTTTCTGTATGATGTGATCTTGTGGCAAT7700.1479983700958722No Hit
ATGTAAAGAGTAACCCCACCTTTTTGTTTTGTTAGGGTTTAAATGTATAC7640.1468451360431771No Hit
GAACAAATGGCACTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCAC7580.14569190199048196No Hit
AATTTAAAGAGTAACCCCATCTTTTAGTTTTGTTAGGGTTTAAATGTATA7540.14492307928868525No Hit
AGACTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT7520.14453866793778689No Hit
GTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGTATGATGTGAT7300.1403101430779048No Hit
GAGCAGTAGTCGTGCAGGTTCTGCATGGTCCCGTGCTGGTGGTTGAAGAT7060.1356972068671244No Hit
AAACTAAAAGATGGGGTTACTCTTTAAATTTCATGGGCTATGTCATTGGA7060.1356972068671244No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG7010.13473617848987846No Hit
AATTTAAAGAGTAACCCCACCTTTTTGTTTTGTTAGGGTTTAAATGTATA7000.13454397281442926No Hit
GTCTTTGGGTATACATTTAAACCCTAACAAAACAAAAAGGTGGGGTTACT6950.13358294443718333No Hit
GGCTATGTCATTGGATGTTATGGGTCATTGCCACAAGATCACATCATACA6860.1318530933581407No Hit
GAATACAGGTGCAATTTCCGTCCGAAGGTTTGGTACAGCAACAGGAGGGA6840.13146868200724232No Hit
GTACCAAACCTTCGGACGGAAATTGCACCTGTATTCCCATCCCATCATCC6720.1291622139018521No Hit
GACCTGGCCGTTGCCGGGCAACGGGGTAAAGGTTCAGGTATTGTTTACAC6690.12858559687550453No Hit
AATGTAAAGAGTAACCCCACCTTTTTGTTTTGTTAGGGTTTAAATGTATA6630.12743236282280943No Hit
GCACGGGACCATGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATG6630.12743236282280943No Hit
CCTTTTTACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA6590.1266635401210127No Hit
TTTTTGTTTTGTTAGGGTTTAAATGTATACCCAAAGACAAAAGAAAATTG6530.12551030606831756No Hit
ATTTCATGGGCTATGTCATTGGATGTTATGGGTCATTGCCACAAGATCAC6470.12435707201562249No Hit
AGATCACATCATACAGAAAATCAAAGAATGTTTTAGAAAACTTCCTGTTA6460.1241648663401733No Hit
CATTTAAACCCTAACAAAACTAAAAGATGGGGTTACTCTTTAAATTTCAT6420.12339604363837656No Hit
GAGTAACCCCACCTTTTTGTTTTGTTAGGGTTTAAATGTATACCCAAAGA6310.12128178120843552No Hit
ACAATACGCTGACAGACTTTCCAATCAATAGGCCTGTTAACAGGAAGTTT6250.12012854715574041No Hit
GGATGATGGGATGGGAATACAGGTGCAATTTCCGTCCGAAGGTTTGGTAC6200.11916751877849448No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA6180.11878310742759611No Hit
ATACGCTGACAGACTTTCCAATCAATAGGTCTGTTAACAGGAAGTTTCCT6180.11878310742759611No Hit
ATCCTGGGCTTTCGGAAAATTCCTATGGGAGTGGGCCTCAGCCCGTTTCT6170.11859090175214693No Hit
CCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAA6140.11801428472579939No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCCGTG6140.11801428472579939No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGAAGAGGA6130.1178220790503502No Hit
CAGTAAACCCTGTTCCGACTACTGTCTCTCACATATCGTCAATCTTCTCG6060.11647663932220591No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT6060.11647663932220591No Hit
CATTAAAGCAGGATAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAA6030.11590002229585836No Hit
GCCGGGCAACGGGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTG5960.11455458256771406No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACCACCGTG5940.11417017121681569No Hit
GCCCAGGATGATGGGATGGGAATACAGGTGCAATTTCCGTCCGAAGGTTT5860.11263252581322221No Hit
GCGCTGAACATGGAGAACATCACATCAGGATTCCTAGGACCCCTGCTCGT5850.11244032013777303No Hit
GGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTG5680.10917282365513689No Hit
GTCATTGGATGTTATGGGTCATTGCCACAAGATCACATCATACAGAAAAT5600.10763517825154341No Hit
CATTGGATGTTATGGGTCATTGCCACAAGATCACATCATACAGAAAATCA5590.10744297257609423No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC5560.10686635554974666No Hit
ACATCATACAGAAAATCAAAGAATGTTTTAGAAAACTTCCTGTTAACAGG5540.1064819441988483No Hit
GTCCCGTGCTGGTGGTTGAAGATCCTGGAATTAGAGGACAAACGGGCAAC5510.10590532717250074No Hit
CAATAGGTCTGTTAACAGGAAGTTTCCTAAAACATTCTTTGATTTTCTGT5480.10532871014615319No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG5440.10455988744435647No Hit
GGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCC5410.10398327041800891No Hit
GTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGT5400.10379106474255972No Hit
GGACAAACGGGCAACATACCTTGATAGTCCAGAAGAACCAACAAGAAGAT5380.10340665339166136No Hit
GTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAACCCTAACAAAAC5370.10321444771621217No Hit
GCTTTAATGCCTTTGTATGCATGTATTCAATCTAAGCAGGCTTTCACTTT5330.10244562501441543No Hit
GCTTAGATTGAATACATGCATACAAAGGCATTAAAGCAGGATAACCACAT5290.10167680231261869No Hit
CCACTGAACAAATGGCACTAGTAAACTGAGCCAGGAGAAACGGGCTGAGG5250.10090797961082194No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)