FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488262

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488262
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences405794
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATATGTTCAGG16350.4029137936982804No Hit
CTACTGTACCTGTCTTTAATCCTGAATGGCAAACTCCCTCTTTTCCTAAC14280.35190268954198434No Hit
GTGTAAAGGGAGCGGCAAAACCCAAAAGACCCACAATTCTTTGACATACT13430.33095610087877103No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA12500.308038068576667No Hit
ATCCTAACCTTACCAAATATTTGCCCTTAGATAAAGGCATCAAACCTTAT12250.30187730720513367No Hit
ATTTATAGGAAGCTTCCTAAAACATTGTTTGATTTTTTGTACAATATGTT12080.297687989472491No Hit
CCCTTTACACAATGTGGTTACCCAGCATTAATGCCTTTGTATGCATGTAT11260.2774806921738616No Hit
GGATATTATCAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAACG9390.2313981971147922No Hit
CATTTAAACCCTAATAAAACCAGACGTTGGGGCTACTCCCTTAACTTCAT9100.22425171392381357No Hit
GTATACATGCATACAAAGGCATTAATGCTGGGTAACCACATTGTGTAAAG8820.21735166118769622No Hit
CCTATAAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCT8460.20848016481268822No Hit
CTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGATCATGCCTGCTAGG8120.20010152934740286No Hit
ATATGTTCCTGTGGCAAGGTACCCCAACTTCCAATTACATATCCCATGAA7750.19098360251753352No Hit
GTTCAGGATAATAAGGTTTGATGCCTTTATCTAAGGGCAAATATTTGGTA7220.17792278840988285No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTGCCACAG7180.1769370665904375No Hit
TCCTAAAACATTGTTTGATTTTTTGTACAATATGTTCCTGTGGCAAGGTA7020.17299417931265618No Hit
TATCAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAACGAAAAAA6820.1680655702154295No Hit
GGTTAGGATAGAACCTAGCAGGCATGATCAATTTTAATCTCCTTTTTTCG6790.1673262788508455No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC6560.16165837838903482No Hit
GTTTAGGATAGAACCTAGCAGGCATGATCAATTTTAATCTCCTTTTTTCG6540.16116551747931215No Hit
CTTATATAAAATACCAGCCTTCCACAAAGTATGTAAATAATGCCTAGTTT6540.16116551747931215No Hit
CTTGAATCCCTTTTTACCGCTATTACCAATTTTCTTTTGTCTTTGGGTAT6530.16091908702445085No Hit
GGATAATAAGGTTTGATGCCTTTATCTAAGGGCAAATATTTGGTAAGGTT6450.15894764338556017No Hit
GCCTAGCAGCCATGGAAAAGAGGTGTATTTCCGAGAGAGGACAACAGAGT6040.1488439947362455No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTGATAATATCCTCTTTC6030.14859756428138415No Hit
CTAGTGGACCTGCCTCGTCGTCTAACAACAGTAGTTTCCGGAAGTGTTGA6030.14859756428138415No Hit
ATCTCAATGTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGG5980.14736541200707748No Hit
ACTATATGTTCAGGATAATAAGGTTTGATGCCTTTATCTAAGGGCAAATA5950.14662612064249347No Hit
CTATATGTTCAGGATAATAAGGTTTGATGCCTTTATCTAAGGGCAAATAT5750.1416975115452668No Hit
CTCTTATATAAAATACCAGCCTTCCACAAAGTATGTAAATAATGCCTAGT5540.1365224719931788No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA5540.1365224719931788No Hit
GACCTGCTGCGAGCAAAACAAGCTGCTAGGAGTTCCGCAGTATGGATCGG5510.1357831806285948No Hit
TCCACAAAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATA5220.12863669743761613No Hit
CCTACCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA5160.12715811470844812No Hit
GTTTTAGGAAGCTTCCTATAAATAGACCTATTGATTGGAAAGTATGTCAA5110.12592596243414148No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG5110.12592596243414148No Hit
CTACGGTACCTGTCTTTAATCCTGAATGGCAAACTCCCTCTTTTCCTAAC5050.12444737970497347No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGAATGGCAAACTCCCTCTTTTC5020.12370808834038945No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA4970.1224759360660828No Hit
CCTAATCTCCTCCCCCAACTCCTCCCAGTCCTTAAATACACAGTCTTTGA4920.12124378379177612No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA4880.12025806197233078No Hit
GCACTAAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCAC4830.11902590969802412No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC4750.11705446605913346No Hit
ATTATCAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAACGAAAA4710.11606874423968812No Hit
GGATAGAACCTAGCAGGCATGATCAATTTTAATCTCCTTTTTTCGTTAAC4710.11606874423968812No Hit
CCTTTACCCCGTTGCTCGGCAACGGTCAGGTCTTTGCCAAGTGTTTGCTG4690.11557588332996545No Hit
GTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTC4610.11360443969107478No Hit
AGTCTGTACAACATCTTGAATCCCTTTTTACCGCTATTACCAATTTTCTT4600.11335800923621346No Hit
GGTCTATTTATAGGAAGCTTCCTAAAACATTGTTTGATTTTTTGTACAAT4500.11089370468760011No Hit
GTCCCCTAGAAGAAGAACTCCCTCGCCTCGCAGACGAAGGTCTCAATCGC4490.11064727423273878No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG4440.1094151219584321No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG4430.10916869150357077No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTCTA4380.10793653922926412No Hit
GGAACATATTGTACAAAAAATCAAACAATGTTTTAGGAAGCTTCCTATAA4360.10744367831954144No Hit
CTTTAATCCTGAATGGCAAACTCCCTCTTTTCCTAACATTCATTTGAAAG4250.10473294331606678No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC4160.10251506922231478No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA4140.1020222083125921No Hit
ATTTACATACTTTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACT4140.1020222083125921No Hit
TAAATACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCT4060.10005076467370143No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)