FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488250

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488250
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences455426
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATAGAGTGTGTAAATAATGTCTGGCTTGGAAGTAATGATTAACTAAA40220.8831292021096732No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT16710.3669092234523281No Hit
CTGTAATGTCAACGACCGACCTTGAGGCATATTTCAAAGACTGTTTGTTT16470.3616394320921511No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN14880.32672706433097803No Hit
AGATAAGGGTATCAAACCTTATTATCCAGAACATTTAGTTAATCATTACT13750.30191513001014436No Hit
CTACTGTACCTGTCTTTAATCCACATTGGAAAACACCCTCTTTTCCTGAT13590.2984019357700263No Hit
CCCTTATCTAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG12620.27710319568931063No Hit
CACTAATATGGGCCTAAAGTTCAGACAATTATTGTGGTTTCACATTTCTT12490.27424872536921474No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAAACT12280.26963765792905986No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGCTT12080.26524616512891225No Hit
GGATAATAAGGTTTGATACCCTTATCTAATGGCAAATATTTGGTAACATT11670.25624360488860976No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC11660.25602403024860243No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGC11510.2527304106484917No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA11280.24768019392832205No Hit
GATATACATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGG11160.2450452982482335No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTTTAAATTTCAT10910.23955593224804908No Hit
GTGTAAATAATGTCTGGCTTGGAAGTAATGATTAACTAAATGTTCTGGAT10830.23779933512799006No Hit
CTCTATGACCGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA10270.22550315528757692No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC10130.22242911032747362No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACATAGCGCT10100.22177038640745148No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT9850.21628102040726704No Hit
TTCTTCTACTGTACCTGTCTTTAATCCACATTGGAAAACACCCTCTTTTC9700.21298740080715636No Hit
ATGTAAGACCTTGGGCAAGAAGCGGTGGGCGTTCACGGTGGTCTCCATGC9520.2090350572870236No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACCACCGTG8780.19278653392647763No Hit
ATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGCTTGGA8550.18773631720630796No Hit
CTCCAAAAGACACCAAATACTCTATGACCGTTTCTCTTCCAAAAGTGAGA8440.18532099616622677No Hit
GGGTATACATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTTTAA7920.17390311488584315No Hit
ATCCAAATGTTACCAAATATTTGCCATTAGATAAGGGTATCAAACCTTAT7830.17192694312577675No Hit
ACATTACAGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAAG7780.17082906992573987No Hit
CACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACTCACAGT7730.16973119672570297No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAAGCGGTGGGCGTTC7720.1695116220856956No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA7500.1646809800055333No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCTGTAATGTCAACGACCGACCT7390.16226565896545211No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCTTTGTATGCCTGTATT7170.15743501688528982No Hit
GCCTTAGAGTCTCCTGAGCATTGTTCACCTCACCATACTGCTCTCAGGCA7070.15523927048521605No Hit
TAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGCTTGGAAGT6980.15326309872514965No Hit
CTTATAAAGAATTTGGAGCTACTGTGGAGTTACTCTCGTTTTTGCCTTCT6930.15216522552511275No Hit
ATATTAGTGTTGACATAACTGACTACTAGGTCCCTAGATGCTGGATCTTC6900.1515065016050906No Hit
TCCTAGTACAAAGACCTTTAATCTAATCTCCTCCCCCAACTCCTCCCAGT6900.1515065016050906No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCAGTACG6650.14601713560490617No Hit
GCCTAGCAGCCATGGAAATGATGTATATTTGCGGGAGAGAACAACAGAGT6640.1457975609648988No Hit
CAATAGGCCTGTTAACAGGTAGTTTTCGAAAACAGTCTTTGATTTTCTGT6630.1455779863248914No Hit
GTTAACAGGTAGTTTTCGAAAACAGTCTTTGATTTTCTGTATGATGTGAT6420.1409669188847365No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAAT6370.1398690456846996No Hit
GTGTAAATGTATATCAGGAAAAGAGGGTGTTTTCCAATGTGGATTAAAGA6240.1370145753646037No Hit
ATCTAGAAGATCTCGTACTGAAGGAAAGAAGTCAGAAGGCAAAAACGAGA6240.1370145753646037No Hit
GGCATACAAAGGCATTAAAGCAGGATAACCACATTGTGTAAAAGGGGCAG6210.13635585144458157No Hit
CTACAAACCTTGCCAGCAAATCCACCTCCTGCCTCTACCAATCGCCAGTC6200.13613627680457419No Hit
TAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACG6120.13437967968451517No Hit
GCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTCTTTGG6100.13394053040450038No Hit
GCTTTATTCTTCTACTGTACCTGTCTTTAATCCACATTGGAAAACACCCT6060.13306223184447089No Hit
CTCACAGTCAATGAGAAAAGAAGACTGCAATTAATCATGCCTGCTAGGTT6040.13262308256445612No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACCTTCCACCAAACTCTGCAAG6030.13240350792444877No Hit
TTATTATCCAGAACATTTAGTTAATCATTACTTCCAAGCCAGACATTATT5990.13152520936441925No Hit
CCAAAAGACACCAAATACTCTATGACCGTTTCTCTTCCAAAAGTGAGACA5740.1260358433642348No Hit
GAATACAGGCATACAAAGGCATTAAAGCAGGATAACCACATTGTGTAAAA5690.12493797016419793No Hit
ATATAAGAGAGAAACAACACATAGCGCTTCATTTTGTGGGTCACCATATT5660.12427924624417577No Hit
CATTTGGATAAAACCTAGCAGGCATGATTAATTGCAGTCTTCTTTTCTCA5660.12427924624417577No Hit
TCACCATACTGCTCTCAGGCAAGCAATTCTTTGCTGGGGGGAACTAATGA5640.12384009696416103No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC5570.12230307448410939No Hit
GTCTTTGAAATATGCCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAA5490.12054647736405037No Hit
ATTTATGCCTACAGCCTCCTAGTACAAAGACCTTTAATCTAATCTCCTCC5470.12010732808403561No Hit
GAAAAGAAGACTGCAATTAATCATGCCTGCTAGGTTTTATCCAAATGTTA5450.11966817880402086No Hit
GTTTGATACCCTTATCTAATGGCAAATATTTGGTAACATTTGGATAAAAC5440.11944860416401347No Hit
CCTTTAATCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAACAG5440.11944860416401347No Hit
GTTATGTCAACACTAATATGGGCCTAAAGTTCAGACAATTATTGTGGTTT5430.1192290295240061No Hit
CTCTAAGGCATCCCGATACAAAGCTGAGGCGGTATCTAGAAGATCTCGTA5420.11900945488399872No Hit
GACCCATAACATCCAATGACATAGCCCATGAAATTTAAAGAGTAACCCCA5380.11813115632396921No Hit
GTTAGTATTCCTTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTATTC5300.11637455920391018No Hit
GTCATAGAGTATTTGGTGTCTTTTGGAGTGTGGATTCGCACTCCTCCAGC5230.11483753672385853No Hit
GTTCTGGATAATAAGGTTTGATACCCTTATCTAATGGCAAATATTTGGTA5200.1141788128038364No Hit
CTAAGAAACTGAGCCATGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT5140.11286136496379215No Hit
GTCATAGGCCATCAGCGCATGCGTGGAACCTTTTTGGCTCCTCTGCCGAT5120.11242221568377739No Hit
TCCTTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACTG5090.11176349176375525No Hit
GTCAATGAGAAAAGAAGACTGCAATTAATCATGCCTGCTAGGTTTTATCC5070.1113243424837405No Hit
GGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTCTGTGTAAACAATACC5070.1113243424837405No Hit
TCTAGGGACCTAGTAGTCAGTTATGTCAACACTAATATGGGCCTAAAGTT4990.10956774536368147No Hit
CCAAATACTCTATGACCGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGA4870.10693284968359294No Hit
ATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGCTTGGAAG4870.10693284968359294No Hit
GCCTATGACCAAGCCCCAGCCAGTGGGGGTTGCGTCAGCAAACACTTGGC4870.10693284968359294No Hit
GTTTTATCCAAATGTTACCAAATATTTGCCATTAGATAAGGGTATCAAAC4860.10671327504358558No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCACATAACTGAAAGCCAAA4860.10671327504358558No Hit
CCTTTACCCCGTTGCCCGGCAACGGCCAGGTCTGTGCCAAGTGTTTGCTG4860.10671327504358558No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA4850.10649370040357818No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC4840.10627412576357081No Hit
GTGTGTAAATAATGTCTGGCTTGGAAGTAATGATTAACTAAATGTTCTGG4790.10517625256353393No Hit
CTTCCATAGAGTGTGTAAATAATGTCTGGCTTGGAAGTAATGATTAACTA4780.10495667792352656No Hit
CTTCTAGATACCGCCTCAGCTTTGTATCGGGATGCCTTAGAGTCTCCTGA4770.10473710328351916No Hit
GGTTTGTAGTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCTCCGT4750.10429795400350442No Hit
GCAATTAATCATGCCTGCTAGGTTTTATCCAAATGTTACCAAATATTTGC4750.10429795400350442No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCTTCAACCACC4700.10320008080346751No Hit
GTGTAAACAATACCTGAACCTTTACCCCGTTGCCCGGCAACGGCCAGGTC4690.10298050616346015No Hit
TCCTAATATACATTTACACCAAGACATTATCAAAAAATGTGAACAATTTG4630.10166305832341588No Hit
GTACAAAGACCTTTAATCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTA4620.1014434836834085No Hit
GTCTTTAAACAAACAGTCTTTGAAATATGCCTCAAGGTCGGTCGTTGACA4590.10078475976338637No Hit
GGCTATGTCATTGGATGTTATGGGTCCTTGCCTCAAGATCACATCATACA4580.10056518512337898No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)