FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488258

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488258
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences542733
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTACAGGAAGTTTCCGAAAACATTGTTTGATTTTGTGTACAATATGTT32770.6037959733423248No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN23370.4305984710714108No Hit
CCCTTTACACAATGTGGTTACCCAGCATTAATGCCTTTGTATGCATGTAT22230.40959366760451277No Hit
GTCTTTAAATACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA20320.3744014091643589No Hit
GTGTAAAGGGAGCGGCAAAGCCCAGAAGCCCCACAATTCGTTGACAAACT20150.37126911391052325No Hit
CTATATTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAATTGCACT18130.3340500761884757No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGACC17880.3294437596387174No Hit
CATTTAAACCCTACTAAAACTAAACGTTGGGGCTACTCCCTTAACTTCAT17280.3183885999192973No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA16210.2986735650863316No Hit
CCTATAAATAGACCTATTGATTGGAAAGTTTGTCAACGAATTGTGGGGCT15600.2874341527049212No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA15060.2774845089574432No Hit
GTCTGTAAATACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA14590.2688246338438975No Hit
ATCCTAACCGTACCAAATATTTGCCCTTAGATAAAGGCATTAAACCTTAT14180.26127027470229375No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA13710.25261039958874804No Hit
GTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATGTGTTCAGG13690.2522418942647674No Hit
GGTTAGGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTTTTTTCG12850.23676467065757933No Hit
CCCATCATCCAGGGAATTAGTAGTAAGCTATGTCAATGTTAATATGGGCC12770.23529064936165667No Hit
GCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT12560.23142134345985965No Hit
ATTTATAGGAAGTTTCCGAAAACATTGTTTGATTTTGTGTACAATATGTT12430.22902605885398528No Hit
GTAATCTGGAAGACCCATCATCCAGGGAATTAGTAGTAAGCTATGTCAAT12410.22865755353000464No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC12290.22644652158612064No Hit
GGATATTATCAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAACG12160.22405123698024626No Hit
CTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGATTATGCCTGCTAGG11780.2170496358246136No Hit
GTATACATGCATACAAAGGCATTAATGCTGGGTAACCACATTGTGTAAAG11600.21373308790878756No Hit
AGTCTGTACAACATCTTGAATCCCTTTTTACCGCTGTTACCAATTTTCTT11350.20912677135902924No Hit
CCCGCCAGGTCTTGCCCAAGGTCTTACATAAGAGGACTCTTGGACTCTCA11300.20820550804907753No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGATC10820.1993613802735415No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTTTTTCCTTCTATTCG10810.19917712761155118No Hit
GTGGTAAGGTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAG10350.1907015051599958No Hit
TCCAAATTACTTCCCACCCAGGTGGCCAGATTCATCAACTCACCCCAACA10100.18609518861023744No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTACCACAG9860.18167312472246944No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC9830.18112036673649842No Hit
GGGTAAAGGTTCATATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA9630.17743531349669175No Hit
ATATGTTCCTGTGGTAAGGTACCCCAACTTCCAATTACATATCCCATGAA9530.1755927868767884No Hit
GTAAATACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGC9320.17172348097499138No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGA9150.16859118572115572No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG9140.16840693305916538No Hit
CCCACGTGTCCTGGCCAAAATTTGCAGTCCCCAACCTCCAATCACTCACC9030.1663801537772717No Hit
GGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTTTTTTCGTTAAC8730.16085257391756166No Hit
TCCAGAGAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATG8380.15440373074789998No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTTTACC8240.1518241934800353No Hit
GGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATATTTGGTACGGTT8230.15163994081804497No Hit
ACCCATCTCGGGGCCGTTTGGGACTCTACCGTCCCCTTCTTCGTCTGCCG8170.15053442484610297No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA8120.14961316153615128No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTCTA8080.14887615088818995No Hit
GTTCTATCCTAACCGTACCAAATATTTGCCCTTAGATAAAGGCATTAAAC7860.1448225923244026No Hit
GAAACATAGAGGTTCCTTGAGCAGGAATCGTGCAGGTCTTGCATGGTCCC7720.14224305505653792No Hit
GTAGTAAGCTATGTCAATGTTAATATGGGCCTAAAAATCAGACAACTATT7710.1420588023945476No Hit
GACCTTTAATCTAGTCTCCTCCCCCAACTCCTCCCAGTCTGTAAATACAC7630.14058478109862493No Hit
CCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAAGAGTCCTCTTATGT7580.13966351778867325No Hit
ACACTTGGGTGCTCCCCCTAGAAAATTGAGAGAAGTCCACCACGAGTCTA7570.13947926512668293No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTAAGGCA7500.13818949649275059No Hit
CTTCCAGAGAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTA7420.13671547519682792No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT7280.13413593792896322No Hit
AGTTAAGGGAGTAGCCCCAACGTTTAGTTTTAGTAGGGTTTAAATGTATA7230.13321467461901154No Hit
CCCAAGTGTCCTGGCCAAAATTTGCAGTCCCCAACCTCCAATCACTCACC7210.13284616929503087No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC7090.13063513735114687No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG7090.13063513735114687No Hit
CTGTAAGAGGGCCTACATATTGTTGACATCTATTGATAATATCCTCTTTC7050.12989812670318554No Hit
ATGTAGGCCCTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGATTATG7010.1291611160552242No Hit
ACACACAATAGCTTGCCTTAGTGCTGTATGGTGAGGTGAACAATGTTCCG6940.12787134742129186No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC6890.1269500841113402No Hit
ATTTTGTGGTCTGTAAGCGGGAGGAGTGCGAATCCACACTCCAAAAGACA6880.12676583144934986No Hit
GTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATATTTGGTA6830.1258445681393982No Hit
GTTTAAATGTATACCCAAAGATAAAAGAAAATTGGTAACAGCGGTAAAAA6750.12437054684347552No Hit
GAGACGGGTCGTCCGCGGGATTCAGCGCCGACGGGACGTAGACAAAGGAC6590.12142250425163016No Hit
GTGCACACGGTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAA6570.1210539989276495No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCTGATGGCC6510.1199484829557075No Hit
CCTGTTGGGCGTTCACGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGA6410.11810595633580416No Hit
ACAATATGTAGGCCCTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGA6380.11755319834983316No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG6290.11589492439192015No Hit
CTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTCAC6220.1146051557579878No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT6190.11405239777201681No Hit
ATGTAAGACCTTGGGCAAGACCTGGCGGGCGTTCACGGTGGTCTCCATGC6190.11405239777201681No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAG6180.11386814511002646No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC6180.11386814511002646No Hit
GTGCTGTATGGTGAGGTGAACAATGTTCCGGAGACTCTAAGGCCTCCCGA6110.11257837647609414No Hit
GTACAACATCTTGAATCCCTTTTTACCGCTGTTACCAATTTTCTTTTATC6070.11184136582813281No Hit
CACACAATAGCTTGCCTTAGTGCTGTATGGTGAGGTGAACAATGTTCCGG6010.11073584985619081No Hit
CTACGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAATTGCACT5980.1101830918702198No Hit
CCTTTACCCCGTTGCTCGGCAACGGTCAGGTCTTTGCCAAGTGTTTGCTG5930.10926182856026813No Hit
CTCAAGAACAGTTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA5930.10926182856026813No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC5860.10797205992633578No Hit
CTTATATAAAATACCAGCCTTCCAGAGAGTATGTAAATAATGCCTAGTTT5740.10576102798245178No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT5710.10520826999648077No Hit
GCCTAATCATCTCATGTTCATGTCCCACTGTTCAAGCCTCCAAGCTGTGC5700.10502401733449045No Hit
GGTTCATATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTG5680.10465551201050978No Hit
CCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGACA5670.10447125934851943No Hit
ATACAGCACTAAGGCAAGCTATTGTGTGTTGGGGTGAGTTGATGAATCTG5660.10428700668652911No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG5650.10410275402453877No Hit
CCACCAAATGCCCCTATCTTATCAACACTTCCGGAAACTACTGTTGTTAG5650.10410275402453877No Hit
GCTCTGTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCA5610.10336574337657743No Hit
GACGGGGAGACCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGCCGGAACGG5470.10078620610871275No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)