FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488264

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488264
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences549096
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN27530.5013695237262701No Hit
CTTTTTACCTCTATTACCAATTTTCTTTTGTCTTTGGGCATACATTTAAA25540.46512813788481433No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC22410.40812535512915776No Hit
CATTTAAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTTAT22410.40812535512915776No Hit
CTTTATACCTCTATTACCAATTTTCTTTTGTCTTTGGGCATACATTTAAA18520.3372816410973673No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGTCTAGTTT16810.30613954572606616No Hit
CTATCAATAATGTCCTCCTGTAAATGAATGTTAGGAAAGGAGGGAGTTTG16400.2986727275376255No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGACAAAGGCATTAAACCGTAT15460.2815536809592494No Hit
CCCTTAACTTTATGGGATATGTAATTGGAAGTTGGGGTACTTTACCGCAG14110.25696781619243264No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA13470.24531229511779362No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT13400.24403747250025498No Hit
GTATGTAAATAATGTCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG12860.23420312659352827No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA12190.2220012529685155No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGGCCCACAATTCTTTGACATACT11660.21234902457858007No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG11640.2119847895449976No Hit
ATATGTTCCTGCGGTAAAGTACCCCAACTTCCAATTACATATCCCATAAA11460.20870667424275538No Hit
TTATTAATAGATGTCAACAATACGTGGGCCCTCTTACAATTAATGAAAAA11190.20378950128939205No Hit
ATTTACGAAAACATTGCTTGAGTTTTAGTACAATATGTTCCTGCGGTAAA10740.19559421303378643No Hit
CTTCAAAGACTGTGTGTTTAAAGACTGGGAGGAGTTGGGGGAGGAGATTA10440.19013068753004939No Hit
GTACAATATGTTCCTGCGGTAAAGTACCCCAACTTCCAATTACATATCCC10150.18484927954310357No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTTAAATGTATG10070.1833923394087737No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC9720.17701822632108047No Hit
TTATTGATAGATGTCAACAATACGTGGGCCCTCTTACAATTAATGAAAAA9300.1693692906158486No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA9060.16499847021285896No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC8890.16190247242740796No Hit
GGTTAGGATAAAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA8810.16044553229307806No Hit
CTCCAAAAGATACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA8780.15989917974270437No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTTAGGAAAGGAGGGAGTTTG8740.15917070967553945No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGTCTAGT8720.15880647464195696No Hit
GGGTAAAGGTGCAGATATTGTTGACACAGAAAGGCCTTGTAAGTTGGCGA8600.15662106444046214No Hit
CCTTTTTACCTCTATTACCAATTTTCTTTTGTCTTTGGGCATACATTTAA8500.1547998892725498No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC8370.15243236155426373No Hit
CTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATCATT8060.14678671853373546No Hit
GGATAATACGGTTTAATGCCTTTGTCCAAGGGCAAATATTTGGTAAGGTT7720.14059472296283346No Hit
TCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAATAGAAGGAAA7710.14041260544604223No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG7630.13895566531171236No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG7610.13859143027812987No Hit
GTCTATTTACAGGCAATTTACGAAAACATTGCTTGAGTTTTAGTACAATA7580.13804507772775615No Hit
CTCTAAGGCCTCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAA7450.1356775500094701No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC7360.134038492358349No Hit
TTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAATTTACGAAAAC7330.1334921398079753No Hit
GTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTTAAATGTATGC7260.13221731719043664No Hit
ATTTATGCCTACAGCCTCCTAGTACAAAGATCATTAACCTAATCTCCTCC7150.13021402450573305No Hit
GGTAAAAAGGGACTCAAGATGTTGTACAGACTTGGCCCCCAATACCACAT7080.1289392018881944No Hit
GGTCTATTTACAGGCAATTTACGAAAACATTGCTTGAGTTTTAGTACAAT7060.12857496685461195No Hit
GTTCAGGATAATACGGTTTAATGCCTTTGTCCAAGGGCAAATATTTGGTA7010.12766437927065577No Hit
GTTTTATCCTAACCTTACCAAATATTTGCCCTTGGACAAAGGCATTAAAC6990.1273001442370733No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC6970.12693590920349082No Hit
CTATTACCAATTTTCTTTTGTCTTTGGGCATACATTTAAACCCTAATAAA6920.12602532161953467No Hit
GTATTATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGACATTATT6900.12566108658595218No Hit
CATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA6790.1236577939012486No Hit
CCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT6740.12274720631729243No Hit
ATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGACATTATTTACAT6630.12074391363258884No Hit
ATTTACAGGCAATTTACGAAAACATTGCTTGAGTTTTAGTACAATATGTT6590.1200154435654239No Hit
CTCTTACAATTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG6450.1174657983303466No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT6430.11710156329676413No Hit
CAATTACATATCCCATAAAGTTAAGGGAGTAGCCCCAACGTTTGGTTTTA6410.11673732826318166No Hit
ATGTAATTGGAAGTTGGGGTACTTTACCGCAGGAACATATTGTACTAAAA6390.11637309322959918No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTC6320.11509827061206056No Hit
CAATAGGTCTATTTACAGGCAATTTACGAAAACATTGCTTGAGTTTTAGT6320.11509827061206056No Hit
GCTTTGGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCATCTGTGG6280.11436980054489561No Hit
TCCACAGAGTATGTAAATAATGTCTAGTTTTGAAGTAATGATTAACTGCA6230.11345921296093944No Hit
ACTTTATGGGATATGTAATTGGAAGTTGGGGTACTTTACCGCAGGAACAT6210.11309497792735697No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC6180.11254862537698326No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAAC6150.11200227282660954No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG6130.11163803779302708No Hit
GCTTAGATTGTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACAT5850.1065387473228725No Hit
CTTTTACACAATGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATA5830.10617451228929004No Hit
CTTGATTTTTAGTACAATATGTTCCTGCGGTAAAGTACCCCAACTTCCAA5770.10508180718854263No Hit
GTTTAAATGTATGCCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAA5680.10344274953742151No Hit
GGATAAAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAT5670.10326063202063028No Hit
CTCACAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC5610.10216792691988286No Hit
ATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAATAGAAGGAAAGAAGT5610.10216792691988286No Hit
TATTAATAGATGTCAACAATACGTGGGCCCTCTTACAATTAATGAAAAAA5540.10089310430234423No Hit
ACCTTGAGGCATACTTCAAAGACTGTGTGTTTAAAGACTGGGAGGAGTTG5510.10034675175197051No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)