FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488261

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488261
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences437545
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAATGATTAACTA19300.441097487115611No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACTCCCAATTACATATCCCATGAA18700.4273846118684936No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA18470.42212800969043185No Hit
GACACTGACCCGTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTT17660.4036156281068233No Hit
CTCATTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA16570.3787039047412266No Hit
ACTACATACTCTGGATAATATGGTTTGATCCCTTTATCTAAGGGCAAATA14110.3224811162280451No Hit
CTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGCGT12770.29185569484281615No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGG12180.278371367516484No Hit
GCACCGGACCATGCAAAACCTGCACAACTCCTGCTCAAGGAACCTCTATG12010.2744860528631341No Hit
CCACCTGCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA11730.26808671108114596No Hit
GGATAATATGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACATT11570.2644299443485813No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT11500.2628301089030843No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC11470.26214446514072837No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA11070.25300254830931673No Hit
GTCCGGTGCTGGTTGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC10960.25048852118067855No Hit
TGATCAACTACATACTCTGGATAATATGGTTTGATCCCTTTATCTAAGGG10670.24386063147790513No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT10320.23586145425042No Hit
GCCTAGCAGCCATGGAAATGATGTATACTTGCGGGAGAGCACGACAGAAT10140.23174759167628473No Hit
ATCTCAATGTTAGTATTCCTTGGACACATAAGGTGGGAAACTTTACGGGG9980.22809082494372007No Hit
GGTTTGTGAGTGACTGGAGATTTGGGACTGCGAATTTTGGCCAAGACACA9910.22649098949822305No Hit
CTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGA9720.2221485790033025No Hit
CTCACCAACCTGTTGTCCTCCAATTTGTCCTGGTTATCGCTGGATGTGTC9580.21894890811230847No Hit
GGACCATATTGTACAAAAAATCAAAATGTGTTTTAGGAAACTTCCTGTAA9480.21666342890445553No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTT9060.20706441623147331No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTTTTGGGGGC8850.20226490989498225No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAA8660.1979224994000617No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG8600.19655121187534996No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGGCACTCAGGCA8570.19586556811299408No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGAAACACCCGTG8460.1933515409843559No Hit
GTTGTGAGTAGGCCCTGAGCCTGAGGGCTCCATCCCAACAGTCCCCCATG8390.19175170553885887No Hit
CCCTTACAGTAAATGAAAACAGGAGACTAAAATTAATTATGCCTGCTAGG8150.1862665554400119No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC8080.18466671999451484No Hit
CTCTAAGGCCTCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAA7790.17803883029174142No Hit
GGGTAAAGGTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGA7670.17529625524231793No Hit
TCCAAAGAGTGTGTAAATAATGTCGCGTCTGGAAGTAATGATCAACTACA7580.1732393239552503No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGA7500.171410940588968No Hit
GACCAGGCCGTTGCCGAGCAACGGGGTAAAGGTTCAGATACTGTTTACTT7020.16044064039127404No Hit
TCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAATAGAAGGAAA6950.15884080494577701No Hit
GTTTCGCCCCAGACCTGCTGCGAGCAAAACAAGCGGCTAGGAGTTCCGCA6860.15678387365870938No Hit
TCCTCAGGCCATGCAGTGGAACTCCACCACTTTCCACCAAACTCTTCAAG6830.15609822989635352No Hit
GGAACATATTGTACAAAAAATCAAAATGTGTTTTAGGAAACTTCCTGTAA6740.1540412986092859No Hit
GCTCAGAATACTGTCTCTGCCATATCGTCAATCTTATCGAAGACTGGGGA6670.15244146316378887No Hit
CTCACAAACCTGTTGTCCTCCAATTTGTCCTGGTTATCGCTGGATGTGTC6650.1519843673222183No Hit
CCCTTATCTCCACCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAA6640.151755819401433No Hit
GTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGTACAATATGTT6590.15061307979750654No Hit
GTACAAAACCTACGGACGGAAACTGCACCTGTATTCCCATCCCATCATCT6490.14832760058965364No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAG6440.1471848609857272No Hit
GGTTAGTGAGTGACTGGAGATTTGGGACTGCGAATTTTGGCCAAGACACA6420.14672776514415659No Hit
GTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAA6370.14558502554023015No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG6360.14535647761944487No Hit
CAATAGGCCTGTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGT6350.14512792969865956No Hit
CATTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATT6160.14078551920373905No Hit
CATGTATACAAGCAAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC6150.14055697128295375No Hit
GTATGGATCGGCAGAGGAGACACAAAGGTTCCACGCATGCGCTGATGGCC6070.13872858791667142No Hit
ATGCAGATGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGA6010.13735730039195967No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTC5980.13667165662960382No Hit
GTGTTGGGGTGAGTTAATGAATCTAGCCACCTGGGTGGGAAGTAATTTAG5980.13667165662960382No Hit
GGGCAAAACTCATCGGGACTGACAATTCTGTCGTGCTCTCCCGCAAGTAT5890.13461472534253618No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG5870.1341576295009656No Hit
GTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTTTTGTC5840.13347198573860974No Hit
GTCCCGTGCTGGTTGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC5750.13141505445154214No Hit
ATGCAGTTGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGA5750.13141505445154214No Hit
ATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAAC5700.13027231484761567No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT5600.12798683563976276No Hit
CCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAAGAGTCCTCTTATGC5550.12684409603583632No Hit
GTTTAGCTTGTATACATGCATATAAAGGCATTAAAGCAGGATATCCACAT5530.12638700019426571No Hit
CTTCAAAGACTGTGTGTTTAATGAGTGGGAGGAGTTGGGGGAGGAGGTTA5510.12592990435269516No Hit
TCCTTGGACACATAAGGTGGGAAACTTTACGGGGCTTTACTCTTCTACGG5440.12433006890719812No Hit
GTTGATAGATGTAAGCAATTTGTGGGGCCCCTTACAGTAAATGAAAACAG5400.12341587722405696No Hit
GGCCTGGTCTGTGCCAAGTGTTTGCTGACGCAACCCCCACTGGTTGGGGC5390.12318732930327167No Hit
CCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAACTCCACCACTTT5350.1222731376201305No Hit
GGATATCCACATTGCGTGAAAGGGGCGGCAAACCCCAAAAGACCCACAAT5350.1222731376201305No Hit
GTACAACAGGCGGAGAAGCGGGCGGTAGAGCCCCAAGCGGCCCCGGGAGG5290.12090185009541875No Hit
CTGTTGTACCGACCGACCACGGGGCGCACCTCTCTTTACGCGGACTCCCC5260.12021620633306289No Hit
CCTTTATGCCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA5240.11975911049149231No Hit
GCTCTGTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCA5220.11930201464992173No Hit
CCTCTGCTCTGTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCT5200.11884491880835114No Hit
CCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAGTGTTTGCTG5150.11770217920442469No Hit
ATATAAAAGAGAGTCCACACGTAGTGCCTCATTTTGCGGGTCACCATATT5120.11701653544206883No Hit
CTCTTTGGAAGGCGGGGATCTTATATAAAAGAGAGTCCACACGTAGTGCC5040.1151881520757865No Hit
ATATGCAGGTGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA5030.1149596041550012No Hit
CCTGTAAACAGGCCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCT5010.11450250831343062No Hit
CCTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAAATACCTATGGGAG4980.11381686455107475No Hit
GGTCCTAGGAGTCCTGATGCGATGTTCTCCATGTTCGGTACAGGGTCCCC4970.11358831663028945No Hit
GGTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTA4930.11267412494714829No Hit
CGTGTGGACTCTCTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAA4900.11198848118479242No Hit
GCACAACTCCTGCTCAAGGAACCTCTATGTTTCCCTCATGTTGCTGTACA4830.1103886457392954No Hit
GGTTGGGGCTTGGCCATAGGCCATCAGCGCATGCGTGGAACCTTTGTGTC4800.10970300197693952No Hit
CTACCAATTCCCTGGATGCTGGATCTTCTAAATTACTTCCCACCCAGGTG4800.10970300197693952No Hit
CTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTGCACTTCGCTTCAC4770.10901735821458364No Hit
CCCCAAAAGACCCACAATTCGTTGACATACTTTCCAATCAATAGGCCTGT4660.10650333108594545No Hit
GAATTAGTAGTCGGCTATGTCAACGTTAATATGGGCCTAAAACTCAGACA4630.10581768732358958No Hit
GCTATTCTGTGTTGGGGTGAGTTAATGAATCTAGCCACCTGGGTGGGAAG4610.10536059148201898No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC4590.10490349564044842No Hit
CATTAAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT4580.10467494771966313No Hit
GTTTTGCTTGTATACATGCATATAAAGGCATTAAAGCAGGATATCCACAT4580.10467494771966313No Hit
GAACAAATGGCACTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCAC4560.10421785187809254No Hit
GTATGTAGTTGATCATTACTTCCAGACGCGACATTATTTACACACTCTTT4550.10398930395730725No Hit
TATCCACATTGCGTGAAAGGGGCGGCAAACCCCAAAAGACCCACAATTCG4540.10376075603652196No Hit
TCCTAGTACAAAGACCTTTAACCTAACCTCCTCCCCCAACTCCTCCCACT4490.1026180164325955No Hit
CTCCACCTGCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCG4480.10238946851181023No Hit
GTCCAAGAGTCCTCTTATGCAAGACCTTGGGCAGGTTCCCGTGGGCGTTC4440.10147527682866907No Hit
ATATTAACGTTGACATAGCCGACTACTAATTCCCTGGATGCTGGATCTTC4380.10010398930395731No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)