FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002488249

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488249
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences520276
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN46770.8989459440758367No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT34800.6688757505631626No Hit
CTTTACATTTTATGGGCTATGTCATTGGATGTTATGGGTCATTGCCACAA28360.5450952955738877No Hit
GTTTCCTAAAACATTCTTTGATTTTCTGTATGATGTGATCTTGTGGCAAT26830.5156878272301624No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC19820.38095164874028403No Hit
GTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC18230.35039094634386364No Hit
CATTTAAACCCTAACAAAACAAAAAGGTGGGGTTACTCTTTACATTTTAT16190.31118098855223No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG16140.31021996017498404No Hit
GAACCACCGTGTGTCTTGGCCAAAATTCGCAGTCCCCAACCTCCAATCAC15750.30272393883246584No Hit
GTCAACGTATTGTGGGTCTTTTGGGTTTTGCTGCCCCTTTTACACAATGT15720.3021473218061183No Hit
ATACGCTGACAGACTTTCCAATCAATAGGCCTGTTAACAGGAAGTTTTCT15450.2969577685689903No Hit
GCCTAGCAACGGGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTG14440.27754499534862265No Hit
ACTCCTGCTCAAGGAACCTCTATGTATCCCTCCTGTTGCTGTACCAAACC14090.2708177967079012No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA13860.2663970661725699No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGCTGACAGACT13480.2590932505055009No Hit
CCCCACCTTTTTGTTTTGTTAGGGTTTAAATGTATACCCAAAGACAAAAG13260.25486472564561885No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAAT13210.2539036972683729No Hit
CGGTAGTTCCCCCTAGAAAATTGAGAGAAGTCCACCACGAGTCTAGACTC13180.2533270802420254No Hit
CTCTTTACATTTTATGGGCTATGTCATTGGATGTTATGGGTCATTGCCAC12990.2496751724084909No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGT12140.23333768999531018No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA11950.22968578216177568No Hit
GCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTCTTTGG11370.21853785298572298No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT10870.20892756921326375No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCTTTGTATGCATGTATT10840.20835095218691618No Hit
CTTTAAATTTCATGGGCTATGTCATTGGATGTTATGGGTCATTGCCACAA10830.208158746511467No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC10580.20335360462523738No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT10330.19854846273900775No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACC9760.18759273923840422No Hit
ATGTCATTGGATGTTATGGGTCATTGCCACAAGATCACATCATACAGAAA9620.18490185978211565No Hit
GTTATGGGTCATTGCCACAAGATCACATCATACAGAAAATCAAAGAATGT9590.1843252427557681No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA9580.18413303708031892No Hit
CATGCATACAAAGGCATTAAAGCAGGATAACCACATTGTGTAAAAGGGGC9570.18394083140486972No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAG9510.18278759735217462No Hit
GAACTACCGTGTGTCTTGGCCAAAATTCGCAGTCCCCAACCTCCAATCAC9450.1816343632994795No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTG9300.17875127816774175No Hit
TACGCTGACAGACTTTCCAATCAATAGGCCTGTTAACAGGAAGTTTTCTA9280.17836686681684338No Hit
GTATGATGTGATCTTGTGGCAATGACCCATAACATCCAATGACATAGCCC8950.17202407952702028No Hit
AAGATGGGGTTACTCTTTAAATTTCATGGGCTATGTCATTGGATGTTATG8750.1681799660180366No Hit
GAATACATGCATACAAAGGCATTAAAGCAGGATAACCACATTGTGTAAAA8670.1666423206144431No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA8520.16375923548270532No Hit
CCTGTATTCCCATCCCATCATCCTGGGCTTTCGGAAAATTCCTATGGGAG8320.15991512197372162No Hit
GAACAAATGGCACTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCAC8130.15626321414018712No Hit
TAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACG8060.15491777441204282No Hit
TGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCTT7990.15357233468389853No Hit
CCTCTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGC7870.15126586657850832No Hit
GTTTTCTAAAACATTCTTTGATTTTCTGTATGATGTGATCTTGTGGCAAT7850.15088145522760996No Hit
AGACTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT7390.14203999415694746No Hit
GTCTTTGGGTATACATTTAAACCCTAACAAAACAAAAAGGTGGGGTTACT7240.1391569090252097No Hit
ACAACACGTTGACAGACTTTCCAATCAATAGGCCTGTTAACAGGAAGTTT7240.1391569090252097No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT7210.13858029199886215No Hit
GTACCAAACCTTCGGACGGAAATTGCACCTGTATTCCCATCCCATCATCC7200.13838808632341296No Hit
GTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGTATGATGTGAT7080.13608161821802275No Hit
ATACGCTGACAGACTTTCCAATCAATAGGTCTGTTAACAGGAAGTTTCCT6950.13358294443718333No Hit
TACATGCATACAAAGGCATTAAAGCAGGATAACCACATTGTGTAAAAGGG6670.12820118552460616No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC6630.12743236282280943No Hit
GAATACAGGTGCAATTTCCGTCCGAAGGTTTGGTACAGCAACAGGAGGGA6610.12704795147191106No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACCACCGTG6550.12589471741921596No Hit
AATTTAAAGAGTAACCCCATCTTTTAGTTTTGTTAGGGTTTAAATGTATA6520.12531810039286842No Hit
ATTTCATGGGCTATGTCATTGGATGTTATGGGTCATTGCCACAAGATCAC6500.12493368904197004No Hit
AATTTAAAGAGTAACCCCACCTTTTTGTTTTGTTAGGGTTTAAATGTATA6470.12435707201562249No Hit
GGCTATGTCATTGGATGTTATGGGTCATTGCCACAAGATCACATCATACA6460.1241648663401733No Hit
CCTTTTTACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA6380.12262722093657982No Hit
GACCTGGCCGTTGCCGGGCAACGGGGTAAAGGTTCAGGTATTGTTTACAC6370.12243501526113063No Hit
GTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGT6330.12166619255933389No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA6310.12128178120843552No Hit
GAAGATGAGGCATAGCAGCAGGATGAAGAGGAAGATGATAAAACGCCGCA6290.12089736985753716No Hit
TTTTTGTTTTGTTAGGGTTTAAATGTATACCCAAAGACAAAAGAAAATTG6130.1178220790503502No Hit
CATTAAAGCAGGATAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAA6110.11743766769945184No Hit
CAGTAAACCCTGTTCCGACTACTGTCTCTCACATATCGTCAATCTTCTCG6070.11666884499765509No Hit
GCACGGGACCATGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATG6020.11570781662040917No Hit
GGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTG6000.11532340526951079No Hit
ACAATACGCTGACAGACTTTCCAATCAATAGGCCTGTTAACAGGAAGTTT5910.11359355419046813No Hit
ATGTAAAGAGTAACCCCACCTTTTTGTTTTGTTAGGGTTTAAATGTATAC5900.11340134851501894No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG5840.11224811446232383No Hit
GTCATTGGATGTTATGGGTCATTGCCACAAGATCACATCATACAGAAAAT5820.11186370311142546No Hit
GCCCAGGATGATGGGATGGGAATACAGGTGCAATTTCCGTCCGAAGGTTT5800.11147929176052712No Hit
CCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAA5770.11090267473417956No Hit
ATCCTGGGCTTTCGGAAAATTCCTATGGGAGTGGGCCTCAGCCCGTTTCT5730.11013385203238282No Hit
CATTTAAACCCTAACAAAACTAAAAGATGGGGTTACTCTTTAAATTTCAT5730.11013385203238282No Hit
GTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAACCCTAACAAAAC5720.10994164635693363No Hit
CATTGGATGTTATGGGTCATTGCCACAAGATCACATCATACAGAAAATCA5710.10974944068148444No Hit
GAGTAACCCCACCTTTTTGTTTTGTTAGGGTTTAAATGTATACCCAAAGA5640.10840400095334016No Hit
CTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTG5620.10801958960244178No Hit
GTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACCGC5600.10763517825154341No Hit
AGATCACATCATACAGAAAATCAAAGAATGTTTTAGAAAACTTCCTGTTA5560.10686635554974666No Hit
GGATGATGGGATGGGAATACAGGTGCAATTTCCGTCCGAAGGTTTGGTAC5540.1064819441988483No Hit
GGATGTTATGGGTCATTGCCACAAGATCACATCATACAGAAAATCAAAGA5540.1064819441988483No Hit
AAACTAAAAGATGGGGTTACTCTTTAAATTTCATGGGCTATGTCATTGGA5530.10628973852339911No Hit
GGTTATCGCTGGATGTGTCTGCGGCGTTTTATCATCTTCCTCTTCATCCT5510.10590532717250074No Hit
GCGCTGAACATGGAGAACATCACATCAGGATTCCTAGGACCCCTGCTCGT5500.10571312149705156No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGAAGAGGA5430.10436768176890727No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCCGTG5410.10398327041800891No Hit
AATGTAAAGAGTAACCCCACCTTTTTGTTTTGTTAGGGTTTAAATGTATA5400.10379106474255972No Hit
GAGCAGTAGTCGTGCAGGTTCTGCATGGTCCCGTGCTGGTGGTTGAAGAT5350.10283003636531379No Hit
GTCCCGTGCTGGTGGTTGAAGATCCTGGAATTAGAGGACAAACGGGCAAC5340.10263783068986461No Hit
CTCCCATAGGAATTTTCCGAAAGCCCAGGATGATGGGATGGGAATACAGG5210.10013915690902521No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)