FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488259

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488259
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences542733
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTACAGGAAGTTTCCGAAAACATTGTTTGATTTTGTGTACAATATGTT31360.5778163480016877No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN23230.4280189338035461No Hit
CCCTTTACACAATGTGGTTACCCAGCATTAATGCCTTTGTATGCATGTAT20740.3821400209679529No Hit
GTGTAAAGGGAGCGGCAAAGCCCAGAAGCCCCACAATTCGTTGACAAACT20290.3738486511783879No Hit
GTCTTTAAATACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA18590.34252569864003113No Hit
CTATATTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAATTGCACT18020.33202329690658205No Hit
CATTTAAACCCTACTAAAACTAAACGTTGGGGCTACTCCCTTAACTTCAT16060.29590977515647654No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA16040.2955412698324959No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGACC15920.2933302378886119No Hit
GTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATGTGTTCAGG14660.2701144024778298No Hit
CCTATAAATAGACCTATTGATTGGAAAGTTTGTCAACGAATTGTGGGGCT14560.26827187585792645No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA14390.26513958060409076No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA14350.26440256995612943No Hit
ATCCTAACCGTACCAAATATTTGCCCTTAGATAAAGGCATTAAACCTTAT14110.2599805060683614No Hit
GTCTGTAAATACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA13670.2518733889407867No Hit
ATTTATAGGAAGTTTCCGAAAACATTGTTTGATTTTGTGTACAATATGTT12990.23934420792544403No Hit
GGTTAGGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTTTTTTCG12800.23584340734762765No Hit
GTAATCTGGAAGACCCATCATCCAGGGAATTAGTAGTAAGCTATGTCAAT12230.2253410056141786No Hit
GGATATTATCAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAACG11820.21778664647257492No Hit
CCCATCATCCAGGGAATTAGTAGTAAGCTATGTCAATGTTAATATGGGCC11710.21575986719068124No Hit
CTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGATTATGCCTGCTAGG11670.2150228565427199No Hit
CCCGCCAGGTCTTGCCCAAGGTCTTACATAAGAGGACTCTTGGACTCTCA11330.20875826603504855No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC11320.20857401337305823No Hit
GTATACATGCATACAAAGGCATTAATGCTGGGTAACCACATTGTGTAAAG11290.2080212553870872No Hit
AGTCTGTACAACATCTTGAATCCCTTTTTACCGCTGTTACCAATTTTCTT11020.20304643351334817No Hit
GCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT10940.2015724122174255No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTCTA10590.19512356904776382No Hit
ACCCATCTCGGGGCCGTTTGGGACTCTACCGTCCCCTTCTTCGTCTGCCG10230.1884904732161118No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTACCACAG10150.18701645192018912No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTTTTTCCTTCTATTCG9740.17946209277858544No Hit
GGGTAAAGGTTCATATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA9720.17909358745460477No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC9610.1770668081727111No Hit
ATATGTTCCTGTGGTAAGGTACCCCAACTTCCAATTACATATCCCATGAA9550.17596129220076906No Hit
GTGGTAAGGTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAG9470.1744872709048464No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGA9300.1713549756510107No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG9140.16840693305916538No Hit
TCCAAATTACTTCCCACCCAGGTGGCCAGATTCATCAACTCACCCCAACA9020.16619590111528137No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGATC8960.16509038514333937No Hit
GGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATATTTGGTACGGTT8590.158273036649697No Hit
GTAGTAAGCTATGTCAATGTTAATATGGGCCTAAAAATCAGACAACTATT8520.15698326801576465No Hit
GTTCTATCCTAACCGTACCAAATATTTGCCCTTAGATAAAGGCATTAAAC8480.15624625736780332No Hit
GGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTTTTTTCGTTAAC8070.14869189822619963No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT8000.14740212959226728No Hit
TCCAGAGAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATG7900.14555960297236395No Hit
GTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATATTTGGTA7530.1387422544787216No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTTTACC7370.13579421188687624No Hit
CTGTAAGAGGGCCTACATATTGTTGACATCTATTGATAATATCCTCTTTC7310.13468869591493424No Hit
GTAAATACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGC7280.13413593792896322No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTAAGGCA7240.1333989272810019No Hit
GTTTAAATGTATACCCAAAGATAAAAGAAAATTGGTAACAGCGGTAAAAA7200.13266191663304056No Hit
CCCAAGTGTCCTGGCCAAAATTTGCAGTCCCCAACCTCCAATCACTCACC7150.13174065332308887No Hit
CCTTTACCCCGTTGCTCGGCAACGGTCAGGTCTTTGCCAAGTGTTTGCTG7070.1302666320271662No Hit
GTTTGGGACTCTACCGTCCCCTTCTTCGTCTGCCGTTCCGGCCGACCACG6980.1286083580692532No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA6940.12787134742129186No Hit
GTACAACATCTTGAATCCCTTTTTACCGCTGTTACCAATTTTCTTTTATC6810.12547606281541754No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC6720.12381778885750452No Hit
GCTCTGTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCA6630.1221595148995915No Hit
CCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAAGAGTCCTCTTATGT6590.12142250425163016No Hit
CTTCCAGAGAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTA6520.12013273561769784No Hit
ATGTAGGCCCTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGATTATG6520.12013273561769784No Hit
ATTTTGTGGTCTGTAAGCGGGAGGAGTGCGAATCCACACTCCAAAAGACA6500.11976423029371717No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG6480.11939572496973651No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG6440.11865871432177517No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC6440.11865871432177517No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCTGATGGCC6300.11607917705391049No Hit
GAGACGGGTCGTCCGCGGGATTCAGCGCCGACGGGACGTAGACAAAGGAC6260.11534216640594916No Hit
CTTATATAAAATACCAGCCTTCCAGAGAGTATGTAAATAATGCCTAGTTT6250.11515791374395881No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT6210.11442090309599748No Hit
GAAACATAGAGGTTCCTTGAGCAGGAATCGTGCAGGTCTTGCATGGTCCC6210.11442090309599748No Hit
AGTTAAGGGAGTAGCCCCAACGTTTAGTTTTAGTAGGGTTTAAATGTATA6150.11331538712405548No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAG6140.11313113446206513No Hit
ACACTTGGGTGCTCCCCCTAGAAAATTGAGAGAAGTCCACCACGAGTCTA6030.11110435518017148No Hit
CTCAAGAACAGTTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA5960.10981458654623913No Hit
CTGCCGGACCGTGTGCACTTCGCTTCACCTCTGCACGTCGCATGGAGACC5840.10760355460235511No Hit
GTGCTGTATGGTGAGGTGAACAATGTTCCGGAGACTCTAAGGCCTCCCGA5840.10760355460235511No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCAAG5800.10686654395439378No Hit
ACAATATGTAGGCCCTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGA5770.10631378596842277No Hit
GGTTCATATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTG5750.1059452806444421No Hit
CTACGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAATTGCACT5720.10539252265847111No Hit
CCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGACA5620.10354999603856777No Hit
ACACACAATAGCTTGCCTTAGTGCTGTATGGTGAGGTGAACAATGTTCCG5590.10299723805259677No Hit
CTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTCAC5500.10133896409468376No Hit
GATCCATACTGCGGAACTCCTAGCAGCTTGTTTTGCTCGCAGCCGGTCTG5490.10115471143269342No Hit
GTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCGGGGCCG5430.10004919546075142No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)