FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002488256

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488256
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences252791
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTACGTGTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATA16900.6685364589720362No Hit
CTCTCACAGTTAACGAAAAAAGGAGATTAAAATTGATTATGCCTGCTAGG10820.42802156722351664No Hit
CTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGATTATGCCTGCTAGG9630.3809471065030005No Hit
CTGTAAGAGGGCCTACATATTGTTGACATCTATTGATAATATCCTCTTTC9460.3742221835429268No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGATC8110.3208183835658706No Hit
ATCCTAACCGTACCAAATATTTGCCCTTAGATAAAGGCATTAAACCTTAT8080.3196316324552694No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC7990.3160713791234656No Hit
GTGTAAAGGGAGCGGCAAAGCCCAGAAGCCCCACAATTCTTTGACATACT7840.3101376235704594No Hit
CCCTTTACACAATGTGGTTACCCAGCATTAATGCCTTTGTATGCATGTAT7680.3038082843139194No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA7300.28877610357963696No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG6570.25989849322167324No Hit
CATTTAAACCCTACTAAAACTAAACGTTGGGGCTACTCCCTTCACTTCAT6410.25356915396513324No Hit
GGTTAGGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTTTTTTCG6380.252382402854532No Hit
GGATATTATCAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAACG6030.23853697323085077No Hit
GCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT5580.22073570657183206No Hit
ACACATAGTTAATCATTACTTCCAAACTAGGCATTATTTACATACTCTGT5530.21875778805416332No Hit
AGTCTGTACAACATCTTGAATCCCTTTTTACCGCTGTTACCAATTTTCTT5300.20965936287288708No Hit
GTCTTTAAATACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA5180.2049123584304821No Hit
ATATTCTCCTGCTGGTGGCTCCAGTTCCGGAACAGTAAACCCTGTTCCGA4770.18869342658559837No Hit
GGAAAATATAGGCCCCTCACTCTGGGATCTAGCAGAGCTTGGTGGAATGT4760.18829784288206464No Hit
CTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCTTG4720.18671550806792964No Hit
TCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAATACACAGTCTTTGA4620.18275967103259214No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN4610.1823640873290584No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTATAAGGCC4550.1799905851078559No Hit
GGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTTTTTTCGTTAAC4460.17643033177605216No Hit
GCGGTAGGAGTGCGAATCCACACTCCAAAAGACACCAAATACTCAAGAAC4380.17326566214778216No Hit
GTTCTATCCTAACCGTACCAAATATTTGCCCTTAGATAAAGGCATTAAAC4350.1720789110371809No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTTTACC4330.17128774363011343No Hit
GGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATATTTGGTACGGTT4230.16733190659477593No Hit
GTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATGTGTTCAGG4180.1653539880771072No Hit
GTATACATGCATACAAAGGCATTAATGCTGGGTAACCACATTGTGTAAAG4130.16337606955943842No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA4120.1629804858559047No Hit
GCATCAAACCTCGACAAGGCATGGGGACGAATCTTTCTGTTCCCAATCCT3980.1574423140064322No Hit
GCACTAAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCAC3970.15704673030289842No Hit
GGACTCGTGCAGGTCTTGCATGGTCCCGTGCTGGTAGTTGATGTTCCTGG3960.1566511465993647No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC3940.1558599791922972No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTAAGGCA3840.1519041421569597No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA3820.15111297474989221No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA3780.14953063993575721No Hit
GTAGTAAGCTATGTCAATGTTAATATGGGCCTAAAAATCAGACAACTATT3640.14399246808628471No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA3620.14320130067921724No Hit
GCACGATTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTGTTGCTGTACA3590.14201454956861598No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTC3580.1416189658650822No Hit
ATTTATAGGAAGTTTCCGAAAGCATTGTTTGATTTTATGTACAATATGTT3560.14082779845801474No Hit
GCATGGGAGGTTGGTCTTCCAAACCTCGACAAGGCATGGGGACGAATCTT3560.14082779845801474No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG3550.14043221475448098No Hit
GTGGTAAGGTACCCCAACTTCCAATTACATATCCCATGAAGTGAAGGGAG3490.13805871253327848No Hit
ACAATATGTAGGCCCTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGA3490.13805871253327848No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG3450.13647637771914348No Hit
ATGTAGGCCCTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGATTATG3450.13647637771914348No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC3430.13568521031207598No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTCTA3400.13449845920147474No Hit
GTATGTAAATAATGCCTAGTTTGGAAGTAATGATGAACTACGTGTTCAGG3390.13410287549794098No Hit
ACTATGTGTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATA3380.13370729179440724No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC3340.13212495698027224No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG3330.1317293732767385No Hit
ACACACAGTTAATCATTACTTCCAAACTAGGCATTATTTACATACTCTGT3310.130938205869671No Hit
CTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTCAC3300.13054262216613724No Hit
GAGTAGCCCCAACGTTTAGTTTTAGTAGGGTTTAAATGTATACCCAAAGA3240.12816911994493474No Hit
AATATGGAGAGCACCACATCAGGATTCCTAGGACCCCTGCTCGTGTTACA3210.1269823688343335No Hit
GGGTAAAGGTTCAAATATTGTTTACACAGAAAGGCCTTATAAGTTGGCGA3150.124608866613131No Hit
CCTACCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA3140.12421328290959727No Hit
CTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTCACATTCATTTGAAAG3060.12104861328132725No Hit
GGAATATGATAAAACGCCGCAGACACATCCAACGATAACCAGGACAAATT3040.12025744587425977No Hit
ATATGTTCCTGTGGTAAGGTACCCCAACTTCCAATTACATATCCCATGAA3010.11907069476365853No Hit
GGCCTACATATTGTTGACATCTATTGATAATATCCTCTTTCAAATGAATG3000.11867511106012478No Hit
GTGCACACGGTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAA2990.11827952735659102No Hit
CTCCATATTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGG2980.11788394365305727No Hit
CTCCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCG2980.11788394365305727No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGA2940.11630160883892228No Hit
ATTTACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACT2910.11511485772832103No Hit
ACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTT2890.11432369032125353No Hit
GTTTGGAAGACCAACCTCCCATGCCGTAGCTCTTGTTCCCAAGAATATGG2850.11274135550711853No Hit
GCTTTGGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG2830.11195018810005103No Hit
GTTTGATGCCGTAGCTCTTGTTCCCAAGAATATGGTGACCCACAAAATGA2800.11076343698944978No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC2800.11076343698944978No Hit
GTACAACATCTTGAATCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC2780.10997226958238228No Hit
CTGTGAGAGGGCCTACATATTGTTGACATCTATTGATAATATCCTCTTTC2760.10918110217531479No Hit
ACTGTGTGTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATA2750.10878551847178103No Hit
GGAGAATATAGGCCCCTCACTCTGGGATCTAGCAGAGCTTGGTGGAATGT2750.10878551847178103No Hit
AACTATGTGTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAAT2750.10878551847178103No Hit
GATCCATACTGCGGAACTCCTAGCAGCTTGTTTTGCTCGCAGCCGGTCTG2730.10799435106471353No Hit
CTCTAAGGCCTCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCACGAA2720.10759876736117979No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG2720.10759876736117979No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT2710.10720318365764604No Hit
CTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGATCATT2700.10680759995411229No Hit
GTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATATTTGGTA2700.10680759995411229No Hit
GCTTTATTCTTCTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCT2690.10641201625057856No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCTGATGGCC2680.10601643254704478No Hit
TGATTAACTATGTGTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGGG2670.10562084884351104No Hit
ATACAGCACTAAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTG2660.10522526513997729No Hit
GGAACATATTGTACATAAAATCAAACAATGCTTTCGGAAACTTCCTATAA2660.10522526513997729No Hit
CCTTACCACAGGAACATATTGTACATAAAATCAAACAATGCTTTCGGAAA2660.10522526513997729No Hit
CTTATATAAGACCTTGGGCAAGACCTGTTGGGCGTTCACGGTGGTTGCCA2600.10285176291877479No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT2600.10285176291877479No Hit
GACGGGGAGACCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGCCGGAACGG2590.10245617921524106No Hit
GTCCCAAACGGCCCCGAGACGGGTCGTCCGCGGGATTCAGCGCTGACGGG2580.1020605955117073No Hit
GTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTC2580.1020605955117073No Hit
CTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT2570.10166501180817356No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG2530.10008267699403856No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)