FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488263

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488263
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences405794
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATATGTTCAGG19760.4869465788059951No Hit
ATTTATAGGAAGCTTCCTAAAACATTGTTTGATTTTTTGTACAATATGTT14400.35485985500032036No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA12720.31345953858361636No Hit
CTACTGTACCTGTCTTTAATCCTGAATGGCAAACTCCCTCTTTTCCTAAC12560.30951665130583494No Hit
CCCTTTACACAATGTGGTTACCCAGCATTAATGCCTTTGTATGCATGTAT11410.2811771489967816No Hit
GTGTAAAGGGAGCGGCAAAACCCAAAAGACCCACAATTCTTTGACATACT10750.2649127389759336No Hit
ATCCTAACCTTACCAAATATTTGCCCTTAGATAAAGGCATCAAACCTTAT10620.26170914306273624No Hit
CATTTAAACCCTAATAAAACCAGACGTTGGGGCTACTCCCTTAACTTCAT9030.22252670073978423No Hit
CCTATAAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCT8760.21587307845852824No Hit
GGATATTATCAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAACG8650.21316234345505353No Hit
GTATACATGCATACAAAGGCATTAATGCTGGGTAACCACATTGTGTAAAG8470.20872659526754955No Hit
GTTCAGGATAATAAGGTTTGATGCCTTTATCTAAGGGCAAATATTTGGTA8350.20576942980921353No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTGCCACAG8350.20576942980921353No Hit
CTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGATCATGCCTGCTAGG8110.19985509889254155No Hit
ATATGTTCCTGTGGCAAGGTACCCCAACTTCCAATTACATATCCCATGAA7410.18260496705224818No Hit
TCCTAAAACATTGTTTGATTTTTTGTACAATATGTTCCTGTGGCAAGGTA7330.18063352341335753No Hit
GTTTAGGATAGAACCTAGCAGGCATGATCAATTTTAATCTCCTTTTTTCG7210.1776763579550215No Hit
CTATATGTTCAGGATAATAAGGTTTGATGCCTTTATCTAAGGGCAAATAT7150.1761977752258535No Hit
CTTATATAAAATACCAGCCTTCCACAAAGTATGTAAATAATGCCTAGTTT7150.1761977752258535No Hit
GGATAATAAGGTTTGATGCCTTTATCTAAGGGCAAATATTTGGTAAGGTT6970.1717620270383495No Hit
GGTTAGGATAGAACCTAGCAGGCATGATCAATTTTAATCTCCTTTTTTCG6930.17077630521890416No Hit
CTTGAATCCCTTTTTACCGCTATTACCAATTTTCTTTTGTCTTTGGGTAT6860.16905129203487482No Hit
ACTATATGTTCAGGATAATAAGGTTTGATGCCTTTATCTAAGGGCAAATA6560.16165837838903482No Hit
CTCTTATATAAAATACCAGCCTTCCACAAAGTATGTAAATAATGCCTAGT6260.15426546474319483No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA6190.15254045155916549No Hit
GTTTTAGGAAGCTTCCTATAAATAGACCTATTGATTGGAAAGTATGTCAA5990.1476118424619388No Hit
TATCAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAACGAAAAAA5940.14637969018763214No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG5910.14564039882304813No Hit
CTAGTGGACCTGCCTCGTCGTCTAACAACAGTAGTTTCCGGAAGTGTTGA5880.14490110745846416No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC5790.14268323336471214No Hit
ATCTCAATGTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGG5760.14194394200012814No Hit
GCCTAGCAGCCATGGAAAAGAGGTGTATTTCCGAGAGAGGACAACAGAGT5560.13701533290290147No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC5420.1335653065348428No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGAATGGCAAACTCCCTCTTTTC5390.1328260151702588No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG5390.1328260151702588No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTCTA5300.13060814107650678No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTGATAATATCCTCTTTC5270.1298688497119228No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA5260.12962241925706147No Hit
ATTATCAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAACGAAAA5180.1276509756181708No Hit
ATATAAAATACCAGCCTTCCACAAAGTATGTAAATAATGCCTAGTTTGGA5080.12518667106955747No Hit
GGAACATATTGTACAAAAAATCAAACAATGTTTTAGGAAGCTTCCTATAA4980.12272236652094413No Hit
TCCACAAAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATA4970.1224759360660828No Hit
CCTACCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA4960.12222950561122146No Hit
GACCTGCTGCGAGCAAAACAAGCTGCTAGGAGTTCCGCAGTATGGATCGG4870.12001163151746945No Hit
GTTTGGAAGTAATGATTAACTATATGTTCAGGATAATAAGGTTTGATGCC4810.11853304878830144No Hit
GCACTAAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCAC4790.11804018787857878No Hit
GGTCTATTTATAGGAAGCTTCCTAAAACATTGTTTGATTTTTTGTACAAT4750.11705446605913346No Hit
GTCCCCTAGAAGAAGAACTCCCTCGCCTCGCAGACGAAGGTCTCAATCGC4680.11532945287510413No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA4680.11532945287510413No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGCGGTAAAAA4660.11483659196538144No Hit
GGATAGAACCTAGCAGGCATGATCAATTTTAATCTCCTTTTTTCGTTAAC4650.11459016151052011No Hit
CCTTTACCCCGTTGCTCGGCAACGGTCAGGTCTTTGCCAAGTGTTTGCTG4620.11385087014593612No Hit
ATAAAATACCAGCCTTCCACAAAGTATGTAAATAATGCCTAGTTTGGAAG4580.11286514832649078No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGTCCGTGTG4520.1113865655973228No Hit
ATTTACATACTTTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACT4380.10793653922926412No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAG4320.10645795650009611No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC4280.10547223468065077No Hit
AGTCTGTACAACATCTTGAATCCCTTTTTACCGCTATTACCAATTTTCTT4200.1035007910417601No Hit
GTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTC4200.1035007910417601No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG4140.1020222083125921No Hit
ATAATAAGGTTTGATGCCTTTATCTAAGGGCAAATATTTGGTAAGGTTAG4120.10152934740286944No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)